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Screening method for multigene pedigree of Cylindricaceae

A technology of columnar rusts and genes, applied in the field of multi-gene lineage screening of columnar rusts, can solve problems such as economic losses, threats, and ecological damage

Active Publication Date: 2022-05-10
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These fungi are a serious threat to artificial or natural pine forests, and once they become a disaster, they will cause huge economic losses and ecological damage

Method used

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  • Screening method for multigene pedigree of Cylindricaceae
  • Screening method for multigene pedigree of Cylindricaceae
  • Screening method for multigene pedigree of Cylindricaceae

Examples

Experimental program
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Effect test

Embodiment 1

[0050] Embodiment 1, the construction and use method of multi-gene selection of Cylindromycetes and its typing screening database

[0051] 1. Multi-gene selection and typing screening database construction of Cylindromycetes

[0052] 1. Multi-gene selection of Cylindromycetes

[0053] Collect the following two gene sequences of the existing Cylindromycetes species: ITS and LSU;

[0054] The above-mentioned two gene sequences of the existing Pyropyridaceae species can be derived from the ITS and LSU gene sequences of all Pyropyridaceae species patterns and voucher patterns in the NCBI database; they can also be from the missing existing gene sequences in the collected NCBI database. ITS and LSU gene sequences in Cylindropyraceae species.

[0055] In the embodiment of the present invention, the ITS and LSU gene sequences of the existing Pyrocorticaceae species used are shown in Table 1 below.

[0056] 2. Build a database

[0057]Table 1 below shows all the ITS and LSU gene s...

Embodiment 2

[0089] Example 2, the application of the multigene reference sequence screening database of the Pillarphyllaceae species certificate specimens and bacterial strains in identifying whether the pathogen to be tested is Pyrophyllaceae

[0090] 1. Obtain the gene sequence of the bacteria ITS and LSU to be tested

[0091] Cronartium coleosporioides specimens MICH253346, C. comandrae specimens MICH253364, C. ribicola specimens NY267053 and Pine peony Specimen HMAS82784 of rust fungus (C.flaccidum) and specimen HMAS247968 of Larix poplar rust fungus Melampsora larici-populina in the family Psoriasiaceae were used as the pathogens to be tested.

[0092] The genomic DNA of 6 specimens to be tested in Cylindromycetaceae and 1 specimen in Pascariaceae were extracted respectively, and the primer pairs of these 3 genes (Table 2) were used to amplify these 2 genes respectively, and the amplified products were sequenced respectively , to obtain the three gene sequences of ITS and LSU of the...

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Abstract

The invention discloses the preparation and application of a multigene pedigree screening database of the Cylindricaceae. The present invention provides the application of the substances shown in a) and b) in identifying or distinguishing the species of Cylindricaceae, and the substance shown in a) is a substance for detecting the following gene sequence in the sample to be tested or the pathogenic bacteria to be tested: ITS and LSU; the material shown in b) is the ITS and LSU gene sequences in the existing Cylindropyraceae species and various genus; the present invention uses the ITS and LSU gene sequences to carry out the construction of the evolutionary tree and the homology comparison, which can Detecting Pillarphyllaceae, at the quarantine port, can quickly and accurately screen samples containing Pillarphyllaceae fungi to prevent the spread of germs along with the import, export and transportation of seeds and other reproductive materials, so as to protect the healthy development of agricultural production in our country , is of great significance.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a screening method for a multigene genealogy of Cylindricaceae. Background technique [0002] The causative agent of pine blister rust, which causes one of the three major fungal diseases harmful to forest trees in the world, consists of species of the phytopathogenic fungus Cylindricaceae. The species of this family belong to the family Basidimycota, class Pucciniomycetes, and the family Cronartiaceae of the order Pucciniales. There are only two genera in this family, Cronartium and Endocronartium. Among them, Pillar rust fungi belonged to Pillar rust fungi in 2019. At present, the entire family contains 1 genus and a total of 36 species. These fungi pose a serious threat to artificial or natural pine forests, and once they become a disaster, they will cause huge economic losses and ecological damage. [0003] Therefore, there is a need to find a method for the identification of Cr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12Q1/04
CPCC12Q1/6895
Inventor 蔡磊赵鹏刘芳
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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