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EM bacteria cultured by ascophyllum nodosum enzymatic hydrolysate and application of EM bacteria to prevention and treatment of meloidogyne

A technology for enzymatic hydrolysis solution of Ascophyllum nodosum and leaf algae enzymatic hydrolysis solution is applied in the field of EM bacteria, and can solve the problems of difficult damage of chemical pesticides, increased types of harmful crops, limited varieties of crops with resistance to root-knot nematodes, etc.

Active Publication Date: 2021-11-30
山东科大创业生物有限公司
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Problems solved by technology

After being harmed by it, the quality of crops will decline, and the yield will be greatly reduced, or even lost. At the same time, the occurrence of nematodes will intensify the damage of soil-borne diseases such as fusarium wilt, yellow water chestnut and blight, resulting in a decline in plant resistance. Infected by other pathogens
[0005] At present, the prevention and control measures of root-knot nematode in my country mainly include agricultural control, physical control, chemical control and biological control, but chemical control is still the main method, and the comprehensive level of control measures is low, resulting in the accumulation of drug resistance of root-knot nematodes. and other problems make the situation of root-knot nematode control more severe
[0006] In the method of controlling such diseases, because root-knot nematodes usually live in the soil and in the roots of plants, it is difficult for general chemical pesticides to control their harm. Therefore, this type of diseases has become the object of frequent use of a large number of highly toxic pesticides, which has a great impact on the production of agricultural products. Security poses a serious threat
At the same time, since chemical nematicides can only control the number of nematodes in a short period of time, there are still problems such as high cost, short duration of effect and drug resistance.
In addition, although some traditional control methods, such as crop rotation and planting disease-resistant varieties, can play a certain role in control, there are not many crops that can be rotated in actual production, and the crop varieties that are resistant to root-knot nematodes are very limited.
Therefore, traditional control methods cannot solve this type of disease
To sum up, due to the current lack of practical, efficient and sustainable control technology, the occurrence area of ​​root-knot nematode disease continues to expand, and the types of harmful crops continue to increase, resulting in severe challenges for agricultural production. Nematicidal biopesticides have very broad application value and market prospects

Method used

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  • EM bacteria cultured by ascophyllum nodosum enzymatic hydrolysate and application of EM bacteria to prevention and treatment of meloidogyne
  • EM bacteria cultured by ascophyllum nodosum enzymatic hydrolysate and application of EM bacteria to prevention and treatment of meloidogyne
  • EM bacteria cultured by ascophyllum nodosum enzymatic hydrolysate and application of EM bacteria to prevention and treatment of meloidogyne

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1. Enzymolysis of Ascophyllum nodosum

[0027] Divided into three groups A, B, and C according to different operating conditions, and carried out enzymatic hydrolysis of Ascophyllum nodosum, as follows.

[0028] Group A:

[0029] The fresh Ascophyllum nodosum was mechanically ground into a homogenate according to the material-to-water ratio of 1:2, added at 45°C according to the ratio of homogenate: ADL compound enzyme = 100:0.5, fully stirred and reacted for 28 hours, and passed through a vibrating sieve with a 100-mesh coarse The Ascophyllum nodosum enzymatic solution was obtained by filtration, and the viscosity of the Ascophyllum nodosum enzymatic solution was measured to be 79mPa s (39.5°C).

[0030] Group B:

[0031] The fresh Ascophyllum nodosum was mechanically ground into a homogenate according to the material-to-water ratio of 1:2, added at 50°C according to the ratio of homogenate: ADL compound enzyme = 100:0.4, fully stirred and reacted for 24 h...

Embodiment 2

[0034] Embodiment 2: EM bacteria liquid fermentation

[0035] According to the same operation process as follows, the A group, B group, and C group of the obtained Ascophyllum nodosum enzymolyzate were used to carry out three fermentation tests in parallel respectively, and correspondingly obtained EM bacteria fermentation liquid finished product 1, EM bacteria fermentation liquid finished product 2, Finished product of EM bacteria fermentation broth3.

[0036] (1) Inoculate EM strains in the seed liquid fermentation medium at a weight ratio of 5%, and ferment at 35°C at a shaking speed of 200r / min for 48 hours to obtain a seed liquid fermentation liquid;

[0037] The invention adopts the preparation method of the seed liquid fermentation liquid medium: 2% sucrose, 0.5% urea, 1.2% molasses, 0.5% sodium dihydrogen phosphate, 0.25% calcium carbonate, supplemented with deionized water to 100%, and sterilized at 121°C for 20 minutes;

[0038] (2) Inoculate the seed fermentation l...

Embodiment 3

[0044] Embodiment 3: the field test biocontrol effect of EM bacteria fermented liquid finished product on tomato

[0045] Using the tomatoes grown in Zouping Changshan’s severe root-knot nematode test field in previous years, the following experimental design was carried out to verify its control effect on tomato root-knot nematode (hereinafter, the finished product of EM bacteria fermentation liquid is also referred to as “biocontrol agent”):

[0046] Treatment 1: blank control (no drug application)

[0047] Treatment 2: Comparative example 2kg / mu

[0048] Treatment 3: Biocontrol agent 2kg / mu

[0049] Treatment 4: biocontrol agent 4kg / mu

[0050] Treatment 5: biocontrol agent 6kg / mu

[0051] After the tomatoes were harvested, the roots of the tomato plants were dug out, and surveyed according to the root-knot nematode classification survey indicators, as follows: grade 0 (no root knots); grade 1 (less than 5 root knots in the whole plant); 6-15 root knots); 3rd grade (16-...

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Abstract

The invention relates to the technical field of microorganisms, and particularly provides ascophyllum nodosum enzymatic hydrolysate, a process for culturing EM bacteria by using the ascophyllum nodosum enzymatic hydrolysate as a culture medium component, and an application of the cultured EM bacteria to prevention and treatment of meloidogyne. The ascophyllum nodosum enzymatic hydrolysate is obtained by performing enzymolysis on ascophyllum nodosum by using ADL compound enzyme, and can be used as an important component to be added into a culture medium for culturing the EM bacteria, so that stable and high-yield viable bacteria can be obtained. Research on obtained fermentation liquid shows that the fermentation liquid has an extremely remarkable prevention and control effect on M.incognita, a new direction is provided for prevention and control of the meloidogyne, and the fermentation liquid better serves agricultural production.

Description

technical field [0001] The invention relates to obtaining EM bacteria by fermenting and culturing the Ascophyllum nodosum enzymatic solution, and using the obtained EM bacteria to control root-knot nematodes. Background technique [0002] Among the four major pathogens that cause plant infectious diseases, plant parasitic nematodes are more harmful than bacteria and viruses, and are second only to fungal diseases. Plant nematode disease is an important disease in the world's agricultural production, and it causes losses of up to 100 billion U.S. dollars to more than 21 major crops in the world every year. Among them, root-knot nematode (Meloidogyne spp.) is the most harmful type of plant parasitic nematode. More than 50% of the losses are caused by crops, and the annual economic losses to the world's important economic crops are as high as tens of billions of dollars. [0003] Root-knot nematode has a wide range of hosts and can infect more than 3,000 types of plants, inclu...

Claims

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Application Information

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IPC IPC(8): C12N1/38C12N1/20C12N1/16A01N63/20A01N63/32A01P5/00
Inventor 宋晓军孙志锋
Owner 山东科大创业生物有限公司
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