The object of the present invention is to provide
DNA and
RNA designed on the basis of the sequence of an
enzyme gene for repressing the expression of the
enzyme gene generating a lachrymatory factor from a precursor of the lachrymatory factor, and also a vector required for introducing
DNA for repressing the expression of the
gene of the lachrymatory factor-producing
enzyme into a vegetable. The present invention relates to
DNA comprising at least one sequence selected from the following sequences and a
regulatory sequence connected to said sequence so as to make the transcription possible: (a)
a DNA sequence encoding a
protein or a polypeptide of a lachrymatory factor-producing enzyme or a part of the DNA sequence in a
sense orientation, an
antisense orientation or both the orientations, wherein the
protein or the polypeptide has an effect of converting 1-propenylsulfenic acid into the lachrymatory factor; (b) a
regulatory sequence of
a DNA in a vegetable
genome determined on the basis of the
DNA encoding the
protein or the polypeptide of the lachrymatory factor-producing enzyme or a part of the
regulatory sequence in a
sense orientation, an
antisense orientation or both the orientations; and (c)
a DNA sequence located between the
DNA encoding the protein or the polypeptide of the lachrymatory factor-producing enzyme and the regulatory sequence of the DNA in the vegetable
genome DNA determined on the basis of the
DNA encoding the protein or the polypeptide of the lachrymatory factor-producing enzyme or a part of the DNA sequence in a
sense orientation, an
antisense orientation or both the orientations.