19 results about "Hypoxic stress" patented technology

The invention discloses an application of a CHRNA3 agonist in treatment of sleep disorders. The application finds that the CHRNA3 agonist varenicline can effectively relieve the sleep disorder reaction for the first time, on this basis, the invention provides a new application of the CHRNA3 agonist varenicline in treating the sleep disorder, and meanwhile, provides a method for screening candidate drugs for treating diseases caused by hypoxia stress, and provides a new application of the CHRNA3 agonist varenicline in treating the sleep disorder. A new treatment means is provided for sleep disorder, especially sleep disorder caused by hypoxia stress.

The invention discloses a functional feed additive for relieving hypoxia stress of dairy cows in a perinatal period, and belongs to the technical field of functional feed additives. The functional feed additive is composed of amino acid chelated iron, a nutrition carrier and water, the iron content is larger than or equal to 9%, and the arginine content is larger than or equal to 10%. The recommended daily supplement dosage is 6.6 g / head, the feed is fed in three times after being mixed with basic ration, and the service cycle is from 21 days before delivery to 21 days after delivery. The additive can significantly improve the hemoglobin concentration and blood oxygen transport capacity of the perinatal dairy cow, relieve hypoxia stress, improve the lactation performance, optimize the metabolic state and enhance the anti-oxidation and anti-inflammatory capacity, is clear in action mechanism, safe and efficient, and is suitable for healthy breeding and production performance improvement of the perinatal dairy cow.

The invention provides a plateau low-pressure and low-oxygen stress diagnostic marker and application thereof, and discloses a microRNA marker miR-874-3p which can be used for diagnosing low-pressure and low-oxygen stress and related diseases caused by sudden entry into plateau. The invention also provides application of the reagent for detecting the expression level of the miR-874-3p in preparation of a product for diagnosing diseases related to low-pressure and low-oxygen stress, wherein the low-pressure and low-oxygen stress is caused by sudden entry into the plateau.

Cancers develop specific metabolic adaptations such as the opportunistic utilization of acetate as an alternative carbon source and the generation of acetyl-CoA mediated by ACSS-2, which is upregulated in response to nutrient and hypoxic stress. This pathway can fuel various processes involving acetyl-CoA, including de novo fatty acid synthesis and protein acetylation. Provided herein are methods of treating a cancerous disease in a subject using compounds that inhibit ACSS-2.

The invention provides an application of an exosome blocker in treatment of memory down-regulation. The invention finds that the expression of miR-24 is up-regulated after hypoxia stress for the first time, the miR-24 can be used for effectively diagnosing whether a subject is subjected to hypoxia stress or not, and the invention also finds that the miR-24 blocking agent can be used for effectively treating cognitive impairment induced by hypoxia.

According to the application, an acute hypoxia animal model is established through a plateau low-pressure oxygen cabin, and the effect of cycloastragenol on prevention of acute hypoxia movement stress mice after pretreatment is evaluated. It is found that cycloastragenol after pretreatment has no adverse effect on the body, the exercise performance of mice can be improved, the exercise time can be prolonged, and gastrocnemius muscle and myocardial tissue damage can be improved. Accumulation of LAC in serum after exhaustive exercise can be remarkably reduced, accumulation of a metabolite BUN is reduced, the activity of antioxidant enzyme T-SOD in the serum is remarkably improved, and accumulation of a lipid peroxidation product MDA in the serum is reduced. The cycloastragenol can reduce the content of inflammatory factors IL-6 and TNF-alpha in serum, improve the level of Ca < 2 + >-Mg < 2 + >-ATP enzyme and Na < + >-K < + >-ATP enzyme in gastrocnemius muscle, regulate energy metabolism and improve energy supply efficiency.

The invention discloses an application of a fructus psoraleae extract in carassius auratus gibelio hypoxia stress, and relates to the technical field of application of fructus psoraleae, and the application comprises the step of adding the fructus psoraleae extract into a feed so as to improve the hypoxia tolerance or oxidation resistance of carassius auratus gibelio. According to the invention, the fructus psoraleae extract is added into the feed, so that the purpose of improving the hypoxia tolerance or oxidation resistance of the carassius auratus gibelio is achieved.

This invention discloses a method for improving the environmental stress resistance of fish by subjecting them to intermittent hypoxia training. The method includes: maintaining dissolved oxygen (DO) in the water at normoxic levels; setting a fixed time window daily to begin deoxygenation treatment, causing DO to drop to a target hypoxic level within a specified time and maintaining this target hypoxic level for a period of time; subsequently, reoxygenating to restore DO to normoxic levels. This intermittent hypoxia training is conducted continuously, with water quality indicators maintained within a suitable range for the target fish during the training period. After the training is completed, the resistance of the target fish is evaluated. This invention is simple to operate. After intermittent hypoxia training, the LOE threshold of the target fish decreases under hypoxic stress, and their hypoxia tolerance is improved. The fish also exhibit enhanced antioxidant capacity and reduced lipid peroxidation levels under hypoxic, high-temperature, and high-ammonia nitrogen stress, providing cross-protection for fish populations growing under various environmental stresses.

The invention provides an application of circulating free mitochondrial DNA (deoxyribonucleic acid) in hypoxia stress. Through in-vitro cell experiment screening and animal in-vivo experiment verification, a research result shows that the copy number of the cf-mtDNA is increased under a low-oxygen stress condition, and part of the cf-mtDNA is released by exosome. The change of the copy number of the cf-mtDNA can indicate the hypoxia stress, and the cf-mtDNA can be used as a biomarker of the hypoxia stress.

The invention discloses an application of miRNA in diagnosis of cognitive function decline induced by hypoxia stress, the miRNA is miR-3473h-p3, the miRNA has good diagnostic efficiency, accuracy, sensitivity and specificity for diagnosis of cognitive function decline induced by hypoxia stress, can be used in effective diagnosis of cognitive function decline induced by hypoxia stress, and has good application prospects in diagnosis of cognitive function decline induced by hypoxia stress. The invention provides a brand new thought and strategy for research and development of diagnosis products related to hypoxia stress induced cognitive function decline, and has wide application prospects and important conversion significance in the technical field of diagnosis of hypoxia stress induced cognitive function decline.

The invention relates to the technical field of biology, in particular to shRNA for inhibiting TIM-3 gene expression, an immune cell and application of the shRNA. According to the shRNA molecule and the application thereof, the shRNA molecule targets TIM-3 expression, and an efficient, specific and stable silencing effect can be kept; the TIM-3 gene is silenced, so that the killing capability of immune cells on tumor cells is effectively improved; the multiplication capacity and the viability of immune cells in a low-oxygen stress or low-oxygen microenvironment are effectively improved; the multiplication capacity and the viability of immune cells in a complex immunosuppression tumor microenvironment are effectively improved.

The invention discloses a medicinal plant extract for improving the anti-stress capability of micropterus salmoides as well as a preparation method and application of the medicinal plant extract. Belongs to the technical field of aquaculture. The rosemary extract is prepared from rosemary, astragalus membranaceus and liquorice according to a specific proportion through an innovative process of composite enzymolysis, ultrasonic-assisted extraction and macroporous resin purification, and can effectively relieve temperature, water quality and transportation stress; the resistance of the micropterus salmoides to novel stress sources such as low-oxygen stress and salinity sudden change can be obviously improved. Experiments prove that the extract can significantly reduce serum cortisol and lactic acid levels and improve superoxide dismutase activity and immunoglobulin concentration under the addition amount of 0.1%, the survival rate under hypoxia stress is improved by 35% or above, the regulation recovery time under salinity sudden stress is shortened by 50%, and the content of the superoxide dismutase is reduced by 50% or above. The obvious synergistic interaction effect and the unexpected technical effect are shown.

The invention discloses an application of an exosome blocker in treatment of sleep disorders. It is found for the first time that after miR-24 secretory expression is inhibited, the hypoxia stress induced total sleep and daytime sleep duration reduction and sleep fragmentation phenomena are obviously improved. A new intervention strategy is provided for coping with sleep disorder caused by environmental stress, and the application prospect is wide.

The invention relates to the technical field of biomedicine and nutriology, and relates to a composition for targeted inhibition of lipid peroxidation to promote plateau study and application. Specifically, protein change characteristics of plateau hypoxia stress cells or mouse tissues are analyzed through a proteome technology, a lipid metabolism change rule in the plateau hypoxia stress mouse tissues is analyzed through a targeted lipid metabolome technology, and lipid peroxidation damage of hypoxia to the cells is analyzed through tests such as MDA content determination, ATP content determination and CCK8 cell activity detection. And providing a scheme of jointly supplementing iron and oleic acid to promote plateau exercise, and verifying in animal experiments. The iron and the olive oil are supplemented into the feed at the same time, so that the oxygen partial pressure and the oxyhemoglobin saturation of the mouse can be further improved while the mouse resists lipid peroxidation damage caused by high altitude hypoxia. The combined supplement of iron and olive oil can reduce tissue damage and improve the plateau taking ability of the organism, and the method is a safe and effective plateau taking promoting strategy.

The invention provides a bovine primary cardiac fibroblast hypoxia model construction method, and belongs to the technical field of cell model construction, trypsin and type II collagenase are adopted for sequentially digesting, differential attachment and the like to obtain bovine primary cardiac fibroblasts, the bovine primary cardiac fibroblasts are treated for 24-72 h under the hypoxia condition of 1% O2, 5% CO2 and 94% N2 at the inoculation cell density of 3.5 * 10 < 5 >-8 * 10 < 5 > / ml, and the bovine primary cardiac fibroblasts are obtained. And constructing the cell hypoxia model. The three-gas incubator hypoxia model based on the primary bovine myocardial fibroblasts can accurately simulate a pathological hypoxia environment, and is suitable for hypoxia stress mechanism research and key molecule screening. The model is high in physiological correlation and simple and convenient to operate, has wide applicability and reference value, and provides a reliable experimental platform for research on the bovine hypoxia myocardial diseases.

The invention discloses an application of a CHRNA3 agonist in treatment of memory down-regulation induced by low-pressure and low-oxygen stress. It is found for the first time that a CHRNA3 agonist can effectively relieve learning and memory ability decline induced by hypoxia stress, based on the regulation and control relation of miR-24 and CHRNA3, the computer-aided drug screening method, system and equipment based on miR-24 and CHRNA3 are provided, a new thought and direction are provided for treatment of learning and memory ability decline induced by hypoxia stress, and the method, the system and the equipment have good application prospects. The application prospect is wide.

The invention relates to a preparation method of an anti-hypoxia stress mixed fatty alkanol aquatic feed additive, and belongs to the technical field of aquatic feed additives. The preparation method of the aquatic feed additive comprises the following steps: 1) melting mixed fatty alkanol in hot ethanol; 2) adding a compound emulsifier of soybean lecithin and sorbitan monooleate, and performing hot melting; (3) slowly adding vegetable oil of which the temperature difference with the system temperature does not exceed 2 DEG C; 4) carrying out ultrasonic emulsification to prepare an emulsion; (5) adding the emulsion into a premix formed by corncob powder, sodium butyrate and calcium carbonate, and stirring and mixing; the natural mixed fatty alkanol and carrier mixing technology is utilized, and through the step-by-step synergistic technical means, the technical problems that existing single octacosanol is high in cost, single in effect, uneven in mixing with feed and poor in dispersity in water are solved, so that the comprehensive biological activity of the additive is remarkably improved.

The invention discloses an application of miRNA-34a-5p (micro Ribonucleic Acid-34a-5p) in diagnosis of cognitive function decline induced by hypoxia stress. According to the application, the miR-34a-5p in serum is used for diagnosing the cognitive function decline induced by the hypoxia stress for the first time, and verification shows that the miR-34a-5p has relatively high diagnosis efficiency, relatively high sensitivity and relatively high specificity, is beneficial to early screening of the cognitive function decline induced by the hypoxia stress, is beneficial for clinical doctors to judge in time and carry out corresponding intervention, and is beneficial to clinical diagnosis of the cognitive function decline induced by the hypoxia stress. Therefore, the traditional Chinese medicine composition has a wide application prospect clinically.

The invention discloses a green field fish fin ray cell line as well as a construction method and application thereof, and belongs to the technical field of freshwater fish cell culture, the green field fish fin ray cell line is named as green field fish fin ray cell line CQF, and is preserved in China Center for Type Culture Collection on April 2, 2025, and the preservation number is CCTCC NO: C202591. The cell line is derived from green field fish, and on the basis of fin rays of the green field fish, the culture method comprises the following steps: selecting the green field fish with excellent health conditions, temporarily rearing at room temperature for 2 weeks, feeding once in the morning and once in the evening, simultaneously preparing a primary culture medium and a subculture medium, treating fin ray tissues, performing primary culture on fin ray tissue cells, performing subculture, and performing cell cryopreservation and recovery. The green field fish fin ray cell line provided by the invention has the advantages of strong division ability, fast growth speed, good stability, strong repeatability, simple and easy operation, and high recovery activity, and can establish a fish hypoxia stress model as a new in vitro research tool, accelerate the breeding process of good varieties, and improve the breeding efficiency.