The invention discloses a spectrophotometer for batch detection, and a detection method thereof, and belongs to the field of detection equipment. The spectrophotometer comprises light path systems, wherein the plurality of light path systems which are arranged in a straight line are respectively provided with a conveying belt and a bench, a plurality of cuvettes are arranged on the conveying beltand the bench at equal intervals by taking the light path system as a symmetry axis, a liquid injection station and a plurality of detection stations are arranged above the conveying belt, a dumping station, a cleaning station, a drying station and a preparation station are arranged above the conveying belt, a liquid injector is arranged above the liquid injection station, and a liquid collectingdisc, a cleaner and a dryer are arranged below the dumping station, the cleaning station and the drying station respectively. According to the invention, the touch frequency of the cuvette can be reduced or eliminated, the accuracy of the wavelength of monochromatic light can be improved, the residual components of detection liquid on the cuvette can be reduced, the detection speed can be increased, the detection cost can be reduced, and the accuracy of the detection result can be improved.