Method for extracting pear pollen protoplast by enzymolysis

A technology of protoplasts and extraction methods, applied in the fields of biochemistry and food science, can solve the problems of pollen protoplasts losing biological vitality, inability to obtain pollen protoplasts, and inability to be suitable for plant pollen, etc., to achieve easy operation and practical application, and simple structure , the effect of developmental synchronization

Inactive Publication Date: 2006-08-16
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

Although there are these methods for removing the wall, there are many problems in these methods, and the main problems have: one is that the pollen protoplasts obtained by these methods not only lose their original biological vitality, but also bring certain nutrients to the nutrients.

Method used

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  • Method for extracting pear pollen protoplast by enzymolysis
  • Method for extracting pear pollen protoplast by enzymolysis
  • Method for extracting pear pollen protoplast by enzymolysis

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Embodiment Construction

[0037] Extracting plant pollen (tube) protoplasts is one of the necessary prerequisites for studying the physiological and biochemical characteristics and functions of pollen (tube). We applied the method of the present invention to obtain vigorous pear pollen tube protoplasts, and used patch clamp to directly identify the calcium channels in the pollen tube, the calcium gradient in the pollen tube and its related functions, in order to broaden and in-depth study the electrical power of the pollen tube. The chemical properties lay the foundation. As we all know, obtaining pro-pollen (tube) protoplasts is a prerequisite for conducting patch clamp experimental research. So far, the root hairs, pseudoroots and hyphae of algae have been laser cut off the cell wall. This method requires more expensive experimental equipment. Secondly, at present, the protoplasts that are active and intact with pollen are all made of young and fresh pollen. However, there is no report on pollen that can...

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Abstract

The invention is about the extracting method for the protoplast of the pear flower powder and the pollen tube. The process includes: first to collect the ripe pollen, culture in the pollen medium for 3-3.5h, then to enzymolysis for 50min and centrifugate. So the active protoplast would be get. It can be as the material for the studying of the physiplogical-biochemical characteristic and the cell signal transduction of the pollen and the pollen tube.

Description

Technical field [0001] The invention is a method for extracting pear pollen and pollen tube protoplasts, relates to a method for breaking plant cell walls and obtaining viable protoplasts, and belongs to the fields of biochemistry and food science. Background technique [0002] Plant pollen (pollen) not only plays an extremely important role in plant proliferation, expansion of genetic composition and distribution range, but also extremely rich in nutrients. Recent research shows that pollen contains a wide variety of carbohydrates, organic acids, proteins, nucleic acids, enzymes and coenzymes, pigments, vitamins and minerals and other nutrients. Among them, the protein content is very rich, reaching 7%-40%, which is better than casein. Pollen contains 18 kinds of amino acids, including 8 kinds of amino acids that are necessary for the human body, and its content is 5-7 times more than milk and eggs. In particular, the content of lysine is particularly rich, which is beneficial t...

Claims

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Application Information

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IPC IPC(8): C12N5/04
Inventor 张绍铃屈海泳吴俊
Owner NANJING AGRICULTURAL UNIVERSITY
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