Method for preparing quantum dot immune fluorescent probe

A technology of immunofluorescence and quantum dots, which is applied in the fields of nanomaterials, bioanalytical chemistry, and nanobiomedicine, and can solve the problem of unreliable combination

Inactive Publication Date: 2010-12-08
ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The combination of quantum dots and antibodies can be directly combined with antibodies by electrostatic adsorption. Although this method is simple and feasible, the combination is not reliable enough. Changes in environmental factors such as pH, temperature, and ionic strength can uncouple quantum dot-antibody complexes.

Method used

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  • Method for preparing quantum dot immune fluorescent probe
  • Method for preparing quantum dot immune fluorescent probe
  • Method for preparing quantum dot immune fluorescent probe

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Embodiment 1

[0016] Add 0.4g of NaBH4 and 0.08g of tellurium powder to a 50mL conical flask, add 5mL of deionized water, pass nitrogen for 20min, immediately plug the soft rubber stopper, insert a syringe needle, and magnetically stir for 2h to obtain a milky white freshly prepared NaHTe solution . Take 0.5mL of CdCl with a concentration of 200mmol / L 2 Aqueous solution, 0.5 mL of freshly prepared mercaptopropionic acid aqueous solution with a concentration of 200 mmol / L was added into another 50 mL conical flask, 14 mL of deionized water was added, and NaOH solution with a concentration of 1 mol / L was added dropwise to adjust the pH to 12.0. Nitrogen was passed through the solution for 20min, immediately plug the soft rubber stopper, insert a syringe needle, use a 1mL syringe to extract 0.2mL of the freshly prepared NaHTe solution, quickly inject it into the conical flask containing the Cd precursor solution, and put the conical flask into 96 Heat in a ℃ water bath for 1 h, take it out an...

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Abstract

The invention provides a method for preparing a quantum dot immune fluorescent probe. A monoclonal antibody fluorescent probe marked by a water-soluble quantum dot is covalently connected with a monoclonal antibody at the tail end of a carboxylation-modified quantum dot; and generally, each quantum dot can be connected with one or more antibody molecules. The preparation method comprises the following steps of: firstly, preparing a mercaptopropanoic acid-modified quantum dot in a water solution by using a hydrothemal method; and then connecting the carboxylation-modified quantum dot with antibody protein by using a covalent coupling method to obtain the quantum dot immune fluorescent probe with favorable fluorescent characteristic and excellent bioactivity. Compared with the prior art, the invention has the advantages that the probe has favorable dispersibility, strong specificity, high fluorescent intensity and photobleaching resistance and can be applied to the fields of immunological detection, cellular signal transduction, fluorescent imaging technologies of biological cells or tissues and other relevant bio-medical researches. The method is simple and easy to apply and can be finished in a general biological and chemical laboratory.

Description

technical field [0001] The invention relates to a preparation method of a quantum dot immunofluorescence probe, belonging to the fields of nanomaterials, bioanalytical chemistry and nanobiomedicine. Background technique [0002] Quantum dots (quantum dots, QDs), also known as semiconductor nanocrystals, whose radius is less than or close to the exciton Bohr radius, are mainly composed of II-VI group elements (such as CdTe, CdSe) or III-V group elements (such as GaAs, InP )constitute. Due to their unique optical and electrical properties, quantum dots have attracted extensive attention in the scientific community in recent years. Their broad and continuous absorption spectrum in the ultraviolet-visible light range, and narrow and symmetrical fluorescence emission spectrum make them have single excitation and multiple emission. Multicolor marker function. Quantum dots also have the unique properties of good photostability and resistance to photobleaching, which make them pro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/536
Inventor 牛婉婷张路遥潘敏陈裕泉
Owner ZHEJIANG UNIV
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