A SNP molecular marker related to hypoxia tolerance traits of mandarin fish and its application

A molecular marker, mandarin fish technology, applied in the field of molecular biology, can solve problems such as the reports of SNP molecular markers of genes related to hypoxia tolerance traits that have not yet been seen

Active Publication Date: 2022-12-02
FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

[0004] So far, there have been no reports on SNP molecular markers of genes related to hypoxia tolerance traits in mandarin fish

Method used

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  • A SNP molecular marker related to hypoxia tolerance traits of mandarin fish and its application
  • A SNP molecular marker related to hypoxia tolerance traits of mandarin fish and its application
  • A SNP molecular marker related to hypoxia tolerance traits of mandarin fish and its application

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Embodiment 1

[0029] 1. The acquisition of mandarin fish populations

[0030] The fish used in this example are taken from the experimental base of the Freshwater Fisheries Research Center of the Chinese Academy of Fishery Sciences, and 100 mandarin fishes that have been cultured for about three months from the membranes have been selected to be cultured in the indoor circulating water system. At the beginning of the experiment, turn off the aeration and water intake, fill in nitrogen to discharge the oxygen in the water, measure the dissolved oxygen in the water with a dissolved oxygen meter (measured by the LDO101 probe of the American LDO HQd portable instrument), when the dissolved oxygen in the water cannot decrease (about 0.9mg / L) Finally, seal the aquarium with a transparent film to allow it to naturally reduce oxygen. During the experiment, observe the activities of the fish, and start timing when the first mandarin fish loses balance and begins to sink, and take 50 hybrid mandarin...

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Abstract

The invention discloses a SNP molecular marker related to hypoxia resistance traits of mandarin fish and its application, including molecular marker SNP1 and molecular marker SNP2; the molecular marker SNP1 is located at the 5' from the nucleotide sequence shown in SEQ ID NO.1 The 1919th position from the end, where the base is T or C; the molecular marker SNP2 is located at the 4784th position from the 5' end of the nucleotide sequence shown in SEQ ID NO.1, where the base is T or C. The SNP loci disclosed in the present invention are significantly related to the survival rate of mandarin fish under hypoxic stress. Individuals of the SNP 1919 TT genotype and 4784 TC genotype have significantly stronger hypoxia tolerance than individuals with TC and TT genotypes. Molecular marker-assisted breeding can be carried out without restrictions on the age and sex of mandarin fish. It can be used for early breeding of mandarin fish, which can significantly promote the breeding process of mandarin fish and the survival rate under low oxygen conditions during transportation. The method is accurate and reliable. Easy to operate.

Description

technical field [0001] The invention belongs to the field of molecular biology, and in particular relates to a SNP molecular marker related to hypoxia resistance traits of mandarin fish and an application thereof. Background technique [0002] Siniperca chuatsi (Siniperca chuatsi), commonly known as sweet-scented osmanthus fish, squid and crucian carp, belongs to the family Percichthyidae and Sinipercinae of the order Perciformes. Because of its plump meat, no intermuscular spines, delicious taste, low cholesterol content and high nutritional value, it is widely cultivated in my country. According to statistics, the amount of mandarin fish cultured in my country has been increasing year by year. From 2003 to 2019, the domestic production of mandarin fish has increased from 150,000 tons to more than 300,000 tons. The main production areas of mandarin fish are Guangdong, Jiangxi, Hubei, Anhui, Jiangsu and other provinces. Among them, Guangdong has the highest production of ma...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156C12Q2600/124Y02A40/81
Inventor 丁炜东曹丽萍曹哲明邴旭文
Owner FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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