33 results about "Wheat leaf rust" patented technology

The invention provides a barley transcription factor HvbZIP10 gene and its application in wheat resistance to stripe rust and leaf rust. The invention relates to gene sequences, and specifically discloses barley transcription factor HvbZIP10, the nucleotide sequence of which is shown in SEQ ID No.1, and the preparation process of wheat transgenic materials overexpressing HvbZIP10. The invention verifies through experiments that the HvbZIP10 gene can significantly improve the resistance level of wheat to wheat stripe rust and wheat leaf rust.

The present invention provides a molecular marker and its application for rapid detection of the leaf rust resistance gene of E. elongatum. With the aid of the induced short-segment translocation line of E. elongatum for resistance to leaf rust, combined with the reference sequence of the wheat D genome, The molecular marker Xsdau798 closely linked to it was designed and developed for wheat leaf rust resistance breeding and early molecular marker-assisted selection of leaf rust resistance traits to improve breeding efficiency.

The invention relates to an antigen and a polyclonal antibody for identifying wheat puccinia recondita and wheat puccinia graminis. An antigen polypeptide for preparation of the polyclonal antibody for distinguishing wheat puccinia recondita and wheat puccinia graminis has an amino acid sequence shown in SEQ ID NO:1. The immunogen for preparation of the polyclonal antibody for distinguishing wheat puccinia recondita and wheat puccinia graminis is characterized in that the immunogen is prepared from the antigen polypeptide as a hapten. The polyclonal antibody for distinguishing wheat puccinia recondita and wheat puccinia graminis is characterized in that domestic rabbits are immunized by the immunogen, and a high-titer immunized domestic rabbit is detected and screened; and whole blood of the selected domestic rabbit is taken, and the polyclonal antibody is obtained through purification. The method for distinguishing wheat puccinia recondita and wheat puccinia graminis can simply and effectively detect whether wheat suffers from early infection of puccinia recondita.

The invention provides a wheat disease process-related protein TaPR1a gene and its application in wheat resistance to stripe rust and leaf rust. The invention relates to a gene sequence, and specifically discloses a wheat disease process-related protein TaPR1a gene, the nucleotide sequence of which is shown in SEQ ID No.1, and a preparation process of a wheat transgenic material overexpressing the TaPR1a gene. The invention verifies through experiments that the TaPR1a gene can significantly improve the resistance level of wheat to wheat stripe rust and wheat leaf rust.

The invention provides a colloidal gold test strip used for detecting wheat leaf rust. The colloidal gold test strip comprises a sample pad, a colloidal-gold pad, an NC membrane, absorbent paper and a PS base plate, wherein the sample pad, the colloidal-gold pad, the NC membrane and the absorbent paper are successively arranged on the PS base plate along the chromatographic direction of a sample, the colloidal-gold pad is coated by a colloidal gold-labeled monoclonal antibody against Puccinia triticina, the NC membrane successively comprises a detection line and a quality control line along the chromatographic direction of the sample, the detection line is coated with Puccinia triticina antigen protein that the monoclonal antibody can specifically recognize, and the quality control line is coated with a goat anti-mouse secondary antibody. The monoclonal antibody against Puccinia triticina is secreted by a hybridoma cell with an accession number of CGMCC No. 10884. When the test strip is used for detection, the process from sampling to obtainment of detection results only takes 15 min, operation is simple and convenient, the results are accurate, early warning of wheat leaf rust can be realized, and economic loss caused by diseases is reduced.

The invention discloses a SNP molecular marker TC113325 of wheat leaf rust resistance gene Lr42, a method for detecting wheat leaf rust resistance gene Lr42 using the molecular marker and its application. The molecular marker is amplified by the following primers, wherein the sequence of the upstream primer is as follows: As shown in SEQ ID NO.1 and SEQ ID NO.2, the downstream primer sequence is shown in SEQ ID NO.3, using the above SNP molecular marker TC113325 to detect wheat leaf rust resistance gene Lr42 has high detection throughput, short time and accuracy High, easy to realize automation, low cost, environment-friendly and other characteristics, it is conducive to large-scale application in breeding work.

The invention discloses a primer used for screening wheat brown leaf rust resisting QTL and application thereof and relates to the technical field of nucleic acid determination or test methods. The QTL is located on a chromosome 2DS, the marker interval is Xgwm261-Xgwm484, and the QTL is from a susceptible parent Thatcher; the primer is a primer pair composed of upstream and downstream nucleotide sequences of a primer wms261 and a primer wms484. The primer is used for screening wheat brown leaf rust resisting QTL-QLr.hebau-2DS, and molecular markers in close linkage with the QTL can be used for marking the QTL for auxiliary selection, so that accumulation of multiple effective anti-disease genes are achieved, and disease resistance of varieties is improved. The discovered novel brown leaf rust resisting QTL and the special primer play an important role in wheat disease resisting breeding process.

Relating to the field of plant protection, the invention specifically discloses a primer set of a puccinia recondita tritici EST-SSR molecular marker, a detection method and application thereof. The primer set of the Puccinia recondita tritici EST-SSR molecular marker includes primers shown as nucleotide sequences SEQ ID No:1-37. The detection method for puccinia recondita tritici genetic diversity employs the primer set to analyze the puccinia recondita tritici diversity and the population genetic structure, has the characteristics of simple operation, convenient detection, stable PCR amplification result, clear electrophoretic band and rich polymorphism, plays an important role in puccinia recondita tritici toxicity monitoring and genetic diversity analysis.

The invention provides wheat leaf rust resistance-related KASP molecular markers and application thereof. The KASP molecular markers are selected from BA00138075 and / or BA00606902, wherein base sequences of BA00138075 and BA00606902 are as shown in a table 1 as shown in the specification respectively. According to the provided KASP molecular markers, the selectivity of a locus can be enhanced, theaccuracy of selection of a leaf rust-resistant breeding material is improved, and the provided KASP molecular markers have important significance for achieving molecular marker-assisted selection breeding and improving the selection efficiency of durum wheat breeding.