Application of benzoic acid in prevention and treatment of plant nematode diseases
A technology of plant nematodes and benzoic acid, applied in the fields of application, nematicides, plant growth regulators, etc., can solve the problems of low biohazard, tremor paralysis, unconsciousness, etc.
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Embodiment 1
[0037] Embodiment 1: Determination of the poisonous activity and egg hatching inhibitory activity of benzoic acid to root-knot nematode, cyst nematode and pine wood nematode
[0038] 1. Toxic activity and egg hatching inhibitory activity assay of benzoic acid to root-knot nematode, the method is as follows:
[0039] S1.1. Sample configuration of the solution to be tested:
[0040] Take 0.05g of benzoic acid and add 200μL of acetone to dissolve, add 10mL of sterile distilled water, pass through filter membranes with a pore size of 0.45μm and 0.22μm, and make a 5mg / mL sample of the solution to be tested, and then dilute the samples of the solution to be tested to the desired concentration. The concentration needs to be tested to obtain a series of different samples of the solution to be tested.
[0041] S1.2, preparation of second-instar larvae suspensions of root-knot nematode incognita and root-knot nematode javanica:
[0042] Rinse the root-knot nematode diseased roots of f...
Embodiment 2
[0070] Embodiment 2: Pot control test of benzoic acid and abamectin to root-knot nematode of spinach
[0071] 1. The experimental method is carried out through the following steps:
[0072] Mix the substrate soil well and put it into pots (diameter 7cm×height 7cm), each pot is filled with 250mL of soil, and the convolvulus seedlings (4-5 cotyledons) are transplanted into the pots, and the root irrigation treatment is carried out for the first time after one week of transplanting the seedlings, and each pot is watered. Drug 120mL (benzoic acid: 0.3mg / mL, 0.5mg / mL, 1.0mg / mL; abamectin: 0.05mg / mL), each group set 4 repetitions, with acetone and water as the control group, after 2 days each 360 second-instar larvae of Meloidogyne incognita and 480 eggs were potted, and the second root irrigation treatment was carried out 4 days after inoculation, with 80 mL of pesticide per pot, and the third root irrigation treatment was carried out 10 days after inoculation. Pouring 100mL, taki...
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