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SNP (single nucleotide polymorphism) molecular markers related to hypoxia tolerance of rice and application of SNP molecular markers

A technology of rice and molecular breeding, applied in the field of rice breeding and molecular genetics, can solve problems such as irregular emergence, lack of seedlings, and restrictions on the development of direct-seeding rice

Pending Publication Date: 2019-02-22
INST OF CROP SCI CHINESE ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, environmental conditions such as low oxygen and low temperature during direct seeding of rice have caused irregular seedling emergence and lack of seedlings, which limit the development of direct seeding rice.

Method used

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  • SNP (single nucleotide polymorphism) molecular markers related to hypoxia tolerance of rice and application of SNP molecular markers
  • SNP (single nucleotide polymorphism) molecular markers related to hypoxia tolerance of rice and application of SNP molecular markers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1. Acquisition and Identification of SNP Molecular Markers Closely Linked to Rice Germination Resistance to Low Oxygen

[0046] 1. Acquisition of SNP molecular markers closely linked to rice germination tolerance to low oxygen

[0047] 1. From the 3,000 global rice core germplasm resources that have been deeply sequenced (3K RGP.The 3,000RiceGenome Project[J].GigaScience,2014,3:7), according to the growth period, genotype, geographical origin and rice type, etc. characteristics, and combined with other agronomic traits such as plant height, seed setting rate, etc., 582 rice materials with rich genetic variation were screened, and the screened 582 rice materials were used as test materials, including 372 indica rice, 172 japonica rice, Aro 16 , Aus 14 and Admixed 8.

[0048] 2. In June 2016, all the materials were planted in the greenhouse of the Institute of Crop Science, Chinese Academy of Agricultural Sciences, and the seedling survival rate of 582 germplasm ...

Embodiment 2

[0056] Example 2, Application of SNP Molecular Marker Closely Linked to Rice Germination Resistance to Low Oxygen

[0057] 1. Detection of seedling survival rate

[0058] In the associated population, 20 germplasm resources with significant differences in seedling survival rate under hypoxic stress conditions were randomly selected as experimental materials (Table 2). The 20 materials were all recorded in the literature "Wang Wensheng et al. Accessions of Asian cultivated rice. Nature, 2018, 557:43-49.", the public can obtain from the applicant, the biological material is only used for repeating the relevant experiments of the present invention, and cannot be used for other purposes. The survival rate of rice seedlings was detected according to the method of 2 in step 1 of Example 1. The results are shown in Table 2.

[0059] 2. Genotype detection

[0060] The genotype of the rice to be tested was detected according to the method in Step 2 of Example 1.

[0061] The result...

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Abstract

The invention discloses SNP (single nucleotide polymorphism) molecular markers related to hypoxia tolerance of rice and application of the SNP molecular markers. The SNP molecular markers provided bythe invention are located at the 30th, 096th and 391st sites of rice chromosome 3, and the polymorphism is A or G. The SNP molecular markers are significantly correlated with the survival rate of riceseedlings under hypoxic stress conditions. A rice variety with a genotype being GG at the sites is significantly stronger in resistance to hypoxic germination than that of a rice variety with the genotype being AA under hypoxic stress conditions. The invention also designs PCR primers based on the SNP sites, and the nucleotide sequences of the primers are shown in sequence 1 and sequence 2. The SNP molecular markers related to the hypoxia tolerance in rice provided by the invention are verified in natural populations that the markers can effectively predict the hypoxia germination tolerance of rice, and thus can be used for marker-assisted breeding of hypoxia tolerance and direct seeding molecular breeding in rice.

Description

technical field [0001] The invention relates to the fields of rice breeding and molecular genetics, in particular to a SNP molecular marker related to rice low-oxygen tolerance, in particular to a SNP molecular marker on rice chromosome 3 that is significantly associated with rice low-oxygen-resistant germination traits and its application. Background technique [0002] Rice is one of the three most important food crops in the world and is the main source of food for 2 / 3 of the world's population. With the rapid growth of population and economy, food production is facing enormous development pressure, and safe production of rice is crucial to China's sustainable development. In recent years, with the development of the economy, the increase of labor costs and the popularization of agricultural mechanization, direct-directed rice has become a new development direction of rice production, which has the advantages of low cost and easy operation. However, environmental conditi...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 王文生傅彬英穆罕默德·拉菲克伊·斯拉姆黎志康吴志超徐建龙王春超赵秀琴孙帆王银晓
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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