Dendrobe chromatogram finger print measuring method

A fingerprint and determination method technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve the effect of fast analysis speed, reliable results, and easy access to raw materials

Active Publication Date: 2010-06-09
ZHONGSHAN ZHONGZHI PHARMA GRP
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the quality control of dendrobium phenols and flavonoids using high performance liquid phase (HPLC) fingerprinting. Therefore, this method is of great significance for controlling the quality of dendrobium raw materials and products.

Method used

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  • Dendrobe chromatogram finger print measuring method
  • Dendrobe chromatogram finger print measuring method
  • Dendrobe chromatogram finger print measuring method

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Experimental program
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Embodiment Construction

[0041] Starting from the experimental goal, in the experiment we selected and compared different C 18 (ODS column) chromatographic column: Spheris-orb ODS2 (150mm*4.6mm*5μm, waters) chromatographic column, Phenomenex C 18 (250mm*4.6mm*5μm, Luna) chromatographic column, Zorbax SBC 18 (250mm*4.6mm*5μm, Aglient) chromatographic column, Spherisorb ODS2 (250mm*4.6mm*5μm, waters) chromatographic column, Prodigy ODS3 (250mm*4.6mm*5μm, Phenomenex) chromatographic column on Dendrobium nobile phenols and flavonoids The influence of the separation effect of the chromatographic components of the class components. Such as figure 1 The shown Spherisorb ODS2 (150mm*4.6mm*5μm, waters) chromatographic column, the Spherisorb ODS2 column peak quantity, peak height, peak shape are all good but the peak density, the resolution is far from the experimental requirements, so the final experiment A chromatographic column of the type 150mm*4.6mm*5μm is not used. The 250mm*4.6mm*5μm type chromatogra...

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Abstract

The invention discloses a dendrobe chromatogram finger print measuring method. The method comprises the following steps of: (1) cutting medical dendrobe, drying at low temperature, crushing, sieving for later use; (2) putting a proper amount of samples into a conical bottle, adding a proper amount of monohydric alcohol, performing ultrasonic treatment for 20 minutes, filtering, sprinkling filter slag by using the monohydric alcohol multiple times, combining filter liquors, and volatizing the filter liquors to near dryness; (3) dissolving by using the monohydric alcohol, fixing the volume, filtering by using a microporous filter membrane of 0.45 mum to be used as a sample solution; and (4) precisely sucking a comparison solution and the sample solution respectively, injecting into a liquid chromatograph for analyzing. The invention can obtain the chromatogram map conveniently and rapidly, and has easy obtaining of the raw materials, simple technology and advanced technique. According to the literature records, the variety of dendrobium plants used as the medical dendrobe is approximate to 100. The invention controls the product quality by using multiple components and multiple indexes, and has more reliable results, easy obtaining of the raw materials and simple technology compared with the quality control indexes.

Description

【Technical field】 [0001] The present invention relates to a chromatographic fingerprinting technique to formulate a chromatographic fingerprinting method for the chemical components containing phenols and flavonoids extracted from medicinal dendrobium, so as to facilitate the quality assurance of each step in raw material procurement and product production process. to monitor. 【Background technique】 [0002] Dendrobium is a commonly used precious Chinese medicinal material. It has been recorded in successive herbal medicines and various pharmacopoeias. Scholars at home and abroad have studied and explored the chemical components of various Dendrobium, and found that the chemical components contained in this genus are diverse, including many Sugars, alkaloids, flavonoids, quinones, sesquiterpenes, coumarins and steroidal glycosides. In recent years, many scholars have carried out pharmacological activity research and clinical application on the components isolated from vario...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 成金乐李先霞徐吉银梁逸曾易伦朝
Owner ZHONGSHAN ZHONGZHI PHARMA GRP
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