Preparation method and application of bacillus methylotrophilus LW-6 bacterial agent for biological control of meloidogyne

A methylotrophic, bacillus-based technology, applied to chemicals for biological control, microbial-based methods, biocides, etc., can solve unsatisfactory effects, reduced quality of tobacco leaves, root-knot nematode hazards, etc. problems, to achieve the effects of strong environmental adaptability and environmental friendliness, strong fixed value and competitive ability, and simple and easy preparation method

Pending Publication Date: 2020-06-02
陕西恒田生物农业有限公司
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

In my country, with the expansion of tobacco serial planting area and the prolongation of continuous cropping time, soil-borne root-knot nematode damage is becoming more and more serious, which has become one of the main limiting factors of tobacco production, resulting in reduced tobacco yield and quality.
At present, chemical control, crop rotation and disease-resistant varieties are mostly used in the prevention and control of root-knot nematode disease, but the effect is not ideal, and there are many disadvantages
For example, the chemical nematicides currently used are mostly highly toxic or highly toxic and high-residue pesticides, which cause serious pollution or damage to soil microorganisms, plants, water sources and the atmosphere. Residues in plants will indirectly cause harm to humans

Method used

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  • Preparation method and application of bacillus methylotrophilus LW-6 bacterial agent for biological control of meloidogyne

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Preparation of a methylotrophic bacillus LW-6 bacterial agent for biological control of root-knot nematode:

[0025] (1) plate activation and optimal cultivation: the methylotrophic bacillus LW-6 stock slant is inoculated on the plate medium for activation, and the composition of the plate medium and its percentage by weight are: peptone 3.5%, yeast extract 1.3% , NaCl 1.5%, agar powder 5.7%, glucose 0.9%, sodium carbonate 0.1%, magnesium sulfate 0.1%, iron sulfate 0.1%, the balance is water, pH is 7.2~7.3, culture conditions are: at a temperature of 32 ℃ Under culture for 80h.

[0026] (2) Seed culture: inoculate the activated plate bacteria into the preferred liquid medium, and cultivate it for 80 hours at a temperature of 32° C. to obtain a fermented seed liquid; wherein the composition and weight percentage of the liquid medium are: peptone 3.5%, yeast extract 1.4%, NaCl 1.8%, glucose 00.9%, sodium carbonate 0.1%, magnesium sulfate 0.1%, iron sulfate 0.1%, the bala...

Embodiment 2

[0031] Indoor biological control effect of methylotrophic Bacillus LW-6 bacterial agent on root-knot nematode The isolation of root-knot nematode was carried out by the Baemann funnel method, and the second instar larvae of root-knot nematode were obtained. The isolated root-knot nematodes were added to a 500-fold dilution of the liquid preparation of methylotrophic Bacillus LW-6 bacteria, and the same amount of water was used as a control, and the nematodes were left at room temperature for 1 hour to observe the number of surviving and dead nematodes. The results showed that, in the control, the mortality rate of the second instar larvae of root-knot nematode was 4.41%, while the mortality rate of the second instar larvae of root-knot nematode treated with the 500-fold dilution of the liquid preparation of methylotrophic bacillus LW-6 reached 93%, so it can be seen that the liquid preparation with methylotrophic bacillus LW-6 as the active ingredient has a strong biological co...

Embodiment 3

[0033] Control effect of methylotrophic Bacillus LW-6 bacterial agent on tobacco root-knot nematode in field plots. The variety of flue-cured tobacco is Honghua Dajinyuan. Add 500ml of water to 1 gram of vegetative Bacillus sp. LW-6 to irrigate the roots, and use the clear water without the preparation as a control. 60 days after transplanting, the disease index of tobacco root-knot nematode disease was investigated by digging roots. During the transplanting period, the treatment group was repeatedly treated twice, and the control group was repeatedly treated with clean water. The disease index of tobacco root-knot nematode treated with the methylotrophic bacillus LW-6 bacterial agent was 20.3, while the disease index of the blank control without applying the preparation reached 69.5. It can be seen that the methylotrophic bacillus LW- The biological control effect of the preparation with 6 as the active ingredient on tobacco root-knot nematode disease is 70.79%.

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Abstract

The invention discloses a preparation method and application of a bacillus methylotrophilus LW-6 bacterial agent for biological control of meloidogyne. The bacillus methylotrophilus LW-6 has the advantages of strong stress resistance, high-temperature resistance, drought resistance, and fast growth and reproduction, and has the characteristics of strong colonization and competitiveness, an obviousnematicidal effect, no toxicity to humans and animals and environmental friendliness. The viable spores and secondary metabolites of the bacillus methylotrophilus LW-6 have the functions of preventing and controlling tobacco meloidogyne and tobacco growth promotion, the efficiency of preventing and controlling the meloidogyne by drip irrigation treatment can reach 70.79%, and the meloidogyne killing ratio can reach 93%. The number of viable spores of a liquid microbial agent prepared by the steps of plate-activated culture of the bacillus methylotrophilus LW-6, seed culture and fermentation can be up to 5 billion / ml, and the preparation method specially comprises the following steps: preferred plate culture, specific seed culture, preferred fermentation, ultrafiltration concentration, low-temperature drying, and dosage form processing preparation.

Description

technical field [0001] The invention relates to the preparation and application of methylotrophic bacillus LW-6 for biological control of root-knot nematodes, and belongs to the technical field of microorganisms or biological control. Background technique [0002] Tobacco is an important economic crop, and its growth process is harmed by many diseases. Tobacco root-knot nematode is the main soil-borne disease, which causes tobacco yield reduction or failure. Root-knot nematode (Meloidogyne spp.) is a class of endoparasitic nematodes without strict host specificity, can infect a variety of crops, seriously endanger agricultural production, and cause global losses of tens of billions of dollars every year. Root-knot nematodes are widely distributed all over the world and have a wide range of hosts. In addition to direct damage, they can also spread bacterial and fungal diseases and cause greater losses of many commercial crops. In my country, with the expansion of tobacco ser...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/22A01P5/00A01P21/00C12R1/07
CPCC12N1/20C12R2001/07C12N1/205
Inventor 唐少然安航唐满仓安德荣马玉明
Owner 陕西恒田生物农业有限公司
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