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Antigens and polyclonal antibodies for differentiating between wheat leaf rust and bacillus rust

A technology of polyclonal antibody and wheat leaf rust, which is applied in the preparation method of peptides, chemical instruments and methods, anti-fungal/algae/lichen immunoglobulin, etc., can solve the problem of difficult observation of host symptoms, time-consuming and labor-consuming, It is difficult to meet the problems of rapid and high-throughput diagnostic testing, and achieve the effect of simple and effective early infection and easy operation

Active Publication Date: 2018-11-23
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

Not only is it time-consuming and labor-intensive, but the accuracy and reliability of the results also depend to a large extent on the technical level and experience of the surveyors, making it difficult to meet the actual needs of rapid, high-throughput diagnostic testing
Especially in the incubation stage of wheat leaf rust, the symptoms of the host are not easy to observe, and it is difficult to define the period and scale of the initial infection

Method used

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  • Antigens and polyclonal antibodies for differentiating between wheat leaf rust and bacillus rust
  • Antigens and polyclonal antibodies for differentiating between wheat leaf rust and bacillus rust
  • Antigens and polyclonal antibodies for differentiating between wheat leaf rust and bacillus rust

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Embodiment 1

[0029] Example 1. Preparation of polyclonal antibodies for identifying bacillus rust

[0030] Reagents and solutions:

[0031] Freund's complete adjuvant, Freund's incomplete adjuvant (Beijing Qinbang Biotechnology Co., Ltd.)

[0032] Phosphate buffered saline (PBS, 0.02M, pH7.2),

[0033] Coating solution (phosphate buffer, 0.05M, pH7.4),

[0034] Reaction Diluent (PBST, pH7.4, 0.03M, +0.05% Tween 20),

[0035] Washing working solution (dilute the 20× concentrated washing solution with deionized water at a volume ratio of 1:19);

[0036] Blocking solution (add 0.05% BSA in 0.02mol / L PBST),

[0037] Substrate chromogenic solution: composed of liquid A and liquid B, liquid A is hydrogen peroxide, liquid B is tetramethylbenzidine;

[0038] Stop solution (2mol / L H 2 SO 4 ),

[0039] Enzyme-labeled secondary antibody (Beijing Qinbang Biotechnology): horseradish peroxidase-labeled goat anti-rabbit IGg antibody

[0040] 4 kinds of rust fungi shown in Table 1, the suspension...

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Abstract

The invention relates to an antigen and a polyclonal antibody for identifying wheat puccinia recondita and wheat puccinia graminis. An antigen polypeptide for preparation of the polyclonal antibody for distinguishing wheat puccinia recondita and wheat puccinia graminis has an amino acid sequence shown in SEQ ID NO:1. The immunogen for preparation of the polyclonal antibody for distinguishing wheat puccinia recondita and wheat puccinia graminis is characterized in that the immunogen is prepared from the antigen polypeptide as a hapten. The polyclonal antibody for distinguishing wheat puccinia recondita and wheat puccinia graminis is characterized in that domestic rabbits are immunized by the immunogen, and a high-titer immunized domestic rabbit is detected and screened; and whole blood of the selected domestic rabbit is taken, and the polyclonal antibody is obtained through purification. The method for distinguishing wheat puccinia recondita and wheat puccinia graminis can simply and effectively detect whether wheat suffers from early infection of puccinia recondita.

Description

technical field [0001] The invention relates to an antigen and a polyclonal antibody for distinguishing wheat leaf rust fungus and bacillus rust fungus, and belongs to the field of plant disease control. Background technique [0002] Wheat rust (PucciniareconditaRob.exDesm.f.sp.triticiErikss.Et Henn.) is a fungal disease caused by wheat rust, which is an important disease in China and all wheat-producing countries in the world. Yield loss of more than 15%. [0003] For a long time, the prediction of wheat rust has been mainly based on regular and large-scale field investigations and the isolation, cultivation and identification of indoor pathogen races. Not only is it time-consuming and labor-intensive, but the accuracy and reliability of the results also depend to a large extent on the technical level and experience of the surveyors, making it difficult to meet the actual needs of rapid, high-throughput diagnostic testing. Especially in the incubation stage of wheat leaf ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/37C07K19/00C07K1/107C07K16/14C07K16/06G01N33/569
CPCC07K14/37C07K14/765C07K16/06C07K16/14C07K2319/31G01N33/56961G01N2469/10
Inventor 高利沈慧敏李超陈万权刘太国刘博
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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