Purpureocillium lilacinum capable of preventing and treating Meloidogyne and application thereof

A technology of violacea lilac, strains, applied to lilac and its application in agricultural production, can solve the problem of single action object, lack of efficient strains, physiological characteristics and functional characteristics of lilac strains differences, etc.

Active Publication Date: 2020-11-27
MOON (GUANGZHOU) BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, there are great differences in the physiological and functional characteristics of P. lilacinus strains from different sources, and there is a lack of high-efficiency strains that are truly efficient, widely used, and suitable for industrial production.
For example, the patent CN102851219A discloses a wild-type Paecilomyces lilacinus E16 strain isolated from banana plants with recurrent nematode disease. This bacterial strain is only highly pathogenic to root-knot nematode, and has a single target
Another example is disclosed in the patent CN107058120A a strain of P. lilacinus P136-1-1 bacterial strain through ultraviolet mutagenesis, which can efficiently kill root-knot nematode cucumber, but the sporulation ability of this bacterial strain is not high (5.9 high 10 8 spores / dish, 20mL / dish), and it has non-directionality after ultraviolet mutagenesis treatment, which is difficult to meet the needs of industrial production

Method used

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  • Purpureocillium lilacinum capable of preventing and treating Meloidogyne and application thereof
  • Purpureocillium lilacinum capable of preventing and treating Meloidogyne and application thereof
  • Purpureocillium lilacinum capable of preventing and treating Meloidogyne and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1: Separation of P. lilacinus MN12228

[0022] P. lilacinus MN12228 was isolated from wheat field soil in Xi'an, Shaanxi Province, and was preserved in the General Microorganism Center (CGMCC) of China Committee for Microbial Culture Collection (CGMCC) on July 12, 2016. The address is Beichen West Road 1, Chaoyang District, Beijing. No. 3 Beijing Institute of Microbiology, the preservation number is CGMCC 12773. The specific separation method is as follows: 10 g of soil sample was weighed, added to 90 mL of sterilized water containing 0.1% Tween, and incubated in a shake flask at 28° C. for 1 hr. The culture solution was diluted step by step with water containing 0.1% Tween into different gradients, and then spread on the PDA solid medium (200 grams of potatoes, 20 grams of glucose, 15-20 grams of agar, 1000 ml of distilled water), at 28 ° C In a constant temperature incubator for 4 days, pick a single colony after 4 days, continue to inoculate it on the PDA...

Embodiment 2

[0027] Embodiment 2: the spore-producing ability of P. lilacinus, the determination of the number of viable bacteria

[0028] Determination of sporulation ability in laboratory:

[0029] Inoculate activated P. lilacinum MN12228 in PDA medium, place it in an incubator at 25-28°C for 7 days, wash out the spores on the medium with sterile water, make a spore suspension, and use a hemocytometer The counting plate was counted, and the spore concentration was determined to be 9.7×10 9 Spores / mL, higher than the sporulation ability (5.9 × 10 8 spores / dish, 20mL / dish).

[0030] Determination of sporulation production of cultures in pilot plant:

[0031] Mix wheat bran, ammonium sulfate and sucrose into a solid preparation according to the weight ratio of 85:10:5, put it into a solid fermenter, then add 20% pure water (by total weight) and mix evenly, at a high temperature of 121°C and under high pressure conditions Sterilize for 20 minutes. After cooling, inoculate activated P. l...

Embodiment 3

[0035] Embodiment 3: the preparation of P. lilacinum MN12228 inoculum

[0036] The P. lilacinus inoculum consists of the following components by weight: 8-12 parts of culture of P. lilacinum MN12228, 5-9 parts of dispersant NNO, 2-6 parts of SDS, and 3-5 parts of pull-open powder , Potassium dihydrogen phosphate 6-10 parts, make up to 100 parts with carrier diatomaceous earth. The spore content in the prepared P. lilacinus inoculum is not less than 10 8 A / g, preferably 10 9 pcs / g.

[0037] Table 1: The content of each component of the bacterial agent

[0038] serial number MN12228 NNO SDS pull open powder Potassium dihydrogen phosphate diatomite Group I 8 5 2 3 6 76 Group II 10 7 4 4 8 67 Group III 12 9 6 5 10 58

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Abstract

The invention provides an isolated Purpureocillium lilacinum strain, a composition containing the same, and an application of the strain in prevention and treatment of Meloidogyne. The strain is highin sporulation capacity, high in viable count and good in prevention and treatment effect, and can be used for prevention and treatment of various plants.

Description

technical field [0001] The present application relates to the field of microbial control, and more specifically relates to P. lilacinus and its application in agricultural production. Background technique [0002] Root-knot nematodes (RKN, Latin name Meloidogyne) have a wide range of hosts and can infect more than 3,000 species of plants in 114 families, including monocotyledonous, dicotyledonous and herbaceous plants. Pests can cause crop yield reduction of 10% to 20%, and can reach more than 75% in severe cases, and the annual economic loss is estimated to be about 118 billion US dollars. RKN forms root knots on the roots of plants, preventing water and nutrient uptake and infecting wounds that facilitate infection by pathogenic microorganisms. Meloidogyne incognita infects the roots of almost all cultivated plants and is one of the most important RKN species. [0003] At present, chemical control, crop rotation and resistance breeding are mainly used to control RKN. Ho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/30A01P5/00C12R1/645
CPCA01N63/30C12R2001/645C12N1/145
Inventor 王科晶余昕彤曹鸿一王琳
Owner MOON (GUANGZHOU) BIOTECH CO LTD
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