27 results about "Wheat leaf rust" patented technology

The invention provides a functional molecular marker of a wheat leaf rust resistance gene Lr.ace-4A, a detection method and application of the functional molecular marker. The functional molecular marker comprises an upstream primer as shown in SEQ ID NO: 1 and a downstream primer as shown in SEQ ID NO: 2. The method can solve the problem that the Lr.ace-4A gene in wheat is difficult to rapidly detect in the prior art, and is suitable for the field of molecular genetic breeding.

The invention provides a wheat leaf rust resistance protein, a wheat leaf rust resistance gene and application thereof. Wherein the wheat leaf rust resistance protein comprises a DUF1 protein and / or a DUF2 protein, and the DUF1 protein comprises a protein composed of an amino acid sequence as shown in SEQ ID NO: 3 or a protein with 80% or more of homology and leaf rust resistance; the DUF2 protein comprises a protein composed of an amino acid sequence as shown in SEQ ID NO: 5 or a protein with 80% or more of homology and leaf rust resistance. The method can solve the problem of lack of leaf rust resistant protein in the prior art, and is suitable for the field of wheat breeding.

The present application relates to the technical field of biomolecule detection, in particular to a molecular marker related to leaf rust resistance, a primer pair and application thereof. The present application uses 559 materials to perform whole genome association analysis on the resistance of leaf rust in three environments, and finds 1 SNP closely related to leaf rust on the short arm of 2B chromosome of hexaploid wheat. A molecular marker capable of large-scale screening of materials is developed using the SNP, and the close correlation with leaf rust resistance is verified using 414 materials, and finally the molecular marker provided by the present application is developed. The development of the molecular marker provides a simple and effective new screening marker for wheat leaf rust resistance breeding. Using the molecular marker linked to the resistance locus QLr.hazu-2BS.1, the disease-resistant wheat strains can be quickly and accurately screened, the breeding period and experimental cost are saved, and the wheat resistance breeding process is accelerated.

The application discloses a wheat leaf rust resistance linkage SNP site, a dCAPS molecular marker, a primer, a kit and application, and belongs to the technical field of wheat disease resistance breeding and molecular biology. The application provides a wheat leaf rust resistance linkage SNP site, wherein the SNP site is located at the 134261400th base on the 4D chromosome of a Chinese spring reference sequence with the version number of IWGSC_v1.1, and the polymorphism site is T / G. With the aid of 277 wheat materials introduced from Turkey with genotype data, and in combination with the Chinese spring reference sequence, a dCAPS molecular marker of the SNP site linked to the wheat adult stage leaf rust resistance genetic locus is developed, different wheat can be accurately and rapidly detected, early molecular assisted selection of the adult stage leaf rust resistance germplasm material is realized, and the efficiency of wheat disease resistance breeding is improved.

The invention belongs to the technical field of agricultural bioengineering, and relates to application of a wheat disease-resistant factor TaPKS26 in prevention and treatment of leaf rust. The CDS sequence of the encoding gene of the wheat disease-resistant factor TaPKS26 is shown as SEQ ID NO: 1, a transgenic wheat strain of the overexpressed gene TaPKS26 is obtained by adopting an agrobacterium-mediated method, and the transgenic wheat strain of the overexpressed gene TaPKS26 is proved to have enhanced resistance to wheat leaf rust compared with Fielder wheat after being infected with physiological races THT and PHT, and the wheat leaf rust resistance is improved. The expression quantity of the disease-resistant related marker gene is up-regulated, which indicates that the wheat disease-resistant factor TaPKS26 plays a positive regulation role in wheat leaf rust-resistant defensive reaction. The invention provides new gene resources and technical thoughts for the cultivation of leaf rust resistant varieties.

The present invention discloses a wheat leaf rust resistance molecular marker and its application. The wheat leaf rust resistance molecular marker disclosed in the present invention is a nucleotide at position 71 on wheat chromosome 5B corresponding to SEQ ID No. 4 in the sequence list, which is C or T. Experiments have shown that the wheat leaf rust resistance molecular marker of the present invention is associated with wheat leaf rust resistance. Homozygous wheat in which the nucleotide at position 71 in genomic DNA corresponding to SEQ ID No. 4 in the sequence list is C has higher leaf rust resistance than homozygous wheat in which the nucleotide at position 71 in genomic DNA corresponding to SEQ ID No. 4 in the sequence list is T. The wheat leaf rust resistance molecular marker of the present invention can be used to detect wheat leaf rust resistance and further used in molecular marker-assisted breeding. The present invention is of great significance for breeding wheat with enhanced leaf rust resistance.

The invention discloses a wheat leaf rust resistance QTL and application of a KASP marker thereof. The invention belongs to the technical field of biology, and particularly relates to wheat leaf rust resistance QTL and application of a KASP marker of the wheat leaf rust resistance QTL. The substance for detecting the polymorphism or genotype of the SNP in the wheat genome can be applied to (1) identification or auxiliary identification of wheat leaf rust resistance; (2) wheat breeding; (3) preparing a product for identification or auxiliary identification of wheat leaf rust resistance; (4) preparing a wheat breeding product; the SNP site is located on a wheat 2A chromosome, and the nucleotide of the SNP site is A or G and is the 193rd nucleotide of a sequence 4 in a sequence table. The KASP labeled KASP-LR-2AL and the specific KASP primer group thereof developed by the invention can be used for molecular marker-assisted screening, and have important significance for cultivating wheat varieties with enhanced leaf rust antibodies.

The invention discloses a molecular marker for detecting a leaf rust resistant gene Lr19 and application of the molecular marker, and belongs to the technical field of wheat molecular breeding. The molecular marker disclosed by the invention is at least one of a molecular marker Lsdau-7 and a molecular marker YXsdau-9; the nucleotide sequence of the molecular marker Lsdau-7 is as shown in SEQ ID NO. 1; the nucleotide sequence of the molecular marker YXsdau-9 is as shown in SEQ ID NO. 2. By utilizing the molecular marker disclosed by the invention, the specific detection of the leaf rust resistant gene Lr19 can be realized, and the leaf rust resistant breeding efficiency of wheat is improved.

The present invention provides a wheat leaf rust resistance protein Lr.ace-4A, a resistance gene, and an application. The wheat leaf rust resistance protein Lr.ace-4A comprises: (a) a protein having an amino acid sequence as shown in SEQ ID NO: 3; or (b) a protein having wheat leaf rust resistance activity after substitution and / or deletion and / or addition of one or more amino acids in the amino acid sequence in (a); or (c) a protein having more than 80% homology with the amino acid sequence defined in either (a) or (b) and having the same function. The present invention can solve the problem of the lack of leaf rust resistance proteins in the prior art and is applicable to the field of wheat breeding.

The invention relates to wheat puccinia tritici effect protein Pt26886 as well as a coding gene and application thereof, and belongs to the technical field of biology. The invention provides a protein with an amino acid sequence as shown in SEQ ID NO: 2, a nucleic acid molecule for coding the protein, and an action mechanism of the protein or the nucleic acid molecule in regulating the resistance of wheat to wheat leaf rust. The invention is helpful for understanding the interaction mechanism between the effector protein and the host, and provides a molecular basis for clarification of the pathogenic mechanism of pathogenic bacteria.

The present application relates to the application of wheat AvrLr15 gene, primer set and kit in detecting and / or identifying the virulence of wheat leaf rust fungus, and belongs to the technical field of pathogenic microorganism detection.The present application provides the application of wheat AvrLr15 gene in detecting and / or identifying the virulence of wheat leaf rust fungus, wherein the CDS region nucleotide sequence of the AvrLr15 gene is shown as SEQ ID NO.1.The primer set of the present application can directly detect and / or identify the virulence of wheat leaf rust fungus to the wheat leaf rust resistant material TcLr15, and has good specificity, reliability and stability, and can be used as a primer set of specific molecular marker for detecting and / or identifying the virulence of wheat leaf rust fungus to TcLr15.

The invention relates to application of a wheat AvrLr15 gene, a primer group and a kit to detection and / or identification of wheat puccinia puccinia virulence, and belongs to the technical field of pathogenic microorganism detection. The invention provides an application of a wheat AvrLr15 gene in detecting and / or identifying the toxicity of puccinia tritici. The nucleotide sequence of a CDS region of the AvrLr15 gene is as shown in SEQ ID NO. 1. The primer group provided by the invention can directly detect and / or identify the toxicity of puccinia tritici to a wheat leaf rust resistant material TcLr15, has good specificity, reliability and stability, and can be used as a specific molecular marker primer group for detecting and / or identifying the toxicity of puccinia tritici to TcLr15.

The invention provides a wheat leaf rust resistance protein Lr.ace-4A, a resistance gene and application of the wheat leaf rust resistance protein Lr.ace-4A and the resistance gene. The wheat leaf rust resistance protein Lr.ace-4A comprises: (a) a protein composed of an amino acid sequence represented by SEQ ID NO: 3; or (b) a protein which is obtained by substitution and / or deletion and / or addition of one or more amino acids in the amino acid sequence in (a) and has wheat leaf rust resistance activity; or (c) protein which has more than 80% of homology with the amino acid sequence limited by any one of (a) and (b) and has the same function. The method can solve the problem of lack of leaf rust resistant protein in the prior art, and is suitable for the field of wheat breeding.

A wheat leaf rust resistance protein, its encoding gene, and the use thereof are provided. The wheat leaf rust resistance protein includes any of the following (a)-(c): (a) a protein, which consists of the amino acid sequence represented by SEQ ID NO: 3; or (b) a protein, which has an amino acid sequence obtained after the amino acid sequence of the protein (a) undergoes substitution and / or deletion and / or addition of one or more amino acids and which has anti-wheat-leaf-rust activity; or (c) a protein, which has 80% or more identity with the amino acid sequence defined in any of (a) and (b) and which has the same function.

The present application belongs to the technical field of agricultural bioengineering, and relates to application of a wheat disease resistance factor TaPKS26 in leaf rust prevention and treatment. The CDS sequence of the coding gene of the wheat disease resistance factor TaPKS26 is shown as SEQ ID NO:1, and the present application adopts an agrobacterium-mediated method to obtain a transgenic wheat strain overexpressing the gene TaPKS26. After being infected with the physiological race THT and PHT of leaf rust, the transgenic wheat strain overexpressing the gene TaPKS26 is compared with Fielder wheat, and the results show that the resistance of the transgenic wheat strain to wheat leaf rust is enhanced, and the expression amount of a disease resistance related marker gene is up-regulated, indicating that the wheat disease resistance factor TaPKS26 plays a positive regulation role in the defense response of wheat against leaf rust. The present application provides a new gene resource and technical idea for breeding of a leaf rust disease resistance variety.

The application provides a functional molecular marker of a wheat leaf rust resistance gene Lr.ace-4A, a detection method and application thereof. The functional molecular marker comprises an upstream primer shown in SEQ ID NO:1 and a downstream primer shown in SEQ ID NO:2. The application can solve the problem that the Lr.ace-4A gene in the wheat is difficult to detect rapidly in the prior art, and is suitable for the field of molecular genetic breeding.

This invention relates to the field of genetic engineering technology, and more particularly to a molecular marker for detecting wheat leaf rust resistance genes LrN4B or LrN3B and its application. The molecular marker LrN4B-1 is located in the 578-585Mb region of chromosome 4BL, with a polymorphism of C / G. The molecular marker LrN3B-1 is located in the 4-8Mb region of chromosome 3BS, with a polymorphism of T / C. This invention obtains molecular markers linked to wheat leaf rust resistance genes LrN4B and LrN3B through the construction of genetic linkage maps and the screening and localization of molecular markers. These two molecular markers can be used to distinguish the LrN4B+LrN3B disease-resistant genotype, screen for wheat lines containing these two disease-resistant genes, accelerate the process of wheat disease-resistant breeding, and are of great significance in the field of breeding leaf rust-resistant wheat.

The invention discloses a wheat 14-3-3 protein with resistance to wheat leaf rust and a coding gene thereof, provides application of the protein and the gene in improving or weakening the wheat leaf rust, and also discloses a method for cultivating a wheat leaf rust resistant plant. A new material and a new way are provided for effectively preventing and treating the wheat leaf rust, and rapid, effective and directional character improvement is achieved on the molecular level.

The application discloses a wheat E3 ubiquitin ligase gene RFEL1 and application thereof in wheat disease resistance, and belongs to the technical field of plant genetic engineering. The nucleotide sequence of the wheat E3 ubiquitin ligase gene RFEL1 is shown as SEQ ID NO. 3. The application also provides application of the wheat E3 ubiquitin ligase gene RFEL1 or related biological materials thereof, including application of any one of the following: A1, improving the resistance of wheat to stripe rust; A2, improving the resistance of wheat to powdery mildew; and A3, improving the resistance of wheat to leaf rust. The E3 ubiquitin ligase RFEL1 gene provided by the application not only has broad-spectrum resistance to different stripe rusts, but also has resistance to wheat leaf rust and powdery mildew. The E3 ubiquitin ligase RFEL1 gene of the application has important application value when used in wheat broad-spectrum resistance breeding.

The invention discloses a molecular marker of leaf rust resistance QTL QLr.lnfu-7BL2 and application of the molecular marker. The invention belongs to the technical field of biology, and particularly relates to a molecular marker of leaf rust resistance QTL QLr.lnfu-7BL2 and application of the molecular marker. The substance for detecting the polymorphism or genotype of the SNP in the wheat genome can be applied to (1) identification or auxiliary identification of wheat leaf rust resistance; (2) wheat breeding; (3) preparing a product for identification or auxiliary identification of wheat leaf rust resistance; (4) preparing a wheat breeding product; the SNP site is located on a wheat 7B chromosome, and the nucleotide of the SNP site is T or C and is the 83rd nucleotide of a sequence 4 in a sequence table. The KASP labeled KASP-LR-7BL. 2 and the specific KASP primer group of the KASP labeled KASP-LR-7BL. 2, which are developed by the invention, can be used for molecular marker-assisted screening, and are of great significance for cultivating wheat varieties with enhanced leaf rust antibodies.

The invention discloses application of a wheat disease-resistant factor TaC4H1 in prevention and treatment of brown leaf rust, and belongs to the technical field of genetic engineering. The coding gene CDS sequence of the wheat disease resistance factor TaC4H1 is as shown in SEQ ID NO: 1, and the amino acid sequence is as shown in SEQ ID NO: 2. According to the invention, a transgenic wheat strain capable of overexpressing TaC4H1 is obtained by adopting an agrobacterium-mediated method, and the condition that the wheat disease-resistant factor TaC4H1 plays a positive regulation role in wheat leaf rust resistance immune response is known by comparing the morbidity condition of transgenic wheat and wild Fielder wheat after being infected with physiological races of leaf rust THT and PHT and the expression condition of a disease-resistant related marker gene. On the basis, the wheat disease-resistant factor TaC4H1 can be used for creating a leaf rust-resistant wheat material, so that a gene resource and a technical thought are provided for leaf rust-resistant breeding of wheat.

The invention discloses a novel thinopyrum triticum translocation line detection method and application thereof. According to the method, specific markers CINAU1181 (SEQ ID NO.17-18) and CINAU1381 (SEQ ID NO.19-20) of thinopyrum triticum are expanded through PCR (Polymerase Chain Reaction), and a translocation line is judged according to a 253bp / 254bp band. The marker and the leaf rust resistant gene LrSN21044 are co-separated (the co-separation rate is greater than or equal to 98%). The method can be applied to wheat genetic improvement, and is especially suitable for rapid breeding of wheat leaf rust resistant varieties.

The invention discloses a wheat leaf rust resistance linkage SNP site, a dCAPS molecular marker, a primer, a kit and application, and belongs to the technical field of wheat disease resistance breeding and molecular biology. The invention provides a wheat leaf rust resistance linked SNP (Single Nucleotide Polymorphism) site, which is positioned at the 134261400th basic group on a wheat Chinese spring reference sequence 4D chromosome with the version number of IWGSCv1.1, and the polymorphic site of the SNP site is T / G. By means of 277 parts of wheat materials which have genotype data and are introduced from Turkey and in combination with a Chinese spring reference sequence, the dCAPS molecular marker of the SNP linked with the leaf rust resistant genetic locus in the wheat adult-plant stage is developed, different wheat can be accurately and rapidly detected, early molecular assisted selection of leaf rust resistant germplasm materials in the adult-plant stage is achieved, and the application prospect is wide. The wheat disease-resistant breeding efficiency is improved.

The invention belongs to the technical field of plant disease prevention and treatment, and particularly relates to application of 2-methylglutaric acid in inhibition of wheat leaf rust and prevention and treatment of wheat leaf rust. The 2-methylglutaric acid has an obvious inhibition effect on wheat leaf rust, the attack level of the leaf rust can be reduced after the 2-methylglutaric acid is sprayed on wheat, and meanwhile, the invention provides application of the regulation and control method in prevention and control of wheat diseases. According to the invention, the internal mechanism of wheat leaf rust resistance reaction is deeply explored based on qualitative and quantitative analysis of metabolites by comprehensively analyzing the change of metabolites when wheat is infected by leaf rust. In the process, it is found that the metabolite 2-methylglutaric acid has an obvious inhibition effect on the germination process of puccinia tritici spores and can be used as a novel compound for preventing and treating the puccinia tritici.

The present invention discloses molecular markers XsdauLH3388 and Xsdau19A20A for the wheat leaf rust resistance gene Lr29 and their applications. The nucleotide sequence of the molecular marker XsdauLH3388 of the present invention is shown in SEQ ID NO.1, which is a co-dominant InDel marker; the nucleotide sequence of the molecular marker Xsdau19A20A is shown in SEQ ID NO.3, which is a co-dominant CAPS marker. The molecular markers XsdauLH3388 or Xsdau19A20A can be used to detect the wheat leaf rust resistance gene Lr29 and the genotype of plants containing the Lr29 gene. Therefore, the molecular markers of the present invention can be used for molecular marker-assisted selection breeding of leaf rust resistance wheat, accelerating the wheat breeding process and being suitable for large-scale promotion and application.

The invention belongs to the field of wheat genetic breeding, and discloses a creation method and application of a wheat-elytrigia elongata leaf rust resistant short fragment translocation line. The invention further discloses a creation method of the wheat-agropyron elongatum leaf rust resistant short-fragment genetic improvement line and the molecular marker K-1001 closely linked with the wheat leaf rust resistant gene Lr19 in the wheat-agropyron elongatum leaf rust resistant short-fragment translocation line. According to the invention, molecular marker-assisted selection, an in-situ hybridization identification technology and brown leaf rust resistance identification are combined, linkage of Lr19 and PSY-E1 is broken, the translocation line not only keeps stable brown leaf rust resistance, but also the flour is white in color, and can be better popularized and applied in production.

The utility model relates to the technical field of sample storage devices, and discloses a wheat leaf rust original sample storage device which comprises a base, a storage box is fixedly installed at the top of the base, a temperature and humidity controller is arranged on the front face of the storage box, and a temperature and humidity control mechanism is arranged in the storage box and extends to the outside. The temperature and humidity control mechanism comprises a temperature adjusting assembly, a humidifying assembly and an exhaust assembly, the temperature adjusting assembly is arranged outside the left side of the storage box, the humidifying assembly is arranged inside the base, and the exhaust assembly is arranged outside the right side of the storage box. According to the wheat leaf rust original sample storage device, by arranging the temperature and humidity control mechanism and the temperature and humidity controller, in the using process, the temperature and humidity in the storage box can be controlled and adjusted, the fixed temperature and humidity in the storage box are maintained, and therefore it is guaranteed that wheat leaf rust original samples are in the optimal storage state; the preservation time of the wheat leaf rust original sample is prolonged.