Method for detecting aphelenchoides besseyi and diagnostic reagent kit

Abstract

The invention discloses a method for detecting aphelenchoides besseyi, and the method comprises the following steps: using SEQ ID NO: 1 and SEQ ID NO: 2 in a sequence table as a primer pair to carry out polymerase chain reaction; determining whether a specific fragment is obtained or not by electrophoretic analysis; and then detecting or identifying whether the specific fragment is the aphelenchoides besseyi or not. The invention also provides a reagent kit for diagnosing the aphelenchoides besseyi disease, which contains the primer pair. The method and the reagent kit have rather high reliability and sensitivity, the operation is convenient and does not rely on experience of an operator, the accuracy is high, the practicability is strong, and the method and the reagent kit can meet the needs of fast and reliable detection and identification of the aphelenchoides besseyi before agricultural crops are planted and in the plant quarantine process.

Description

technical field [0001] The invention relates to a method for detecting or identifying the rice stem nematode and a kit for detecting or identifying the rice stem nematode. Background technique [0002] Aphelenchoides besseyi Christie, also known as Aphelenchoides besseyi Christie, is mainly host to rice and can cause rice stem-tip or white-tip disease, which occurs in all rice-producing areas across the country, generally causing a 10% reduction in yield- 20%, severe cases can reach more than 30%. It can also cause summer dwarf disease of strawberry. The leaves of the affected strawberry are shrunken and deformed, the plants are dwarfed, the flowering is reduced, and the yield is greatly reduced. At the same time, the nematode is also an important disease of corn, sweet potato, soybean, vegetables, orchids, chrysanthemums, dahlias and other crops or flowers, resulting in reduced economic value. [0003] Rice stem nematode is mainly transmitted by seeds with disease. Once i...

AI Technical Summary

Problems solved by technology

There are certain disadvantages in this method, which are mainly manifested in: 1. The rice stem-point nematode belongs to the genus S. spp., and there are many kinds of s. Rich experience; 2. There are differences among individual nematodes, which requires sufficient identification samples; 3. Morphological identification is only applicable to adults, but not to larvae

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