Dendrobe chromatogram finger print measuring method

A fingerprint and determination method technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve the effect of fast analysis speed, reliable results, and easy access to raw materials
CN101726547AActive Publication Date: 2010-06-09ZHONGSHAN ZHONGZHI PHARMA GRP
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
ZHONGSHAN ZHONGZHI PHARMA GRP
Publication Date
2010-06-09

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Abstract

The invention discloses a dendrobe chromatogram finger print measuring method. The method comprises the following steps of: (1) cutting medical dendrobe, drying at low temperature, crushing, sieving for later use; (2) putting a proper amount of samples into a conical bottle, adding a proper amount of monohydric alcohol, performing ultrasonic treatment for 20 minutes, filtering, sprinkling filter slag by using the monohydric alcohol multiple times, combining filter liquors, and volatizing the filter liquors to near dryness; (3) dissolving by using the monohydric alcohol, fixing the volume, filtering by using a microporous filter membrane of 0.45 mum to be used as a sample solution; and (4) precisely sucking a comparison solution and the sample solution respectively, injecting into a liquid chromatograph for analyzing. The invention can obtain the chromatogram map conveniently and rapidly, and has easy obtaining of the raw materials, simple technology and advanced technique. According to the literature records, the variety of dendrobium plants used as the medical dendrobe is approximate to 100. The invention controls the product quality by using multiple components and multiple indexes, and has more reliable results, easy obtaining of the raw materials and simple technology compared with the quality control indexes.
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Description

【Technical field】

[0001] The present invention relates to a chromatographic fingerprinting technique to formulate a chromatographic fingerprinting method for the chemical components containing phenols and flavonoids extracted from medicinal dendrobium, so as to facilitate the quality assurance of each step in raw material procurement and product production process. to monitor. 【Background technique】

[0002] Dendrobium is a commonly used precious Chinese medicinal material. It has been recorded in successive herbal medicines and various pharmacopoeias. Scholars at home and abroad have studied and explored the chemical components of various Dendrobium, and found that the chemical components contained in this genus are diverse, including many Sugars, alkaloids, flavonoids, quinones, sesquiterpenes, coumarins and steroidal glycosides. In recent years, many scholars have carried out pharmacological activity research and clinical application on the components isolated from vario...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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