The invention discloses a taxol-carried nano microbubble and a preparation method thereof. The microbubble has a membrane consisting of a lipoid bilayer and containing N2; the lipoid bilayer comprises hydrophilic groups and hydrophobic groups; the hydrophilic groups are bonded with taxol and the taxol is carried in the membrane consisting of the lipoid bilayer; and the lipoid bilayer is formed by compounding lecithin, cholesterol, polyethylene glycol (PEG)-4000, glucan, Span 80, methanol, ether, 3 percent human serum albumin, a taxol standard substance, and phosphate buffered saline (PBS) liquid. The taxol-carried nano microbubble does not have toxicity or side effect, can be used for peripheral intravenous injection, can pass through the lung and a capillary bed of the whole body (the diameter is generally less than 5 um), can be circulated to tissues of the whole body along with blood after the intravenous injection, has high stability, has a long duration in blood circulation, anddoes not influence blood flow dynamic of the whole body or a certain system.