Plant expression carrier construction for divalent insect-resistant gene and transgenic plant acquiring method thereof

A technology for expressing vectors and genes, which is applied to the construction of plant expression vectors for bivalent insect-resistant genes and the acquisition of transgenic plants. The effect of resistance
CN101161816BInactive Publication Date: 2011-01-26BEIJING CHANGLE ERSHENG GENE TECH
0 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN ยท China
Patent Type
Patents(China)
Current Assignee / Owner
BEIJING CHANGLE ERSHENG GENE TECH
Publication Date
2011-01-26
Estimated Expiration
Not applicable ยท inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention relates to an Amaranthus retroflexus agglutinin (ARA) gene derived from Amaranthusr etroflexus L. and the gene sequence of gene sequence-Bt of Bacillus thuringiensis (Bt) parasporal crystal protein, wherein, both genes are built into the same plant expression vector; moreover, the plant expression vector of divalent insect-resistant gene is transferred to plant. In addition, the invention provides the method for obtaining transgenic plant of the genes.
Need to check novelty before this filing date? Find Prior Art

Description

field of invention

[0001] The present invention relates to a double amaranth agglutinin (ARA) gene derived from Amaranthus retroflexus L. and Bt (Bacillus thuringiesis, Bt for short) gene sequence derived from Bacillus thuringiensis parasporal crystal protein. Construction of plant expression vector for insect-resistant gene and method for obtaining transgenic plants. In the present invention, by cloning the nucleotide sequence of a retroverted amaranthus agglutinin gene (ARA) and the artificially synthesized Bt insecticidal protein gene, the two insecticidal genes are constructed into the same plant expression vector, and the phloem-specific The expression promoter (BSP) drives the specific expression of the ARA gene in the phloem, and the cauliflower mosaic virus (CaMV) 35S promoter drives the constitutive expression of the Bt gene in plants. It has good poisonous effect on Lepidoptera and Lepidoptera insects, and the increase of insect resistance to insecticidal protein ca...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More