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Method for extracting protoplast from pear flower powder by citric acid

A technology of protoplast and citric acid, which is applied in the fields of biochemistry and food science, can solve the problems of pollen protoplast losing biological vitality, unable to obtain pollen protoplast, unable to be suitable for plant pollen, etc., and achieves easy operation and practical application and simple structure. , the effect of developmental synchronization

Inactive Publication Date: 2006-07-12
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

Although there are these methods for removing the wall, there are many problems in these methods, and the main problems have: one is that the pollen protoplasts obtained by these methods not only lose their original biological vitality, but also bring certain nutrients to the nutrients. The destructiveness that reduces to a great extent the nutritive value that has; The 2nd is that these methods that obtain pollen protoplast cannot be suitable for all plant pollen, such as, can't obtain the pollen of Rosaceae fruit tree such as pear, apple with these methods Protoplast

Method used

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  • Method for extracting protoplast from pear flower powder by citric acid
  • Method for extracting protoplast from pear flower powder by citric acid

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Embodiment Construction

[0023] Extracting plant pollen (tube) protoplasts is one of the necessary prerequisites for studying the physiological, biochemical characteristics and functions of pollen (tube). We applied the method of the present invention to obtain active pear pollen tube protoplasts, and used patch clamp to directly identify the calcium channel in the pollen tube, the calcium gradient in the pollen tube and its related functions. Chemical characterization lays the groundwork. As we all know, obtaining pollen (tube) protoplasts is the premise of carrying out patch clamp experimental research, but so far, laser cutting of cell walls is used in root hairs, algae rhizomes and hyphae. This method requires relatively expensive experimental equipment. At present, the active and complete protoplasts for obtaining pollen are all young and fresh pollen as materials, but there is no report for mature pollen that can only be harvested once a year, such as pears, apples, peaches, etc. . There is no...

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Abstract

The invention relates to a method for extracting protoplast from pear pollen and pollen tube, relating to method of breaking plant cell wall and getting protoplast, belonging to biochemistry and foodstuff field; which mainly applying the property of pollen cell wall to get intact pollen or pollen tube protoplast with original activeness. The invention employs method of removing pollen wall with citric acid, which is to add dry pollen into the citric acid solution of 2-3mol / l, culturing for about 30 minutes, the pollen protoplast getting out of the sprout, centrifuging and getting pollen protoplast with wall removed, the protoplast got from this method is characterized by the retaining nutriment, no pollution, simple process, low cost and suited to be applied in foodstuff field.

Description

technical field [0001] The invention relates to a method for extracting pear pollen and pollen tube protoplasts, relates to a method for breaking plant cells and obtaining active protoplasts, and belongs to the fields of biochemistry and food science. Background technique [0002] Plant pollen (pollen) not only plays an extremely important role in plant reproduction, expansion of genetic composition and distribution range, but also has extremely rich nutrition. In recent years, studies have shown that pollen contains a wide variety of carbohydrates, organic acids, proteins, nucleic acids, enzymes and coenzymes, pigments, vitamins and minerals and other nutrients. Among them, the protein content is very rich, reaching 7%-40%, which is better than casein. Pollen contains 18 kinds of amino acids, including 8 kinds of amino acids necessary for the human body. Its content is 5-7 times more than that of milk and eggs, especially the content of lysine is particularly rich, which i...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/076A23L21/20
Inventor 张绍铃屈海泳吴俊
Owner NANJING AGRICULTURAL UNIVERSITY
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