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Method for preparing novel germplasm of S gene homozygote by radioactive mutagenesis of pollen

A homozygous, new germplasm technology, applied in the field of molecular genetics, can solve problems such as interference and inability to clarify, and achieve the effects of simple operation, saving production costs, and accurate and fast molecular marker identification methods

Inactive Publication Date: 2010-09-22
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

However, there are two different S gene loci in pear cultivars, that is, there are two pollen S genes and two style S genes. There will be mutual interference when proteins specifically recognize functions, and it is impossible to figure out which gene is responsible

Method used

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  • Method for preparing novel germplasm of S gene homozygote by radioactive mutagenesis of pollen
  • Method for preparing novel germplasm of S gene homozygote by radioactive mutagenesis of pollen
  • Method for preparing novel germplasm of S gene homozygote by radioactive mutagenesis of pollen

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Embodiment Construction

[0027] (1) Experimental method

[0028] 1. The tested variety 'Huanghua' (Huanghua, S 1 S 2 ) and ‘Fengshui’ (Hosui, S 3 S 5 ) are the main cultivars in production (China Pear Online, http: / / www.pear.org.cn / ).

[0029] 2. Select the species whose growth is 5-6 years old, collect the young leaves of each species, and store them in liquid nitrogen for later use. Collect flower buds 1 day before flowering or on the same day, take out the anthers, place them on sulfuric acid paper, loosen the powder naturally in a dry place, put them into airtight bottles, and store them in a -20°C freezer for later use.

[0030] The collected 'Huanghua' and 'Fengshui' pollen were treated with Co 60 γ-ray radiation treatment, radiation dose 40Gy, radiation time 15min, each repeated twice. On the same day of treatment, the emasculated 'Huanghua' and 'Fengshui' were self-pollinated, each with 100 inflorescences, and then covered with sulfuric acid paper bags. 25 days after pollination, invest...

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Abstract

The invention relates to a method for preparing novel germplasm of S gene homozygote by radioactive mutagenesis of pollen, which belongs to the field of molecular genetics. Seven S1 gene homozygote plants, four S2 gene homozygote plants, three S3 gene homozygote plants and two S5 gene homozygote plants are screened and appraised together from self-bred progeny plants by radiating huanghua and fengshui pear flower pollen with Co60 gamma 40Gy mutagenesis radiation. The plants of S gene homozygote provide good test piece for future researches on ploidy culturing of pear, cell signal transduction which identifies pollen and pistil mutually, time and space expression characteristics of S gene and function verification of protein product thereof, guide the field pollination and have good theoretical research value and production application.

Description

1. Technical field [0001] The invention relates to a method for creating a new pear S gene homozygous germplasm by pollen radiation mutagenesis, and belongs to the field of molecular genetics. 2. Background technology [0002] Pear is one of the main fruits in my country and even in the world. The existing cultivated area in my country is 1,087,400 hectares (data from China Agricultural Yearbook in 2007). Bearing fruit, pollination trees must be rationally configured to ensure fruit setting in production. Because of this, there is no gene homozygous variety (individual) in the genus Pear. In addition, in the genus Pyrus, there is also the phenomenon of cross-pollination incompatibility between varieties, so there is a certain blindness in the allocation of pollinated varieties in the field. The research so far has shown that pear self-pollination infertility and cross-pollination infertility between cultivars are controlled by the S gene. If there is mutual pollination, it ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/06A01H1/02C12Q1/68
Inventor 张绍铃齐永杰吴华清吴俊陶书田齐开杰
Owner NANJING AGRICULTURAL UNIVERSITY
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