27 results about "Thaumatin" patented technology

The invention discloses a construction method and application of a chlamydomonas reinhardtii-sourced high-sweetness protein variant engineering algal strain, the construction method comprises the following steps: carrying out gene modification on sweeteners Thaumatin and Brazzein, optimizing preference codons of chlamydomonas reinhardtii to synthesize CrThaumatin and CrBrazzein encoding genes, cloning the encoding genes into an expression vector pGM6, introducing the recombinant expression vector into a chlamydomonas reinhardtii wild-type chlamydomonas sp. Algal strain, and carrying out high-sweetness protein variant engineering algal strain of the chlamydomonas reinhardtii-sourced high-sweetness protein variant engineering algal strain of the chlamydomonas reinhardtii. And screening by using a paromomycin resistance marker carried by the expression vector to successfully obtain an engineering algal strain. According to the present invention, the engineering strain is subjected to fermentation production to obtain the dry and stable Chlamydomonas reinhardtii powder rich in the target sweet protein, and the protein purification oral test results show that the sweet taste of the Chlamydomonas reinhardtii powder is 3-5 times of the sweet taste of the natural Thaumatin and Brazzein protein;

To provide a garlic flavor enhancer, a garlic-component-containing food or beverage that contains the enhancer, and a method for enhancing garlic flavor in a garlic-component-containing food or beverage.SOLUTION: A garlic flavor enhancer is used which contains at least one selected from the group consisting of a Luo Han Guo extract and thaumatin, or contains a Luo Han Guo extract and a stevia extract.SELECTED DRAWING: None

The preparation method comprises the following steps: dissolving antioxidant dextrin and ferulic acid in deionized water to form a mixed solution, adjusting the pH value, adding transglucosidase, carrying out a reaction, carrying out enzyme deactivation, and carrying out freeze drying to obtain hydrophobic modified resistant dextrin; respectively dissolving the obtained hydrophobic modified resistant dextrin and thaumatin in a citric acid-sodium citrate buffer solution to form mother liquor, respectively adjusting the pH of the mother liquor, adding NaCl to adjust proper ionic strength, and slowly mixing the two blended mother liquor under magnetic stirring; after mixing, allowing the solution to stand at room temperature; according to the invention, unique characteristics of thaumatin and resistant dextrin are creatively and synergistically utilized, and a dual blood glucose reducing mechanism combining front-end resistance control and rear-end regulation is formed.

The application discloses a composition containing burdock polysaccharide for reducing blood sugar of type 2 diabetes patients and a preparation method and application thereof, and belongs to the technical field of medicine and food. The composition contains burdock polysaccharide, chitooligosaccharide, chlorogenic acid, epicatechin, resveratrol, ginsenoside Rd, and vitamin D, vitamin B1, vitamin B6, vitamin B12, magnesium glycinate and thaumatin protein. The application takes burdock polysaccharide as a core long-acting component, takes chitooligosaccharide as a short-acting synergistic component, constructs a long-short-acting synergistic blood sugar reducing system, and realizes the organic combination of rapid blood sugar reduction and stable blood sugar control. Experiments show that the composition can significantly reduce the fasting blood sugar of type 2 diabetes model rats, improve insulin resistance and glucose tolerance, reduce blood lipid and inflammatory factor levels, and reduce oxidative stress damage. The alpha-glucosidase inhibitory activity and in-vivo comprehensive blood sugar reducing effect of the composition are better than those of single burdock polysaccharide, and the composition has good taste and is suitable for long-term use of diabetes patients.

The present application relates to a kind of urea[ 13 C] reagent, by weight parts, urea[ 13 C] reagent includes urea[ 13 C] 10-75 parts;Citric acid 800-2500 parts;Mannitol 500-3000 parts;And flavoring agent 20-50 parts;Flavoring agent includes at least one of stevioside, sucralose, neotame and thaumatin.The present application reduces the amount of mannitol, without the need for a large amount of mannitol, to meet the quality requirements of product, reduce the risk of patients using drug, product appearance and content are stable and no impurities in normal temperature storage environment, product can be continuously maintained for 6 months in high temperature and high humidity environment.

This invention discloses a method for constructing an engineered algal strain of high-intensity sweet protein variants derived from *Chlamydomonas reinhardtii* and its application. First, the sweeteners Thaumatin and Brazzein are genetically modified. Then, through codon optimization based on *Chlamydomonas reinhardtii* preference, the encoding genes for CrThaumatin and CrBrazzein are synthesized and cloned into the expression vector pGM6. The recombinant expression vector is introduced into wild-type *Chlamydomonas reinhardtii* sp. algal strains. Using the paromomycin resistance marker carried by the expression vector, the engineered algal strains are successfully obtained. The engineered algal strains are fermented to produce dried, stable *Chlamydomonas reinhardtii* powder rich in the target sweet protein. Finally, oral administration tests after protein purification show that its sweetness is 3-5 times that of natural Thaumatin and Brazzein proteins.

A sweetener according to exemplary embodiments comprises: a core including one selected from erythritol, xylitol, sorbitol, maltitol, mannitol, inositol, lactitol, isomalt, and allulose; and a shell surrounding at least a portion of the core and including one selected from stevia, enzyme-treated stevia, steviol glycoside, mogroside, thaumatin, sucralose, aspartame, saccharin, acesulfame potassium, and neotame, wherein a content ratio of the shell to the core may be 0.002 to 0.05. The sweetener has a core-shell structure, wherein the core and the shell are each made into a single raw material without any separate additives (e.g. excipients), and thus is close to zero calories.

The invention relates to the technical field of recombinant proteins, in particular to a pichia pastoris expression vector of thaumatin as well as a preparation method and application of the pichia pastoris expression vector. The expression vector comprises a strong promoter, a signal peptide and a target gene, the target gene is used for expressing thaumatin, and the nucleotide sequence of the target gene is shown as SEQ ID NO.1. The pichia pastoris expression vector realizes three key targets of high expression quantity, high purity and high activity of thaumatin through a core strategy of'strong start driven transcription + signal peptide guided efficient secretion 'on the basis of an optimized thaumatin target gene and characteristics of a strong promoter and a signal peptide of pichia pastoris; finally, a thaumatin product with the purity being 85% or above and the yield being 100 mg / L to 200 mg / L can be obtained.

The invention relates to the technical field of biology, in particular to a method for purifying sweet protein thaumatin II. The invention provides a method for purifying sweet protein thaumatine II from trichoderma reesei fermentation liquor, which can be used for effectively removing pigments, inorganic salts and impure proteins in the fermentation liquor so as to obtain high-purity thaumatine II protein. In specific implementation, solid-liquid separation is achieved through multiple times of high-speed centrifugation, after thalli in the trichoderma reesei fermentation liquor are removed, the pH of the fermentation liquor is adjusted to be 5-7, pigments in the fermentation liquor are removed through D201 resin, and then residual pigments are removed through activated carbon. And removing the activated carbon through high-speed centrifugation and three-stage filtration to obtain a crude extract. The preparation method comprises the following steps: firstly, extracting the thaumatine II protein, then, carrying out impure protein interception and concentration by using a 30kDa membrane bag and a 10kDa membrane bag, and finally, carrying out freeze drying on a concentrated solution, so as to obtain thaumatine II protein dry powder. According to the scheme provided by the invention, impurity pigments generated in thaumatin protein production can be quickly and effectively removed, the protein recovery rate and activity are not influenced, and the method has a wide application prospect.

The application discloses a sweet protein secretion expression system and a construction method and application thereof, belongs to the technical field of genetic engineering, and specifically discloses an engineered bacterium for expressing thaumatin, an expression vector, a molecular chaperone and an expression method. The thaumatin expressed by the engineered bacterium has a sweet taste after artificial trial, has higher purity and thermal stability compared with natural extraction thaumatin from African canes, the biosynthesis method is more moderate than a chemical extraction method, has higher yield, and is suitable for industrialization.

This invention discloses a mutant of the highly thermally stable sweet protein neoculin, its gene, and its applications. The neoculin comprises NAS and NBS chains. Mutations at positions 27, 51, 70, 93, and 95 of the NAS chain yield chain A; mutations at positions 11, 74, 78, 103, and 106 of the NBS chain yield chain B. A disulfide bond is formed between cysteine ​​residues at position 95 of chain A and 103 of chain B, resulting in a mutant of the highly thermally stable neoculin. Based on structural analysis and computationally assisted protein stability design, this invention employs novel disulfide bond design and site-directed mutagenesis to obtain a highly thermally stable neoculin mutant. Compared to the WT mutant, the T mutant of this invention exhibits significantly higher thermal stability. m It increased the temperature by at least 20.3°C while maintaining its sweetness.

The invention provides a thaumatin microcapsule and a preparation method thereof, a thaumatin standard sample in milk powder and a preparation method and a valuing method of the thaumatin standard sample, and relates to the technical field of analysis and detection. A core material of the thaumatin microcapsule is thaumatin, a wall material of the thaumatin microcapsule comprises sodium alginate, Arabic gum and carbomer, and the mass content of the thaumatin in the thaumatin microcapsule is 0.1-5%. According to the invention, thaumatin is encapsulated in the microcapsule and is added into the milk powder, so that the stability and uniformity of thaumatin in the milk powder can be improved, the accurate transmission of thaumatin amount in the milk powder is realized, and the effects of quality control, personnel examination and the like in a laboratory detection process are achieved. The thaumatin microcapsule provided by the invention is high in thaumatin content, the wall material is soluble in water, the thaumatin can be released from the microcapsule only through simple extraction, the constant value detection method of the thaumatin in the milk powder added with the thaumatin microcapsule is simple, and the detection result is accurate.

The present application relates to the technical field of genetic engineering and protein modification, and particularly relates to a thaumatin mutant and application thereof. The applicant screens a mutation site which significantly improves the sweetness of thaumatin by protein engineering technology, and overexpresses the mutation site in Pichia pastoris. The mutant can be used as a sweetener and widely applied in the field of feed or food processing.

A perfume composition is provided. The present invention relates to a composition comprising rutin and a sweetness modifier selected from the group consisting of mogroside, dihydrochalcone, hesperidin 3-acetate, brazilin, thaumatin, and combinations thereof.

Provided is an equol-containing composition in which bitterness is suppressed, and in particular, an equol-containing composition in which bitterness is suppressed and which is completely or equivalently unrestricted in food applications. The present invention provides an equol-containing composition comprising at least one masking agent selected from the group consisting of thaumatin and a siraitia grosvenorii extract, and an equol-containing substance, and if necessary, at least one sweetening agent selected from the group consisting of aspartame, neotame, Advantame, acesulfame, stevioside, curculiginine, molinin, monatin, synsepalum dulcificum protein, and sweet clostridium.

This invention discloses a liver-protective composition containing burdock root aqueous extract, its preparation method, and its application. The composition comprises six traditional Chinese medicinal herbs: burdock root aqueous extract, jujube seed extract, lily extract, wolfberry extract, white chrysanthemum extract, and poria cocos extract; seven nutrients: vitamin D3, vitamin E, inositol, choline, pyrrolizidine quinoline quinone, magnesium glycine, and zinc citrate; and three flavoring agents: semaside, mogroside, and adenosine monophosphate (AMP). AMP has both umami enhancement and AMPK activation effects. The composition is prepared into powder or tablets using low-temperature freeze-drying or low-temperature spray drying. The composition of this invention has a significant protective effect against chemically induced liver damage and sleep deprivation-induced liver damage, and is suitable for liver protection and health maintenance in people who stay up late, overuse their eyes, experience high mental stress, and are middle-aged and elderly.

To provide a tobacco composition that exhibits excellent sweetness persistence and suppresses off-flavors.SOLUTION: There is provided a tobacco composition comprising neotame, advantame, thaumatin and / or monellin. The tobacco composition is liquid and preferably contains neotame of 0.01 to 10 mass%, advantame of 0.01 to 5 mass%, and thaumatin and / or monellin of 0.01 to 15 mass%.SELECTED DRAWING: None

The invention provides a liquid chromatography-mass spectrometry detection method for thaumatin in food, and relates to the technical field of analysis and detection. According to the method, the trifluoroacetic acid aqueous solution is used as an extraction solvent to extract thaumatin in the to-be-detected food sample, and the thaumatin extraction rate is high. In the solid-phase extraction column purification and enrichment process, a PEP extraction column or a cation retin CX column is adopted, the thaumatin retention effect is good, 60-75 v / v% methanol-ammonia water serves as an eluent, the thaumatin recovery rate is high, the enrichment effect is good, and the impurity removal effect is good. Trypsin is used for carrying out enzymolysis on the purified liquid obtained through nitrogen blowing ammonia removal and methanol constant volume, obtained enzymatic hydrolysate is subjected to solid-phase extraction column desalination, and a characteristic peptide fragment of thaumatin can be obtained; by adopting liquid chromatography-mass spectrometry detection, accurate qualitative and quantitative detection of I and thaumatin II in food can be realized.

The invention provides a sweetener or flavor modifying composition comprising Thaumatin and Brazzein.

The invention relates to the technical field of biology, in particular to a method for purifying sweet protein thaumatin I. The method comprises the following steps of: preparing thaumatin I; the invention provides a method for purifying sweet protein thaumatine I from Trichoderma reesei fermentation liquor, which can effectively remove pigments, inorganic salts and impure proteins in the fermentation liquor so as to obtain high-purity thaumatine I protein. Thalli and fermentation liquor are subjected to solid-liquid separation through high-speed centrifugation, the pH value is adjusted to 5-7, and pigment and impure protein are removed through D201 resin and activated carbon. After the activated carbon is removed through high-speed centrifugation and three-stage filtration, impure protein is intercepted by using 30kDa and 10kDa membrane coating technologies, and target protein is concentrated. And finally, carrying out freeze drying to obtain Thaumatin I protein dry powder. The method can efficiently remove impurities and maintain protein activity, is simple and convenient to operate, and has important application value.

The present invention relates to an orally disintegrating tablet comprising pregabalin or a pharmaceutically acceptable salt thereof, sucralose as a sweetener, and tomatine.

The present invention provides orally administered products and compositions that have reduced sugar content and achieve the same or very similar taste quality as conventional products in terms of sweetness, overall taste, and aftertaste. [Solution] A product for oral intake, such as food, comprising at least one thaumatin selected from the group consisting of thaumatin I and thaumatin II, and at least one sugar selected from the group consisting of sucrose, glucose, and fructose, in a mass ratio of thaumatin to sugar of 1:2,000 to 1:80,000.

The present disclosure relates to the technical field of biological fermentation, and in particular, to a recombinant yeast cell and its use in producing thaumatin. The recombinant yeast cell comprises nucleic acids corresponding to the genes of thaumatin, PDI and MSN4, and can functionally express the same. The present disclosure provides a recombinant yeast cell co-expressing chaperone PDI and transcription factor MSN4 to improve the secretion expression of thaumatin protein. Surprisingly, under the synergistic effect of chaperone PDI and transcription factor MSN4, the secretion expression of exogenous thaumatin protein is significantly increased by 3.25 times.

Provided is a use for a sweet-tasting protein in reducing body weight, the sweet-tasting protein being brazzein or thaumatin. The provided sweet-tasting protein significantly inhibits high-fat diet-induced weight gain in subjects, and alleviates to a certain extent symptoms of insulin resistance caused by obesity, allowing for resumption of normal insulin secretion in subjects, lowering blood lipids, and ameliorating liver damage caused by high-fat diets, indicating that the sweet-tasting protein is effective in reducing body fat and decreasing body weight.

The present invention discloses an amylase signal peptide variant mutant SEQ ID NO: 2 or 3 generated by a natural language model. The encoding gene thereof is fused with a bovine lactoferrin gene or a thaumatin gene and expressed in Aspergillus. The variant mutant can improve the secretion and expression of bovine lactoferrin and / or thaumatin by Aspergillus and enhance the production economy of bovine lactoferrin or thaumatin.

The invention belongs to the technical field of RNA biological prevention and treatment, and particularly relates to application of dsRNA of a targeted antigenic sweet-like protein coding gene. The invention provides application of dsRNA of a targeted antigenic sweet-like protein coding gene in prevention and treatment of crop anthracnose, the dsRNA is obtained by transcription of a part of sequence of the antigenic sweet-like protein coding gene CsATLP1, and by loading the dsRNA on a nano-material layered double hydroxide, pathogenicity of colletotrichum rubrum and colletotrichum mangiosporioides can be obviously reduced, and the resistance of the colletotrichum rubrum and colletotrichum mangiosporioides can be improved. The application prospect of efficient and targeted prevention and treatment of crop anthracnose is realized.

The present application relates to a kind of for detecting helicobacter pylori and its preparation method, by weight parts, its raw material includes: urea [ 13 C]10~75 portions, sodium bicarbonate 5~110 portions, lactose 15~45 portions, citric acid 1000~2500 portions, flavoring agent 50~150 portions.The flavoring agent includes at least one of stevioside, sucralose, aspartame, neotame, thaumatin, acesulfame potassium.The present application adopts the way of citric acid and tablet storage separately, effectively solves the problem of flavoring agent addition, avoids the problem of impurity generation due to citric acid addition;At the same time, mannitol is not used in medicine, reduces the risk of patient using medicine.