A method for analyzing whether prawns are subjected to hypoxic stress by detecting hurp1 gene expression

Abstract

The invention provides a method for analyzing hypoxia stress on shrimps by detecting HURP1 gene expression. The method comprises the steps as follows: step one, acquiring the sequence of an open reading frame of an HURP1 gene of litopenaeus vannamei, and designing and synthesizing a sequence-specific fluorescent quantitative PCR (polymerase chain reaction) primer; step two, acquiring to-be-detected blood lymphocytes of litopenaeus vannamei, extracting the total RNA (ribose nucleic acid) of the blood lymphocytes, reversely transcribing the total RNA into cDNA, and preparing a fluorescent quantitative PCR template; step three, performing fluorescent quantitative PCR analysis to analyze expression of HURP1 in the blood lymphocytes of litopenaeus vannamei. With the adoption of the method, whether litopenaeus vannamei is in a low-oxygen stress state is judged more conveniently, more quickly and more accurately by detecting expression of the HURP1 gene.

Description

technical field [0001] The invention belongs to the field of molecular biology, and in particular relates to a method for analyzing whether Litopenaeus vannamei is in a state of hypoxic stress by detecting the expression of HURP1 gene. Background technique [0002] The prawn (Litopenaeus vannamei) in the genus Penaeus is an important species of aquaculture in my country. In 2015, the global production of farmed prawns reached 3.3 million tons, of which, the output of farmed prawns in my country was 1.5 million tons, making it the largest country in the world for prawn farming. my country mainly cultures four species of prawns, Litopenaeus vannamei, Penaeus monodon, Fenneropenaeus chinensis and Marsupenaeus japonicus, among which Litopenaeus vannamei is the main cultured species, about It accounts for 90% of the total production of cultured prawns. [0003] In shrimp culture water, oxygen is consumed by various biological or abiotic factors, and the proportion of oxygen cons...

AI Technical Summary

Problems solved by technology

Insufficient dissolved oxygen can easily cause hypoxic stress to Litopenaeus vannamei, which has adverse effects on the growth rate and immune function of Litopenaeus vannamei, and often causes losses to the shrimp farming industry

Since the absorption and utilization of dissolved oxygen in water by prawns is affected by many other physical and chemical factors, such as temperature or ammonia nitrogen concentration, the amount of dissolved oxygen in water has no direct correlation with the amount of dissolved oxygen in Litopenaeus vannamei, so it is difficult to directly obtain it from aquaculture water. Determining whether the body of Litopenaeus vannamei suffers from hypoxic stress

However, there is still a lack of simple, fast and accurate methods for judging whether Litopenaeus vannamei is in a state of hypoxic stress.

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