33 results about "Wheat leaf rust" patented technology

A novel strain of Bacillus subtilis EB120 shows high antagonistic activity for controlling various plant diseases including barley powdery mildew, cucumber powdery mildew, red pepper anthracnose, rice blast, tomato gray mold, tomato late blight and wheat leaf rust, it can be effectively used as a microbioside for biologically controlling the plant diseases.

The invention discloses a thaumatin-like protein gene TcLr19PR5 related to wheat leaf rust resistance and a gene encoded through the thaumatin-like protein gene TcLr19PR5. Through RT-PCR and the electronic cloning technology, a 516bp thaumatin-like protein gene is cloned, and 171 amino acid residues are coded; and real-time fluorescence quantification PCR analysis shows that at different time points after puccinia triticina is inoculated, the expression quantity of the gene in non-affine combinations is higher than that of the gene in affine combinations, it is indicated that expressions are induced through the wheat puccinia triticina, the expression amount of stems is higher than the expressions of the leaves and the roots, and meanwhile it is clear that the expressions are induced by adversity signal molecules of abscisic acid (ABA) and salicylic acid (SA). According to the thaumatin-like protein gene and the gene, basic data are provided for in-depth exploration of wheat and puccinia triticina interaction molecular mechanisms, and the theory basis is provided for cultivating leaf-rust-resistance wheat varieties.

The invention relates to a method for identifying a wheat leaf rust single sorus by utilizing nested PCR (polymerase chain reaction). The method comprises the following steps: designing and synthesizing a primer, extracting DNA (deoxyribonucleic acid) of the single sorus, performing amplification and separation on target genes, performing separation and identification on an amplified final product, then performing sequencing, comparing in a Genbank and determining the wheat leaf rust single sorus. The method for identifying the wheat leaf rust single sorus by utilizing the nested PCR is simple to operate and rapid and accurate in identification result, and can be applied into actual production.

The invention relates to water dispersible granules containing pyraclostrobin. The water dispersible granules containing pyraclostrobin comprise the following ingredients: 5%-80% of active ingredients, 1%-10% of a dispersing agent, 1%-8% of a wetting agent, 1%-10% of a disintegrating agent, 1%-5% of a binding agent and the balance of a filler. The preparation method comprises the steps of performing uniform mixing, ultrafine grinding, granulation, drying and sieving on the raw materials to obtain the water dispersible granules. The two compounded granules have an obvious synergistic effect than a mono-agent, the problems of difficult disintegration and the like of single pyraclostrobin or compounded water dispersible granules due to low melting point is solved, and the defects of dust flying and the like of wettable powder during production and use processes are overcome; the water dispersible granules have the advantages of high active ingredient content, high disintegration speed, good dispersity, high thermal stability, less environmental pollution and the like, has good control effects on wheat leaf rust, powdery mildew of cucumber, alternania mail Roberts and macrophoma musae when in use, and is high in safety.

The invention relates to an SNP (single nucleotide polymorphism) site of an anti-wheat leaf rust gene Lr13 and application of the SNP site. The SNP site is positioned at a 157695819 locus of a 2B chromosome of wheat, and a KASP (kompetitive allele specific polymerase chain reaction) primer combination capable of amplifying the SNP site comprise three primers of SEQ ID NO.2, SEQ ID NO.3 and SEQ IDNO.4. The SNP site interlinked with the anti-wheat leaf rust gene Lr13 is screened, designed and developed, in addition, the KASP primer combination is designed for the SNP site, the type of the SNP site can be accurately identified through the KASP primer combination, then a wheat strain with the anti-wheat leaf rust gene Lr13 can be screened, the selection efficiency can be improved, the anti-disease breeding progress of wheat can be accelerated, and the target of pyramiding breeding of multiple anti-disease genes can be achieved.

The invention provides wheat leaf rust resistance-related KASP molecular markers and application thereof. The KASP molecular markers are selected from BA00138075 and/or BA00606902, wherein base sequences of BA00138075 and BA00606902 are as shown in a table 1 as shown in the specification respectively. According to the provided KASP molecular markers, the selectivity of a locus can be enhanced, theaccuracy of selection of a leaf rust-resistant breeding material is improved, and the provided KASP molecular markers have important significance for achieving molecular marker-assisted selection breeding and improving the selection efficiency of durum wheat breeding.

The invention discloses a method for grading wheat leaf rust based on infrared thermal imaging edge detection processing, which relates to the technical field of wheat rust detection. Firstly, the infrared thermogram of the whole wheat sample was collected to calculate the average leaf temperature of healthy plants, incubated plants and symptomatic plants, respectively, and to find out the temperature change rule in the process of fungal invasion. Then the regions of infrared thermogram which were pretreated by histogram equalization and median filter were extracted from the infrared thermogram. The proportion of lesion area in the total thermal imaging area of the whole plant was calculated by dividing the temperature region, extracting the low temperature region and segmenting the threshold value. The correlation coefficient was 0.9755, RMSE was 9.79%, and the overall recognition rate was 90%. Compared with the general near-infrared detection method, the invention has the advantagesof fast detection speed and earlier detection of diseases.

The invention discloses a primer for screening wheat leaf rust resistance QTL and an application of the primer, and relates to the technical field of determination or detection method of nucleic acid. The primer is a primer pair consisting of upstream and downstream nucleotide sequences of primers wmc692 and wmca657. The invention provides novel SSR markers wmc692 and wmc657 of the wheat leaf rust resistance QTL-(i) QLr.zh-4B. A molecular marker which is tightly linked to the QTL can be utilized to perform the assistant marker selection for the QTL, so that the accumulation of a plurality of active disease-resistant genes can be realized, and the disease resistance of a species can be prolonged. The novel leaf rust resistance QTL and the special primer can play an important role in the long-term disease resistance breeding of wheat.

The invention relates to an indel molecular marker of a wheat leaf rust resistance gene Lr13 and an application of the indel molecular marker. The indel molecular marker is located at the 1577558888thsite of a wheat 2B chromosome, and the nucleotide sequence of the indel molecular marker is shown in the formula of SEQ ID NO.1. According to the invention, genetic analysis is carried out on disease-resistant and susceptible wheat, the interval position of the leaf rust resistance gene Lr13 is determined by constructing a genetic linkage map, an indel molecular marker linked with the gene is correspondingly developed, the indel molecular marker is highly linked with the wheat leaf rust resistance gene Lr13, a wheat strain containing the wheat leaf rust resistance gene Lr13 can be screened out through the indel molecular marker, the selection efficiency is improved, the wheat disease-resistant breeding process is accelerated, and the purpose of polymerization breeding of multiple disease-resistant genes is achieved.

The invention provides a wheat disease course related protein TaPR1a gene and an application thereof in wheat stripe rust and leaf rust resistance. The invention relates to a genetic sequence, and particularly discloses the wheat pathogenesis related protein TaPR1a gene with a nucleotide sequence as shown in SEQ ID No.1, and a preparation process of a wheat transgenic material for overexpressing the TaPR1a gene. Experiments prove that the TaPR1a gene can significantly improve the resistance level of wheat to wheat stripe rust and wheat leaf rust.

The invention provides a method for improving dcaps marker detection efficiency, and relates to the technical field of molecular biology. The method for improving the dcaps marker detection efficiencycomprises the following steps: S1, selecting a target gene sequence: extracting a known wheat leaf rust resistance gene Lr67 and a wild type susceptibility gene thereof; S2, designing a dCAPS primer:extracting the wheat leaf rust resistance gene Lr67 in S1 to be matched with dCAPS Finder2.0 equipment to prepare a primer with a normal sequence length; and S3, amplifying the primer sequence length: amplifying the primer prepared in S2. According to the method for improving the dcaps marker detection efficiency, enzyme cutting sites are introduced into primers of dCAPS, and the length of the primers is generally about 20 bp, so that fragments which are about 20 bp shorter than original fragments are generated after PCR enzyme cutting, and polyacrylamide gel electrophoresis is generally adopted for separation so as to distinguish the difference of the fragments of 20 bp. According to the invention, a tail sequence of 30bp or above is added to the 5' end of each primer, so that the fragment size difference between a wild type and a mutant after enzyme digestion is increased to 50bp or above.

The invention relates to paenibacillus polymyxa for controlling puccinia triticina of wheat and an application of the paenibacillus polymyxa and belongs to the technical field of microbial pesticides.The bacterial strain paenibacillus polymyxa is preserved in CGMCC (China General Microbiological Culture Collection Center) with preservation number being CGMCC No.14879 on November 14, 2017. An antibiological inoculant for controlling puccinia recondite of wheat can be prepared from the paenibacillus polymyxa, infection of puccinia recondite of wheat can be controlled effectively, the effect isremarkable, meanwhile, plant growth can be promoted, and the paenibacillus polymyxa has good application prospect in the aspect of control for puccinia recondite of wheat.

The invention provides a molecular marker for detecting a wheat leaf rust resistance gene Lr47, a detection method and application of the molecular marker. The invention develops a molecular marker Pku1176 closely linked with a leaf rust resistant gene Lr47 by means of an induced wheat-Aegilops spelltuifolia leaf rust resistant short fragment translocation line and in combination with reference sequences of wheat and Aegilops spelltuifolia. By utilizing the molecular marker, translocated short fragment 7S chromosomes under different wheat backgrounds can be accurately and quickly detected, the early molecular assisted selection of the leaf rust resistant gene Lr47 is realized, and the wheat disease-resistant breeding efficiency is improved. The method solves the problem of high difficulty in detecting the wheat rust-resistant gene in the prior art, and is suitable for the field of molecular genetic breeding.