70 results about "Cellular signal transduction" patented technology

The present invention relates generally to methods for stimulating cells, and more particularly, to a novel method to concentrate and stimulate cells that maximizes stimulation and / or proliferation of such cells. In the various embodiments, cells are stimulated and concentrated with a surface yielding enhanced proliferation, cell signal transduction, and / or cell surface moiety aggregation. In certain aspects methods for stimulating a population of cells such as T-cells, by simultaneous concentration and cell surface moiety ligation are provided by contacting the population of cells with a surface, that has attached thereto one or more agents that ligate a cell surface moiety and applying a force that predominantly drives cell concentration and cell surface moiety ligation, thereby inducing cell stimulation, cell surface moiety aggregation, and / or receptor signaling enhancement. Also provided are methods for producing phenotypically tailored cells, including T-cells for the use in diagnostics, drug discovery, and the treatment of a variety of indications, including cancer, viral infection, and immune related disorders. Compositions of cells having specific phenotypic properties produced by these processes are further provided.

The present invention relates generally to methods for stimulating cells, and more particularly, to a novel method to concentrate and / or stimulate cells that maximizes stimulation and / or proliferation of such cells. In the various embodiments, cells are stimulated and concentrated with a surface yielding enhanced proliferation, cell signal transduction, and / or cell surface moiety aggregation. In certain aspects methods for stimulating a population of cells such as T-cells, by simultaneous concentration and cell surface moiety ligation are provided by contacting the population of cells with a surface, that has attached thereto one or more agents that ligate a cell surface moiety and applying a force that predominantly drives cell concentration and cell surface moiety ligation, thereby inducing cell stimulation, cell surface moiety aggregation, and / or receptor signaling enhancement. Also provided are methods for producing phenotypically tailored cells, including T-cells for the use in diagnostics, drug discovery, and the treatment of a variety of indications, including cancer, viral infection, and immune related disorders. Compositions of cells having specific phenotypic properties produced by these processes are further provided.

The present invention relates to novel cytoplasmic tyrosine kinases isolated from megakaryocytes (megakaryocyte kinases or MKKs) which are involved in cellular signal transduction pathways and to the use of these novel proteins in the diagnosis and treatment of disease. The present invention further relates to specific megakaryocyte kinases, designated MKK1, MKK2 and MKK3, and their use as diagnostic and therapeutic agents.

A method for conducting a broad range of biochemical analyses or manipulations on a series of nano- to subnanoliter reaction volumes and an apparatus for carrying out the same are disclosed. The invention is implemented on a fluidic microchip to provide high serial throughput. In particular, the disclosed device is a microfabricated channel device that can manipulate nanoliter or subnanoliter reaction volumes in a controlled manner to produce results at rates of 1 to 10 Hz per channel. The reaction volumes are manipulated in serial fashion analogous to a digital shift register. The invention has application to such problems as screening molecular or cellular targets using single beads from split-synthesis combinatorial libraries, screening single cells for RNA or protein expression, genetic diagnostic screening at the single cell level, or performing single cell signal transduction studies.

A liquid micronutirent elicitor involving a cell to cell signal transduction system within a plant which is applied to propagules (seed and / or plant) causes natural defensive responses to be produced by the seed and / or plant. This substance may exist as a seed coating, irrigation water, and / or foliar spray for a period of time so that the propagules may have enhanced disease control until the propagule develops sufficiently to fend for itself against parasitic nematodes. The benefits of incorporating the solution include increased vigor, blooms, and harvests. Crops include legumes including soybeans, as well as wheat, canola, corn, peanuts, sunflowers, peppers, tomatoes, grapes, and potatoes.

The present invention relates to kinase modulators of the naphthyridine type and to the preparation and use thereof as medicaments for the modulation of misdirected cellular signal transduction processes, in particular for influencing the function of tyrosine and serine / threonine kinases and for the treatment of malignant or benign tumours and other disorders based on pathological cell proliferation, such as, for example, restenosis, psoriasis, arteriosclerosis and cirrhosis of the liver.

The invention provides a method for preparing a quantum dot immune fluorescent probe. A monoclonal antibody fluorescent probe marked by a water-soluble quantum dot is covalently connected with a monoclonal antibody at the tail end of a carboxylation-modified quantum dot; and generally, each quantum dot can be connected with one or more antibody molecules. The preparation method comprises the following steps of: firstly, preparing a mercaptopropanoic acid-modified quantum dot in a water solution by using a hydrothemal method; and then connecting the carboxylation-modified quantum dot with antibody protein by using a covalent coupling method to obtain the quantum dot immune fluorescent probe with favorable fluorescent characteristic and excellent bioactivity. Compared with the prior art, the invention has the advantages that the probe has favorable dispersibility, strong specificity, high fluorescent intensity and photobleaching resistance and can be applied to the fields of immunological detection, cellular signal transduction, fluorescent imaging technologies of biological cells or tissues and other relevant bio-medical researches. The method is simple and easy to apply and can be finished in a general biological and chemical laboratory.

The invention relates to a fluorescent probe for detecting superoxide anions (O2-) and sulfane sulfur, in particular to a cyanine compound and application thereof. The cyanine compound is as shown in general formula I and serves as the fluorescent probe for hydrogen sulfide. In the presence of the superoxide anions (O2-) and sulfane sulfur, the corresponding fluorescence-emission wavelength and strength of the fluorescent probe vary, and the fluorescent probe can be used for detecting the superoxide anions (O2-) and sulfane sulfur, can greatly reduce the interference of external detection conditions and is high in detection signal-to-noise ratio and good in sensitivity and selectivity. The compound serving as the fluorescent probe can be used for linkage detection of the level of the superoxide anions (O2-) and sulfane sulfur in complex biological samples and is of great biomedicine significance for studying cellular signal transduction of the superoxide anions (O2-) and sulfane sulfur. Formula I.

The invention relates to the use preferably of at least one active ingredient for the prophylaxis and / or therapy of a viral disease, wherein this active ingredient inhibits at least one component of the cellular signal transduction pathway for the activation of the transcription factor NF-kB such that the virus multiplication is inhibited. The present invention relates furthermore to the local, preferably aerogenic administration of the active ingredient according to the invention for inhibiting a virus multiplication. The active ingredient according to the invention may be combined with at least one further antivirally effective substance for the prophylaxis and / or therapy of a viral disease.

The present invention makes available a rapid, reproducible, robust assay system for screening and identifying pharmaceutically effective compounds that specifically interact with and modulate the activity of a cellular protein, e.g., a receptor or ion channel. The subject assay enables rapid screening of large numbers of compounds to identify those which act as an agonist or antagonist to the bioactivity of the cellular protein. In this system, the cell is treated with a compound, and functional interaction of this compound with a cellular receptor yields a detectable signal, which can be specifically measured. The subject assays include methods of identifying compounds which specifically modulate, for example, heterologous receptors coupled to the pheromone response pathway in yeast. The subject assays are particularly amenable to the identification of specific agonists and antagonists of G protein-coupled receptors.

An assay device for determining the presence of analytes in a cell lysate comprises a porous support member and a plurality of binding reagents arranged and immobilized at multiple reaction sites on the support member. The binding reagents are selected and arranged to assess the status of a selected cellular signal transduction pathway / protein-protein interactive network. In a further aspect, a method for assessing the status of a signal transduction pathway comprises generating a lysate of cells, the lysate retaining one or more pathway molecules present in one or more states and the pathway molecules reflecting signal transduction events taking place in the cells. The method further includes applying the lysate to an immobilized series of binding reagents which can discriminate the pathway molecules and their states. Binding events between the pathway molecules and the binding reagents are identified and the state of the selected signal pathway is determined.

The present invention relates to a benzothiadiazole compound represented by the following formula I, and a preparation method and use thereof, the benzothiadiazole compound has the biological activityof inhibiting protein-tyrosine-phosphatase SHP2, can be used as a tool compound to study the biological functional relevance of the protein-tyrosine-phosphatase SHP2 in a cell signal transduction process, and provides a new means for prevention and treatment of cancer and metabolic and immune diseases.

The present invention makes available a rapid, reproducible, robust assay system for screening and identifying pharmaceutically effective compounds that specifically interact with and modulate the activity of a cellular protein, e.g., a receptor or ion channel. The subject assay enables rapid screening of large numbers of compounds to identify those which act as an agonist or antagonist to the bioactivity of the cellular protein. In particular, the assay of the invention makes use of a cell that harbors a protein that is responsive to a cellular signal transduction pathway. The protein is operatively linked to a polypeptide which causes a detectable signal to be generated upon stimulation of the pathway, e.g., when a compound interacts with and modulates the activity of a cellular receptor or ion channel of the cell. Thus, the cell provides a signal generation means comprising a novel fusion protein the expression of which is independent of stimulation / activation of the signal transduction pathway, but the activity of which is responsive to the signal transduction pathway.

The present invention is directed to compounds having the structure (I) wherein R1, R2, R3, R4, R5 and m are as defined herein. The compounds of this invention are novel therapeutic agents for the treatment of cancer, metabolic diseases and skin disorders in mammalian subjects. These compounds are also useful modulators of gene expression. They exert their activity by interfering with certain cellular signal transduction cascades. The compounds of the invention are thus also useful for regulating cell differentiation and cell cycle processes that are controlled or regulated by various hormones or cytokines. In particular, the invention relates to compounds that induce apoptosis of cancer cells and therefore may be used for the treatment or prevention of cancer, including advanced cancers and pre-cancerous cells. The invention also discloses pharmaceutical compositions and methods of treatment of disease in mammals.

The invention provides plant miticide prepared by taking scopoletin lactone as the main raw material and then adding in solvent, solubilizer and / or emulsifier, is environmental-friendly harmonious pesticide which is safety to human beings and animals, and has profound significance in the long-termed development of the ecological environment, the health of human beings and the integrated control of diseases. Compared with traditional pesticide, the scopoletin lactone plant miticide is characterized in and has the advantages that: 1. the scopoletin lactone exists in nature is a large amount, has low toxicity, is easy to degrade and is safety to the ecological environment; 2. the scopoletin lactone extraction method is simpler; 3. the scopoletin lactone can be also synthesized manually, has low cost and is easily applied to industrial large-scale production; 4. the scopoletin lactone can be solved in various solvent, is easy to be mixed with other anti-mite components to prepare medicine, and can play the role of improving the anti-mite effect; and 5. the scopoletin lactone can inhibit cellular signal transduction, is good neuromuscular blocking agent, and can quickly palsies harmful mites.

The present invention makes available a rapid, reproducible, robust assay system for screening and identifying pharmaceutically effective compounds that specifically interact with and modulate the activity of a cellular protein, e.g., a receptor. or ion channel. The subject assay enables rapid screening of large numbers of compounds to identify those which act as an agonist or antagonist to the bioactivity of the cellular protein. In this system, the first cell is treated with a compound, and functional interaction of this compound with a cellular receptor yields a secreted signal. A second cell, bearing a receptor for this secreted signal, makes use of an indicator gene in a signaling pathway coupled to this second receptor. The subject assays include methods of identifying compounds which specifically modulate, for example, heterologous receptors coupled to the pheromone response pathway in yeast. The subject assays are particularly amenable to the identification of specific agonists and antagonists of G protein-coupled receptors.

The invention relates to the field of biological medicines, in particular to a chimeric antigen receptor specifically optimized according to NK cell signal transduction characteristics and an application thereof. The chimeric antigen receptor scFv is composed of a light chain variable region and a heavy chain variable region and connected to an NKp44 transmembrane region through a CD8 hinge structure, and an intracellular segment activation signal transduction structural domain is composed of a CD3 zeta ITAM motif, a 2B4 activation motif, a DNAM1 activation motif and a DAP10 activation motif which are connected in series. The chimeric antigen receptor is used for modifying natural killer (NK) cells, and the modified NK cells (CAR-NK) can be used for treating tumors with positive tumor specific antigens. In a killing test, compared with a classic third-generation T-CAR structure, the structure has the advantages that the killing capability of the NK cells on tumor cells is obviously enhanced, and good anti-tumor activity is shown in an in-vivo model.

The invention relates to pyrido[2,3-b]pyrazine compounds of formulae (Ia) and (Ib) which have activity to the modulation of misdirected cellular signal transduction processes such as tyrosine kinases, serine / threonine kinases and / or lipid kinases, their preparation and use as medicaments, especially for the treatment of malignant disorders and other disorders based on pathological cell proliferations:

The present invention makes available a rapid, reproducible, robust assay system for screening and identifying pharmaceutically effective compounds that specifically interact with and modulate the activity of a cellular protein, e.g., a receptor or ion channel. The subject assay enables rapid screening of large numbers of compounds to identify those which act as an agonist or antagonist to the bioactivity of the cellular protein. In this system, the cell is treated with a compound, and functional interaction of this compound with a cellular receptor yields a detectable signal, which can be specifically measured. The subject assays include methods of identifying compounds which specifically modulate, for example, heterologous receptors coupled to the pheromone response pathway in yeast. The subject assays are particularly amenable to the identification of specific agonists and antagonists of G protein-coupled receptors.

The present invention is directed to compounds having the structure Formula (I) wherein R1, R2, R3, R4, R5 and m are as defined herein. The compounds of this invention are novel therapeutic agents for the treatment of cancer, metabolic diseases and skin disorders in mammalian subjects. These compounds are also useful modulators of gene expression. They exert their activity by interfering with certain cellular signal transduction cascades. The compounds of the invention are thus also useful for regulating cell differentiation and cell cycle processes that are controlled or regulated by various hormones or cytokines. In particular, the invention relates to compounds that induce apoptosis of cancer cells and therefore may be used for the treatment or prevention of cancer, including advanced cancers and pre-cancerous cells. The invention also discloses pharmaceutical compositions and methods of treatment of disease in mammals.

The invention relates to a phenyl ring-aromatic ring cascaded micro-molecular organic compound which can be used as a protein-tyrosine-phosphatase subtype inhibitor and is shown in a general formula I as described in the specification. The compound can be used as a tool compound for research on the biological functions of a variety of subtypes of a protein-tyrosine-phosphatase family in cell signal transduction, so novel means is provided for prevention and treatment of cancers, metabolism and immunological diseases, cardiovascular diseases and nervous diseases. The invention also relates to a preparation method and medicinal application of the compound.

The invention relates to a method for preparing novel germplasm of S gene homozygote by radioactive mutagenesis of pollen, which belongs to the field of molecular genetics. Seven S1 gene homozygote plants, four S2 gene homozygote plants, three S3 gene homozygote plants and two S5 gene homozygote plants are screened and appraised together from self-bred progeny plants by radiating huanghua and fengshui pear flower pollen with Co60 gamma 40Gy mutagenesis radiation. The plants of S gene homozygote provide good test piece for future researches on ploidy culturing of pear, cell signal transduction which identifies pollen and pistil mutually, time and space expression characteristics of S gene and function verification of protein product thereof, guide the field pollination and have good theoretical research value and production application.

The invention is about the extracting method for the protoplast of the pear flower powder and the pollen tube. The process includes: first to collect the ripe pollen, culture in the pollen medium for 3-3.5h, then to enzymolysis for 50min and centrifugate. So the active protoplast would be get. It can be as the material for the studying of the physiplogical-biochemical characteristic and the cell signal transduction of the pollen and the pollen tube.

The invention relates to fluorescent probes used for detecting superoxide anions (O2.<->) and bivalent mercury ions (Hg<2+>), in particular to fluorophore derivatives and application thereof. For example, the cyanine fluorophore derivatives have a chemical structure as shown in a structural formula I, and the cyanine compounds are taken as fluorescent probes for the O2.<-> and the Hg<2+>. The fluorescent probes provided by the invention have the corresponding fluorescence emission wavelength and intensity change in the presence of the O2.<-> and the Hg<2+>, can be used for detecting the O2.<-> and the Hg<2+>, can greatly reduce the interference of external detection conditions, and is high in detection signal-to-noise ratio and good in sensitivity and selectivity. As the fluorescent probes, the compounds can be used for detecting the levels of the O2.<-> and the Hg<2+> in complex biological samples and have important biomedical significance for the study of cell signal transduction of the O2.<-> and the Hg<2+>. The structural formula I is described in the description.

The invention discloses an oxygen-containing five-membered heterocyclic compound, a synthesis method, a pharmaceutical composition and application thereof, and belongs to the technical field of medicines and preparation and application thereof. The oxygen-containing five-membered heterocycle has the biological activity of inhibiting the protein tyrosine phosphatase SHP2, can be used as a tool compound for researching the biological function relevance of the protein tyrosine phosphatase SHP2 in the cell signal transduction process, and provides a new means for preventing and treating cancers and metabolic and immune diseases.

The present invention relates to pyridylpyrimidine derivatives of the general formula (I): wherein R represents hydrogen or methyl and Z represents nitrogen containing functional groups, the use of the pyridylpyrimidine derivatives as pharmaceutically active agents, especially for the prophylaxis and / or treatment of prion infections and prion diseases, as well as compositions containing at least one pyridylpyrimidine derivative and / or pharmaceutically acceptable salt thereof. Furthermore, the present invention is directed to methods for preventing and / or treating prion infections and prion diseases using said pyridylpyrimidine derivatives. Human cellular protein kinases, phosphatases and cellular signal transduction molecules are disclosed as targets for detecting, preventing and / or treating prion infections and diseases, especially BSE, vCJD, or CJD which can be inhibited by the inventive pyridylpyrimidine derivatives.

The present invention is directed to novel compounds according to formulae wherein R1, R2, R3 and R4 are as defined herein. The invention also discloses methods of preparation, pharmaceutical compositions, and methods of disease treatment utilizing pharmaceutical compositions comprising these compounds. The compounds of this invention are novel therapeutic agents for the treatment of cancer, diabetes, metabolic diseases and skin disorders in mammalian subjects. These compounds are also useful modulators of gene expression. They exert their activity by interfering with certain cellular signal transduction cascades. The compounds of the invention are thus also useful for regulating cell differentiation and cell cycle processes that are controlled or regulated by various hormones or cytokines. The invention also discloses pharmaceutical compositions and methods of treatment of disease in mammals.

The present invention relates generally to methods for stimulating, activating, and maintaining or increasing the polyclonality of expressed TCRs in a population of T cells. In the various embodiments, cells are stimulated with a surface, wherein the surface has attached thereto one or more agents that ligate a cell surface moiety of at least a portion of the T cells and stimulates at least a portion of the T cells, yielding enhanced proliferation, cell signal transduction, and / or cell surface moiety aggregation. In certain aspects methods for stimulating a population of cells such as T-cells, by cell surface moiety ligation are provided by contacting the population of cells with a surface, that has attached thereto one or more agents that ligate a cell surface moiety thereby inducing cell stimulation, cell surface moiety aggregation, and / or receptor signaling enhancement. Also provided are methods for producing T-cells for the use in diagnostics and the treatment of a variety of indications, including cancer, viral infection, and immune related disorders. Compositions of cells having increased polyclonality produced by these processes are further provided.

The invention discloses the application of berberine in medicament for treating hepatitis B virus infection. In the invention, adopting an HepG2.2.15 cell model for the in vitro selection of anti-hepatitis B virus medicine, preliminarily selecting the effect of the medicament by measuring the difference of HBeAg and HBsAg expression volumn in supernatant fluid of the cell after administration, andfurther determining the effectiveness of the medicine by studying the inhibitory effect of the HBV DNA reproduction level (ccc DNA) in the HepG2.2.15 cell by the medicament. Meanwhile, the influenceon the reproduction of the hepatitis B virus by the transcriptional level of the medicine is studied according to the activity inhibitory effect to HBV viral promoters by the medicine, and key HBV cis-regulatory elements sensitive to the antivirus medicine are found. A pivotal cellular signal transduction pathway possibly participating the transcription reproduction for inhibiting viruses by the medicine is analyzed and verified on the basis, the antivirus molecular mechanism of the medicament is further studied, and the medicament is indicated to be developed and applied to clinical treatmentas an anti-hepatitis B effective medicament.

The invention relates to a fluorescence probe, in particular to a cyanine-based organic compound, an intermediate and application thereof. The structural formula of the cyanine-based organic compoundis as shown in FORMULA I. The compound is used as the fluorescence probe for detecting O2.- and H2Sn. According to the fluorescence probe disclosed by the invention, under the existence of O2.- and H2Sn, a corresponding fluorescence emission wavelength and corresponding intensity changes, so as to detect O2.- and H2Sn; furthermore, the interference of external detection conditions can be greatly reduced, and the detection precision is improved. The compound used as the fluorescence probe can be used for detecting O2.- and H2Sn levels inside and outside cells and transducing cell signals for deeply researching O2.- and H2Sn, and particularly has an important biomedical significance in researches on physiological and pathological effects of H2Sn in living cells.