42 results about "Puccinia graminis tritici" patented technology

The invention discloses a molecule detection method for puccinia striiformis f.sp.tritici No.17 physiological race. By employing a method for converting a RAPD molecular marker into a SCAR molecular marker, the specific primers (upstream primer 5' -TTCCCCCGCTGTCT and downstream primer 5'- GTTGAGCGGGGGAA) of No.17 physiological race are obtained, and are subjected to PCR amplified reaction (reaction conditions: 94 DEG C, 5 min, 1 cycle; 94 DEG C, 30 s, 60 DEG C, 1 min, 72 DEG C, 90 s, 35 cycles; 72 DEG C, 7 min, 1 cycle) and 2% agarose gel electrophoresis, so that an specific strip with a length of about 1000 bp is obtained. Therefore, the molecule detection method helps to rapidly discriminate and distinguish puccinia striiformis f.sp.tritici No.17 physiological race in molecular level, and establishes a base for developing research on wheat stripe rust molecular epidemiology.

The invention discloses a thaumatin-like protein gene TcLr19PR5 related to wheat leaf rust resistance and a gene encoded through the thaumatin-like protein gene TcLr19PR5. Through RT-PCR and the electronic cloning technology, a 516bp thaumatin-like protein gene is cloned, and 171 amino acid residues are coded; and real-time fluorescence quantification PCR analysis shows that at different time points after puccinia triticina is inoculated, the expression quantity of the gene in non-affine combinations is higher than that of the gene in affine combinations, it is indicated that expressions are induced through the wheat puccinia triticina, the expression amount of stems is higher than the expressions of the leaves and the roots, and meanwhile it is clear that the expressions are induced by adversity signal molecules of abscisic acid (ABA) and salicylic acid (SA). According to the thaumatin-like protein gene and the gene, basic data are provided for in-depth exploration of wheat and puccinia triticina interaction molecular mechanisms, and the theory basis is provided for cultivating leaf-rust-resistance wheat varieties.

The invention relates to specific primers and a molecule detection method for pathogenic fungi of austral puccinia sorghi. The specific primers comprise a forward primer 5'-CTCCAAGAACTTCCTCCTC-3' and a reverse primer 5'-TGACATGAAGTAGAAATTCT-3'. The primers are subjected to PCR (Polymerase Chain Reaction) amplification and agarose gel electrophoresis, and the bacteria bearing condition of corns infield can be detected by adopting the primers and amplification products with segment length of 540bp, obtained through PCR amplification, wherein PCR amplification comprises the following steps of: pre-modifying for 5 minutes at 95 DEG C; then modifying for 45 seconds at 94 DEG C, annealing for 45 seconds at 55 DEG C, stretching for 90 seconds at 72 DEG C, and repeating the steps for 30 cycles; and finally stretching for 10 minutes at 72 DEG C. The primers disclosed by the invention have strong specificity for corn puccinia strilformis, can be used for detecting corn rust diseases and has high sensitivity up to 0.001 ng/mu m L. The detecting method disclosed by the invention has good practicability and is simple, convenient and quick to operate.

The invention belongs to the technical field of disease-resistant variety cultivation, and discloses an acquisition of edited wheat susceptible gene plants and application of edited wheat susceptiblegene plants in disease-resistant variety cultivation. The edited wheat susceptible gene plants have B, D and A copies in wheat. The RNAi mutants of the TaRIPK1 with two genetic backgrounds are obtained. According to the invention, an agrobacterium tumefaciens-mediated genetic transformation method is adopted, and a CRISPR-Cas9 gene editing technology is utilized to obtain the gene edited plant ofTaRIPK1. Through inoculation of wheat stripe rust bacteria, it is found that TaRIPK1 RNAi plant bacteria with two genetic backgrounds have remarkable resistance to stripe rust bacteria toxic race CYR32, disease resistance identification is conducted on a TaRIPK1 gene editing technology, and it is found through results that the disease resistance of TaRIPK1 gene editing plants is remarkably improved. The resistance to main epidemic races of puccinia striiformis and puccinia leaf rust is remarkable, and the plants can be used as a germplasm new material for genetic improvement of wheat rust resistance.

The invention discloses a molecular detection method for rapid detection of new toxic strain V26 of puccnia striiformis West f.sp.tritici; by a large number of screening, a specific marker of the new toxic strain V26 of the puccnia striiformis West f.sp.tritici is obtained, the specific marker is sequenced, and according to sequencing results, a pair of specific primers comprising V26SP1:5'CTGTAAAGCGGATAAAGGAA 3', and V26SP2:5'CATAAGAGCCACACTTGACC3'. Optimal PCR (polymerase chain reaction) conditions of the pair of the specific primers are established. Different single spore strains of the V26 strain is detected by use of an optimal PCR method, and specific SCAR (sequence characterized amplified regions) markers of the V26 can be obtained by respectively taking of other 13 physiological races and ultra pure water as controls. The molecular detection method is mainly used for early-stage rapid detection of the V26 strain in a large field, the dynamic variation conditions can be known, and the molecular detection method provides reliable technical support and theoretical basis for monitoring of the strain and prevention and control decisions of wheat yellow stripe rust disease.

The invention discloses a sterilizing composition. The effective components are Sedaxane and captan, the mass ratio of the both is 50:1-1:50. The composition has obvious synergism and is effective totreat diseases caused by rhizoctonia solani, puccinia horiana, pyrenophora graminea, ustilago esculenta, Tilletia, Erysiphe, pyrenophora teres, Septoria musiva, and Ramularia; the composition has special effect to seed-borne disease. The composition can be applied to treat seeds and spray stems and leaves, the effect duration lasts for 4-6 weeks; the composition can be applied to the disease prevention and treatment of cereal type crops; particularly, it is applied to the seed treatment for preventing wheat ustilago esculenta, wheat tilletia, wheat basal rot, sphacelotheca reiliana, corn stalkrot, corn gall smut and rice bakanae disease.

The present invention relates to farm chemical with glucoside alkaloid as the effective component for preventing and controlling plant mycosis and its application. The fungicidal glucoside alkaloid preparation has up to five kinds of glucoside alkaloid with obvious effect of preventing and controlling plant diseases caused by Verticillium, monospermous rust fungi, Fusarium and other fungi. The fungicidal glucoside alkaloid preparation has broad spectrum, low toxicity, environment friendship, low cost and other features.

The invention discloses a method for forecasting a kendir rust disease caused by kendir Melampsora and a special device therefor. The method mainly comprises the following steps: manufacturing of a carrier, preparation of a sampling device, sampling and trapping of spores, isolated culture and pathogenicity calculation. The occurrence of the Melampsora of the field-planted kendir can be accurately forecast and predicted, so that a control plan can be effectively made. Compared with the prior art, the invention can effectively determine the quantity of pathogenic bacteria having infectivity while the quantity of pathogenic bacteria can be determined, and especially can solve the problem that isolated kendir leaves which have been sampled in an aeroscope can be easily polluted and rotten in the culture process. Thus, the invention is suitable for forecasting and predicting the Melampsora of the field commercially-planted kendir.

The invention discloses a puccinia graminis chitin deacetylase as well as a preparation method and application thereof. According to codon preference of pichia pastoris, an optimized nucleic acid sequence is shown as SEQ ID NO.2, and a chitin deacetylase encoding gene sequence of the puccinia graminis is obtained by utilizing a whole-gene synthesis method. Furthermore, a pichia pastoris expressionsystem is used for outperforming secretory expression on the optimized chitin deacetylase encoding gene to obtain chitin deacetylase, and the amino acid sequence of the chitin deacetylase is shown asSEQ ID NO.1. The obtained chitin deacetylase disclosed by the invention can be used for removing acetyl in chitin, chitosan and chitosan oligosaccharide, so that the chitosan or the chitosan oligosaccharide adopting a specific structure is obtained. The chitosan or the chitosan oligosaccharides adopting specific structures can have new or higher biological activity. Therefore, the puccinia graminis chitin deacetylase and a deacetylation product thereof have good industrial application prospects.

The invention discloses 'a monoclonal antibody, an immune fluorescence method and a kit for detecting puccinia striiformis f.sp.tritici, puccinia triticina f.sp.tritici and puccicinia graminis f.sp.tritici' and belongs to the field of agricultural biotechnology. The invention mainly provides the monoclonal antibody for detecting puccinia striiformis f.sp.tritici, puccinia triticina f.sp.tritici and puccicinia graminis f.sp.tritici, which is secreted by hybridoma cell line with a collection number CGMCC No.4414 and can identify puccinia triticina f.sp.tritici, puccicinia graminis f.sp.tritici and puccinia striiformis f.sp.tritici at the same time. The invention also provides a detection method based on the combination of the monoclonal antibody and an immune fluorescence technology and a kit. The monoclonal antibody, the detection method and the kit have the advantages of high specificity, high sensitivity, and simplicity and quickness of operation.

The invention belongs to the field of gene engineering, and discloses a small GTP binding protein gene TaRab18 and an expression vector and application thereof. The cDNA sequence of the small GTP binding protein gene TaRab18 is SEQ ID NO.1, and the sequence amino acid coded by the small GTP binding protein gene TaRab18 is SEQ IDNO.2. The gene comes from ordinary wheat (Triticum asetivum L.)92R137. The puccinia striiformis induction expression of TaRab18 in the stripe rust prevention wheat variety 92R137 is enhanced, the expression level of TaRab18 is far higher than the expression level in susceptible wheat variety Yangmai 158. An over-expression vector is obtained by inserting the gene in pBI220, susceptible wheat variety Yangmai 158 is converted, the result of substituting T1 of positive transformation plants to the stripe rust identification shows that the resistance of the susceptible wheat variety to the stripe rust can be improved through the over-expression of TaRab18.

The invention belongs to the technical field of biological prevention and control, and discloses a biological method for inhibiting rapid growth of mikania micrantha, which comprises the following steps: measuring the safety of mikania micrantha puccinia peucedanum on non-target organisms; determining the mikania micrantha puccinia infection process; determining the phenotype of mikania micrantha growth inhibition by mikania micrantha puccinia Determining the inhibition of mikania micrantha hormone synthesis by mikania micrantha puccinia And determining the inhibition of mikania micrantha puccinia peucedanum on the photosynthesis of Through measurement of the net photosynthetic rate and the stomatal conductance of mikania micrantha leaves and content analysis of Kalvin cycle pathway related metabolites, it is found that after mikania micrantha puccinia is infected, the photosynthetic rate and the stomatal conductance of the leaves can be remarkably reduced, the content of Kalvin cycle pathway related compounds is inhibited, the net photosynthetic rate is reduced by 10.57%, and the content of Kalvin cycle pathway related metabolites is reduced by 10.57%. The stomatal conductance is reduced by 26.03%, and the content of Kalvin cycle pathway related compounds is reduced by 1.9-3.4 times.

The present invention relates to a method of increasing resistance against fungal pathogens of the order Pucciniales in plants and/or plant cells. This is achieved by increasing the expression of a MybTF protein or fragment thereof in a plant, plant part and/or plant cell in comparison to wild type plants, wild type plant parts and/or wild type plant cells. Furthermore, the invention relates to transgenic plants, plant parts, and/or plant cells having an increased resistance against fungal pathogens, in particular, pathogens of the order Pucciniales, and to recombinant expression vectors comprising a sequence that is identical or homologous to a sequence encoding a MybTF protein.

The invention relates to a real-time fluorescent quantitative PCR technology-based detection method and a kit for corn southern rust pathogenic bacteria puccinia polypoda. Specific primers and TaqMan probes are designed according to ITS of corn southern rust and Actin2 gene sequences of corn, and a PCR quantitative detection system for the incubation period of the corn southern rust is established on the basis of the two pairs of primers and probes. The primers and the probes for the corn southern rust fungus and the corn have the characteristics of high specificity and sensitivity. The PCR quantitative detection system for the puccinia multiflora incubation period, established by the invention, not only can detect the puccinia multiflora in the incubation period, but also can detect the infection amount at different time after infection, and the system can be used for early detection and quantitative detection of the puccinia multiflora. The research method provided by the invention can be effectively applied to early molecular detection of puccinia polypoda, and has important significance in the aspects of early prediction, forecast, comprehensive treatment and the like of the corn southern rust disease.

The invention belongs to the technical field of development and research of feed additives, and specifically discloses an animal growth promoter containing Puccinia reedi, which contains Puccinia reedi with the nucleotide sequence shown in SEQ ID NO.1. Preparation method: 1. collection of teliospores of Puccinia reed; 2. drying; 3. crushing of reed leaves; 4. crushing of teliospores of Puccinia reed; In the invention, the teliospores of Puccinia reedi are broken and fed to animals in the form of feed additives, which can significantly promote the growth of animals in practice.

The present invention relates to a method of increasing resistance against fungal pathogens of the order Pucciniales, preferably the family Phacopsoraceae, in plants and/or plant cells. This is achieved by increasing the expression of an HCP4 protein or fragment thereof in a plant, plant part and/or plant cell in comparison to wild type plants, wild type plant parts and/or wild type plant cells. Furthermore, the invention relates to transgenic plants, plant parts, and/or plant cells having an increased resistance against fungal pathogens, in particular, pathogens of the order Pucciniales, preferably the family Phacopsoraceae, and to recombinant expression vectors comprising a sequence that is identical or homologous to a sequence encoding an HCP4 protein.