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The application discloses an application of an animal domestication behavior related RRM1 gene mutant. Through population genetics, comparative genomics and ancient genomics analysis, the cause variation of the strongest selected gene of goat domestication, namely, the RRM1 gene c.1020A>G mutation, is determined; through association analysis with a hybrid population with wild goat blood, it is found that the mutation is significantly related to the domestication behavior in the goat domestication process; and through construction of a mouse containing the RRM1 gene c.1020A>G mutation, it is proved that the corresponding mutant of the RRM1 gene obviously increases the domestication behavior of the mouse. The application provides a basis and lays a foundation for phenotype selection of the domestication behavior and preparation of a tame animal model.