Use of active ingredients for the prophylaxis and/or therapy of viral diseases

a technology of active ingredients and viral diseases, applied in the field of test systems, can solve the problems of large number of fatalities, huge economic cost factor, and substantial threat to the health of man and animal, and achieve the effect of reducing the risk of infection

Inactive Publication Date: 2006-11-02
INAMED GMBH INST FUR AEROSOLMEDIZIN
View PDF2 Cites 14 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Infections by RNA or DNA viruses are a substantial threat for the health of man an animal.
Infections by influenza viruses still belong to the big epidemics of mankind and cause year for year a large number of fatalities.
They are an immense cost factor for the economy, for instance by inability to work because of illness.
The problem of controlling in particular RNA viruses is the adaptability of the viruses caused by a high fault rate of the viral polymerases, thus the preparation of suitable vaccines as well as the design of antiviral substances being very difficult.
The hopes placed on this therapeutic agent thus could not be fulfilled.
Due to their in most cases very small genomes and

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

example 1

Test System for Identifying Antiviral Active Ingredients

[0047] By means of retroviral transduction of the human lung epithelial cells A549, the canine kidney epithelial cells MDCK and the monkey cells Vero, cell lines were prepared, which stably express either a dominant-negative form of the IKK (IKK KD) or a dominant-interfering mutated form of the inhibitor of NF-kB, mIkB. Furthermore, corresponding lines were also generated, which express an active form of the IKK (IKK EE). The constructs as well as their efficiency with regard to the NF-kB mediated gene expression in cells were already described (Denk et al., J Biol Chem 276, 28451-28458, 2001).

[0048] For generating the stable cell lines, the respective cDNAs for IKK KD, mIkB and IKK EE were cloned in sense orientation into the retroviral expression vector pCFG5 IEGZ (Kuss et al., Eur J Immunol 29, 3077-3088, 1999). Beside the messenger RNA of the ‘gene of interest’, the vector DNA also codes for the messenger RNA of the “gree...

example 2

Inhibition of the Viral NF-kB Activation and Reduction of the Influenza Viruses In Vitro

2.1: Acetylsalicylic Acid (ASA).

[0052] Salicylates, such as ASA or sulfasalazines are widely clinically used as pain alleviating and inflammatory agents. Newer publications show that these substances are direct and effective inhibitors of the IKK (Yin et al., Nature 396, 77-80, 1998; Weber et al., Gastroenterology 119, 1209-1218, 2000) and can inhibit in vitro the multiplication of influenza viruses (Huang and Dietsch, New Engl J Med 319, 797, 1988). Thus, as a positive control, ASA was used. Lung epithelial cells A549 were treated with increasing concentrations of ASA in the range from 0.01 mM-5 mM. These concentrations remained in the culture medium during the full experiment. One hour after treatment the infection by the influenza A virus strain fowl plague virus (FPV) with a multiplicity of the infection of 1 (M.O.I.=1) was performed. Another 24 h after the infection, the titers of the new...

example 3

Effect of ASA on the Influenza Infection in the Mouse

3.1 IP / Parenteral Administration.

[0057] C57 Bl / 6 mice were nasally infected by 5,000 pfu / 20 μl influenza virus (fowl plague virus, FPV). 30 min before the infection, 500 μl ASA (50 mM=9 mg / ml PBS, Sigma-Aldrich Steinheim) were i.p. injected, then ASA (50 mM=9 mg / ml) was administered continuously in drinking water. For control purposes, PBS was injected or administered. Body weight, death rate and survival time were determined. 30 mice per group were treated.

[0058] The following results were obtained. Whereas in the control group, all animals died of influenza, in the ASA group approx. 20% of the animals survived the infection. Further, the average survival time of the dead animals was clearly longer than in the control group. ASA in concentrations of 50 mM, however, led to a distinct reduction of the body weight because of the toxic dose.

3.2 Aerogenic Administration.

[0059] C57 Bl / 6 mice were nasally infected by 5,000 pfu / 20...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Massaaaaaaaaaa
Massaaaaaaaaaa
Molar densityaaaaaaaaaa
Login to view more

Abstract

The invention relates to the use preferably of at least one active ingredient for the prophylaxis and/or therapy of a viral disease, wherein this active ingredient inhibits at least one component of the cellular signal transduction pathway for the activation of the transcription factor NF-kB such that the virus multiplication is inhibited. The present invention relates furthermore to the local, preferably aerogenic administration of the active ingredient according to the invention for inhibiting a virus multiplication. The active ingredient according to the invention may be combined with at least one further antivirally effective substance for the prophylaxis and/or therapy of a viral disease.

Description

FIELD OF THE INVENTION [0001] The invention relates to a test system for identifying active ingredients, which are suitable for the prophylaxis and / or treatment of viral diseases, the uses of such active ingredients for preparing a pharmaceutical composition for the prophylaxis and / or treatment of viral diseases, formulations for such pharmaceutical compositions and methods for preparing such pharmaceutical compositions. PRIOR ART AND BACKGROUND OF THE INVENTION [0002] Infections by RNA or DNA viruses are a substantial threat for the health of man an animal. To the RNA viruses belong the negative-strand-RNA viruses, such as for instance influenza viruses or the Borna disease virus. Infections by influenza viruses still belong to the big epidemics of mankind and cause year for year a large number of fatalities. They are an immense cost factor for the economy, for instance by inability to work because of illness. Of substantial economic importance are also infections by the Borna dise...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K38/16A61K31/7056A61K31/7048A61K31/63A61K31/60A61K31/56A61K31/401A61K31/13A61K31/355A61K31/353A61K31/00A61K31/19A61K31/335A61K31/35A61K31/40A61K31/54A61K31/70A61K36/82A61K45/06A61P31/12A61P31/16G01N33/50
CPCA61K31/00A61K31/13G01N33/5041A61K31/19A61K31/335A61K31/35A61K31/40A61K31/54A61K31/60A61K31/70A61K36/82A61K45/06G01N33/5008A61K2300/00
Inventor PLANZ, OLIVERPLESCHKA, STEPHANSEDLACEK, HANS-HARALDLUDWIG, STEPHAN
Owner INAMED GMBH INST FUR AEROSOLMEDIZIN
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap