2results about How to "Significant lethal effect" patented technology

The application discloses a dsRNA targeting phosphoenolpyruvate carboxykinase gene PEPCK and application of the dsRNA in prevention and control of spider mites, and relates to the technical field of genetic engineering. The technical points are as follows: the nucleotide sequence of the dsRNA targeting phosphoenolpyruvate carboxykinase gene PEPCK is shown in SEQ ID NO:1, and the dsRNA can be used for preventing and controlling spider mites or preparing products for preventing and controlling spider mites. The dsRNA targeting phosphoenolpyruvate carboxykinase gene PEPCK provided in the application has a high lethal effect on female adult spider mites, can effectively control the population number of spider mites, and reduces the field damage of spider mites. The dsRNA can be used for development of transgenic mite-resistant plants or novel mite-killing agents, can be used for targeted resistance to specific types of mites, does not affect other beneficial insects and organisms, and can improve the mite resistance of plants.

The application discloses a kind of real fly luring and killing agents.The real fly luring and killing agent is composed of the following components by mass percentage: 75-85% of apple snail meat hydrolysate, 3-7% of pest toxic components, 5-15% of auxiliary materials and 2-5% of stabilizers.The apple snail meat hydrolysate is a mixture of apple snail meat after protease hydrolysis and enzyme inactivation, and the pest toxic components are Metarhizium or Beauveria.The luring and killing agent uses the protein hydrolysate of invasive species apple snail meat as the main raw material to prepare food lure, and then adds a small amount of Metarhizium / Beauveria to prepare the luring and killing agent.The synergistic effect of food lure and Metarhizium / Beauveria significantly improves the luring and killing effect on real flies.The new real fly luring and killing agent has the characteristics of high efficiency and green environmental protection.