A SNP molecular marker related to hypoxia tolerance traits of mandarin fish and its application
A molecular marker, mandarin fish technology, applied in the field of molecular biology, can solve problems such as the reports of SNP molecular markers of genes related to hypoxia tolerance traits that have not yet been seen
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[0029] 1. The acquisition of mandarin fish populations
[0030] The fish used in this example are taken from the experimental base of the Freshwater Fisheries Research Center of the Chinese Academy of Fishery Sciences, and 100 mandarin fishes that have been cultured for about three months from the membranes have been selected to be cultured in the indoor circulating water system. At the beginning of the experiment, turn off the aeration and water intake, fill in nitrogen to discharge the oxygen in the water, measure the dissolved oxygen in the water with a dissolved oxygen meter (measured by the LDO101 probe of the American LDO HQd portable instrument), when the dissolved oxygen in the water cannot decrease (about 0.9mg / L) Finally, seal the aquarium with a transparent film to allow it to naturally reduce oxygen. During the experiment, observe the activities of the fish, and start timing when the first mandarin fish loses balance and begins to sink, and take 50 hybrid mandarin...
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