Compositional main pesticide containing destruxins of Metarhizium anisopliae and hexaflumuron
A technology of metarhizalin and hexaflumuron, which is applied in biocides, biocides, animal repellents, etc., can solve the problems of decreased control effect, achieve high larvicidal activity, significant insecticidal effect, and insecticidal broad spectrum effect
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Embodiment 1
[0016] Example 1 Toxicity determination of main insecticidal agent of the present invention to cruciferous vegetable pests
[0017] The diamondback moth (Plutella xylostella) was used as the experimental object, and the leaf drug film method was used for indoor toxicity determination. Metarhizine and hexaflumuron were mixed in different proportions, with 6 concentration gradients for each treatment, and 3 repetitions for each concentration. The metarhizine is any one of metarhizin A, metarhizin B or metarhizin E or a mixture of two or more in any proportion.
[0018] Clean and non-pesticide-polluted pakchoi leaves were dipped in the medicinal solution for 5 seconds, then taken out and dried in the shade, put into a petri dish with a diameter of 9 cm and covered with filter paper, and then inserted into 15 third-instar larvae of diamondback moth. Placed in a breeding room for feeding. The feeding conditions are temperature 25-28° C., RH 70%-80%, and light as L:D=14h:10h. 72h...
Embodiment 2
[0022] Embodiment 2: Indoor toxicity determination of metarhizine, hexaflumuron single agent and main agent of the present invention to Spodoptera litura
[0023] When measuring indoor toxicity, take appropriate amount of metarhizine and hexaflumuron respectively, make a certain concentration of mother liquor with acetone and place it in the refrigerator (4°C) for standby. . Each treatment was replicated 3 times, and each replicate had 1 petri dish, and 15 third-instar Spodoptera litura larvae were placed in each petri dish. First, cut the leaves of cabbage into small pieces of 6-7 cm, soak them in the medicinal solution for about 5 seconds, take them out, dry them indoors, put the leaves into a petri dish connected with Spodoptera litura, and use wet cotton balls to moisturize. Change the cabbage leaves every 24 hours after the medicine and clean the petri dishes. The experiment was repeated twice. Observe and record the survival of Spodoptera litura 24, 48, and 72 hours a...
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