The application provides an injectable microparticulate acellular
dermal matrix and a preparation method and application thereof, and belongs to the technical field of preparation of acellular dermal matrixes.The preparation method of the microparticulate acellular
dermal matrix comprises the following steps: (1) taking a dermal layer, performing homogenization and
defatting treatment, and then performing
decellularization treatment; (2) freezing under the condition of
liquid nitrogen at-196 DEG C for 1-2 minutes; (3) placing in a
cold trap circulating medium, controlling the temperature to be below-50 DEG C, and performing ball milling under the temperature condition by using milling balls with different sizes; (4) after the ball milling is completed, screening the obtained product in step (3), and directly collecting the product in a sterile collection container by using a
turbine airflow to form a low-pressure area and storing the product, so that the microparticulate acellular
dermal matrix is obtained.The microparticulate acellular dermal matrix obtained by the method has good uniformity, high product quality and high stability, and has good injectability, and can be used for minimally invasive
surgical treatment of primary premature ejaculation.