Thaumatin-like protein related to wheat leaf rust resistance and encoding gene and application thereof

Abstract

The invention discloses a thaumatin-like protein gene TcLr19PR5 related to wheat leaf rust resistance and a gene encoded through the thaumatin-like protein gene TcLr19PR5. Through RT-PCR and the electronic cloning technology, a 516bp thaumatin-like protein gene is cloned, and 171 amino acid residues are coded; and real-time fluorescence quantification PCR analysis shows that at different time points after puccinia triticina is inoculated, the expression quantity of the gene in non-affine combinations is higher than that of the gene in affine combinations, it is indicated that expressions are induced through the wheat puccinia triticina, the expression amount of stems is higher than the expressions of the leaves and the roots, and meanwhile it is clear that the expressions are induced by adversity signal molecules of abscisic acid (ABA) and salicylic acid (SA). According to the thaumatin-like protein gene and the gene, basic data are provided for in-depth exploration of wheat and puccinia triticina interaction molecular mechanisms, and the theory basis is provided for cultivating leaf-rust-resistance wheat varieties.

Description

technical field [0001] The invention relates to a sweet-like protein related to wheat leaf rust resistance, its encoding gene and application. Background technique [0002] wheat( Triticum aestivum Linn.), as an important food crop, occupies an important position in the food industry and agricultural production (Cao Yaping, 2008). Wheat leaf rust is one of the most important diseases affecting wheat yield and quality (Bolton et al., 2008). In recent years, plant genetic engineering technology has developed rapidly, and the molecular breeding approach centered on the cloning and transformation of disease resistance genes and various resistance-related genes has become a new direction of breeding. [0003] Disease process-related proteins refer to a class of proteins encoded by host plants and induced in pathological or pathologically related environments (Van et al., 1999). According to the classification system based on plant origin, electrophoretic mobility (molecular w...

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Problems solved by technology

[0004]Because the wheat genome is relatively large (1.6×109 bp) and complex in structure, the number of defense genes directly cloned from it is small, and these Factors greatly limit the research on the mechanism of wheat leaf rust resistance and the storage and utilization of resistance resources

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