41 results about "European rabbit" patented technology

The invention is applicable to the technical field of rabbit breeding, and provides a method for increasing the body weight of a rabbit based on a gene editing technology, which comprises the following steps: designing an action target sgRNA on a rabbit NR6A1 gene aiming at an NR6A1 p.P192S site; uniformly mixing the synthesized sgRNA and SpRY-CBE, injecting fertilized eggs, transplanting the fertilized eggs into fallopian tubes of receptor female rabbits, and feeding the receptor female rabbits until production; dNA is extracted from produced young rabbit ear tissues, PCR and sequencing are carried out, and if C at the fifth site in NR6A1 gene target sites is mutated into T, it is proved that single base mutation is obtained, and the NR6A1 gene edited rabbit is obtained. According to the invention, the rabbit variety with increased weight is successfully cultivated; a new thought of animal genetic breeding is developed by utilizing a gene editing technology, the method has application values in promoting improvement of genetic characters of rabbits and improving production performance and economic value of the rabbits, and powerful support is provided for high-quality development of the animal breeding industry in China.

The invention discloses a feed containing astragalus polysaccharide and a preparation method thereof, and belongs to the field of feeds. The astragalus polysaccharide is added into the basic feed, so that the adverse effect of heat stress on the female rabbits can be effectively relieved, the conception rate of the female rabbits, the number of live litters and the average weight of the live litters can be increased, and the breeding economic benefits are improved while the reproductive performance is optimized and the growth of the young rabbits is promoted; the prepared feed can effectively improve the serum hormone level of animals and reduce the inflammatory factor level, meanwhile, the diversity of animal intestinal microorganisms under heat stress is effectively improved, a scientific basis is provided for precise feeding management of domestic rabbits in summer, and meanwhile, an important reference is provided for application of astragalus polysaccharide in the field of livestock and poultry anti-stress.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to rabbit feed conversion rate and application of the SNP molecular marker, and belongs to the technical field of molecular marker-assisted breeding. The SNP site is located at the 10085164bp position of a chromosome 5 of a reference genome of the Kangbig meat rabbit, and the polymorphism of the SNP site is T / C. According to the SNP locus and the detection method provided by the invention, an efficient and accurate molecular marker-assisted breeding technology can be established, and when the molecular marker-assisted breeding technology is applied to selection of the feed conversion rate of breeding rabbits, the feed conversion rate and the breeding benefits of the rabbits can be genetically improved.

The utility model relates to the technical field of rabbit cages, and discloses a transfer box for rabbit breeding, which comprises a rabbit cage body, and the rabbit cage body comprises a base and a cage body hinged on the base; a detachable grid plate matched with the base is arranged at the bottom of the cage body, and a screen cover body is hinged to the top of the cage body; a movable water feeder is arranged on the side wall of the cage body; the water feeder comprises a stud rotationally arranged in a hole of the cage body, a nut is arranged at the end, located outside the cage body, of the stud, a water storage bottle is fixedly arranged at the end, located in the cage body, of the stud, the bottom of the water storage bottle is communicated with a pipeline, the end, away from the water storage bottle, of the pipeline is in an inverted cone shape, a rod body is arranged in the pipeline, and a spring is arranged on the rod body. The other end of the spring is provided with a ball which abuts against the water outlet of the pipeline for sealing. Compared with the prior art, the domestic rabbit transfer box has the advantages that excrement of domestic rabbits is convenient to clean, and the stability is good when the transfer box is stacked.

The invention discloses a rabbit lower limb pain assessment instrument, and relates to the technical field of pain assessment, the instrument comprises a bottom plate, and also comprises mounting plates fixedly arranged on two sides of the bottom plate, the inner side walls of the mounting plates are fixedly connected with a supporting plate, the supporting plate is of an L-shaped structure, and a limiting assembly is arranged above the supporting plate; a left balance and a right balance are arranged on the two sides of the bottom plate below the supporting plate respectively, and balance trays are arranged on the left balance and the right balance; the limiting assembly comprises mounting blocks fixedly arranged on the front side and the rear side of the supporting plate, limiting coamings are rotationally connected between the mounting blocks, a baffle is arranged between the limiting coamings, and the two sides of the baffle are slidably connected with the limiting coamings so that the size of the limiting space can be adjusted to adapt to the domestic rabbits of different body types; the rabbit lower limb pain assessment instrument has the remarkable advantages of being accurate in assessment, objective in quantification, easy and convenient to operate, low in cost, wide in applicability and the like, and a novel and effective assessment tool is provided for the field of pain research.

The application provides a fusion protein, a rabbit hemorrhagic disease virus type 2 self-assembled nanoparticle vaccine and application, and belongs to the technical field of biological medicine. The application provides a fusion protein, which comprises a first polypeptide, a hinge region of a rabbit hemorrhagic disease virus type 2 capsid protein, a P region of a rabbit hemorrhagic disease virus capsid protein and a second polypeptide connected in sequence; the first polypeptide and the second polypeptide are polypeptides containing four cysteines respectively. The fusion protein RGD4C-HP-RGD4C of the application can self-assemble to form nanoparticles, and the particle size is 40-50 nm. The fusion protein of the application can induce domestic rabbits to produce specific antibodies against RHDV2, produce a strong humoral immune response, and provide complete protection for the domestic rabbits.

The invention discloses a method for constructing a urinary tract infection domestic rabbit model, which comprises the following steps of: on the basis of catheter guidance, placing a ureteral catheter into a bladder through a urethra to manufacture a barrier-free artificial channel, then placing a spiral epidural catheter into the bladder through a ureteral catheter channel, and finally, placing the spiral epidural catheter into the bladder through the ureteral catheter channel. After Escherichia coli is injected through a ureteral catheter, a urethral orifice is quickly ligated with a silk thread, and the urethra is reopened after 2 hours. According to the invention, the condition of complicated urinary tract infection caused by common clinical calculi, foreign matter retention (stent tubes, ureters and the like) and the like can be simulated; compared with a ureter semi-ligation model, the method is relatively simple to operate, low in infection rate and high in repeatability; compared with a bladder direct injection bacterial model without laparotomy, the model has the advantages of typical pathological process and high and stable urinary tract infection success rate.

The invention relates to a molecular marker related to rabbit fur weight and application thereof, and belongs to the technical field of rabbit molecular marker breeding. The molecular marker is SNP (Single Nucleotide Polymorphism) and is located at 1229288bp of a chromosome 1 of a reference genome of a Kangbig meat rabbit, the polymorphism of the molecular marker is A / G, and the genotype of the site is that the fur weight of a GG rabbit individual is maximum. According to the SNP site and the detection method provided by the invention, a molecular marker-assisted breeding technology for rabbit fur traits can be established, and the molecular marker-assisted breeding technology is used for genetically improving the fur weight and quality of rabbits and improving the rabbit fur value and breeding benefits.

The invention provides a fusion protein, a rabbit hemorrhagic disease virus type 2 self-assembled nanoparticle vaccine and application, and belongs to the technical field of biological medicine. The invention provides a fusion protein. The fusion protein comprises a first polypeptide, a hinge region of a rabbit hemorrhagic disease virus type 2 capsid protein, a P region of the rabbit hemorrhagic disease virus capsid protein and a second polypeptide which are connected in sequence, the first polypeptide and the second polypeptide are respectively polypeptide containing four cysteines. The fusion protein RGD4C-HP-RGD4C disclosed by the invention can be self-assembled to form nano particles, and the particle size is 40 to 50 nm. The fusion protein provided by the invention can induce a rabbit to generate a specific antibody aiming at RHDV2, generate a relatively strong humoral immune response and provide complete protection for the rabbit.

The invention discloses a domestic rabbit whole genome multifunctional chip and application, and belongs to the technical field of molecular biology. The invention relates to a rabbit whole genome 10K liquid phase breeding chip based on a target area genome sequence liquid phase capture accurate positioning sequencing typing technology. The chip comprises 10331 SNP (Single Nucleotide Polymorphism) loci. The chip can be applied to rabbit genetic diversity evaluation, germplasm resource and genetic relationship identification, important economic character whole genome association analysis and whole genome selective breeding, has the advantages of high throughput, high interval coverage rate, high locus detection rate, high flexibility and the like, and provides powerful tool support for rabbit molecular breeding and variety identification.

The utility model discloses a fixing device for a domestic rabbit experiment, which comprises a box body, a cover body matched with the box body is arranged on the box body, one side of the box body is fixedly connected with one side of the cover body through a connecting assembly, the other side of the box body is connected with the other side of the cover body through a quick lock catch, a first rabbit neck groove is arranged at the end part of the box body, and a second rabbit neck groove is arranged at the end part of the box body. A second rabbit neck groove matched with the first rabbit neck groove is formed in the end of the cover body, a sliding plate matched with the second rabbit neck groove is arranged at the end of the cover body in a sliding mode, a third rabbit neck groove matched with the first rabbit neck groove is formed in the sliding plate, a guide locking block is fixedly arranged on the sliding plate, and a guide groove matched with the guide locking block is formed in the cover body. An adjusting assembly is arranged between the cover body and the sliding plate. One side of the box body is connected with one side of the cover body through the connecting assembly, and the other side of the box body is connected with the other side of the cover body through the quick lock catch, so that the rabbit can be taken and placed more conveniently and quickly in the experiment process without being affected, and the size of the neck of the rabbit is adjusted through the sliding plate, so that the operation is more convenient.

The utility model belongs to the technical field of instruments for animal experiments, discloses a rabbit ear edge intravenous injection fixing device, and aims to solve the problems that in the daily operation process, due to struggling of a domestic rabbit body and emergency response of rabbit ears, the rabbit body rolls and shakes, an injection needle easily penetrates through the rabbit ears, and fingers of an operator are injured. The technical scheme of the utility model is as follows: the experiment table comprises a working table, a lighting device is fixedly arranged on the top surface of the working table, a base is arranged on the top surface of the working table, vertical plates which are uniformly distributed are fixedly arranged on the top surface of the base, and the vertical plates are fixedly arranged on the top surface of the working table. According to the rabbit ear edge intravenous injection device, a rabbit can be fixed through the front plate, the inserting plate and the fixed plate, rabbit ears and the movable plate can be fixed through the binding device, the movable plate can move along the transverse shaft, the position angle of the rabbit ears placed on the movable plate can be adjusted conveniently, and an operator can conduct rabbit ear edge intravenous injection operation conveniently; the device can be operated by one person and is simple and convenient to use.

The invention is applicable to the technical field of animal model construction, and provides a method for constructing a rabbit growth trait model based on ZDHHC23 gene knockout, and the method comprises the following steps: transcription synthesis of SpG-Cas9 mRNA; the method comprises the following steps: designing and synthesizing a zinc finger structural domain sgRNA of a rabbit ZDHHC23, namely designing an action target sgRNA on a rabbit ZDHHC23 gene aiming at the zinc finger structural domain of the rabbit ZDHHC23; and preparing the ZDHHC23 gene edited rabbit by an embryo microinjection technology. According to the embodiment of the invention, a SpG-Cas9 gene editing technology is utilized, gene knockout is carried out on the zinc finger structural domain of the rabbit ZDHHC23, a new variety of the rabbit with increased weight is obtained, and the method has application value in promoting improvement of genetic characters of the rabbit and improving production performance and economic value of the rabbit.

The utility model relates to a domestic rabbit ear edge venous transfusion fixing device which comprises a fixing box, a first clamping plate is fixedly installed inside the fixing box, a second clamping plate is hinged to the outer portion of the first clamping plate, a spring is fixedly installed inside the second clamping plate, a movable plate is fixedly installed outside the spring, and the movable plate is hinged to the outer portion of the first clamping plate. A bolt is fixedly installed outside the first clamping plate, a threaded rod is connected to the interior of the bolt in a threaded mode, and a clamping piece is fixedly installed outside the movable plate. According to the rabbit ear edge venous transfusion fixing device, a rabbit is placed in the fixing box, the body of the rabbit is fixed through a first elastic belt, four limbs of the rabbit are fixed through a second elastic belt, two ears of the rabbit penetrate through a partition plate and reach the top of a first clamping plate, and the first clamping plate is attached to a second clamping plate; the ear is limited, and the ear is fixed through the movable plate, so that instability of intravenous injection caused by disordered movement of the ear is prevented.

The utility model relates to the technical field of medical teaching, and discloses an auxiliary device for preparing a rabbit hemorrhagic shock model. Bloodletting and rabbit blood pressure monitoring of rabbits can be realized through one-time neck artery intubation. On one hand, by adjusting the height of the blood storage bottle and the height of the transducer, the position of the rabbit heart and the height of the transducer are horizontal, so that the experiment accuracy can be guaranteed, on the other hand, the height difference between the liquid level in the blood storage bottle and the position of the rabbit heart is adjusted to be 55 cm, and the blood pressure of the rabbit can be maintained at 40 mmHg; the bloodletting amount can be sensitively and effectively controlled, and even if the bloodletting amount exceeds the standard in a short time due to improper operation, blood in the blood storage bottle can flow back to the rabbit artery by reducing the height of the blood storage bottle, so that the experiment failure caused by excessive bloodletting amount is prevented; and the reading of the signal acquisition device and the height of the liquid level in the blood storage bottle in the experiment can be subjected to bidirectional comparison and bidirectional verification, so that the accuracy of corresponding data of the experiment is further ensured.

The present invention is applicable to the field of rabbit breeding technology and provides a method for increasing rabbit weight based on gene editing technology, comprising: designing a target sgRNA for the NR6A1 p.P192S site on the rabbit NR6A1 gene; mixing the synthesized sgRNA and SpRY‑CBE, injecting the resultant sgRNA into a fertilized egg, transplanting the mixture into the oviduct of a recipient female rabbit, and raising the recipient female rabbit until birth; extracting DNA from the ear tissue of the resulting young rabbit, performing PCR and sequencing, and if the C at position 5 in the NR6A1 gene target site mutates to T, a single base mutation is obtained, i.e., an NR6A1 gene-edited rabbit is obtained. The present invention successfully breeds a rabbit breed with increased weight; utilizing gene editing technology to develop new ideas for animal genetic breeding, has application value in promoting the improvement of rabbit genetic traits, enhancing rabbit production performance and economic value, and provides strong support for the high-quality development of my country's animal seed industry.

The invention relates to a molecular marker related to tenderness of rabbit meat and application of the molecular marker, and belongs to the technical field of rabbit molecular marker breeding. The molecular marker is SNP (Single Nucleotide Polymorphism) and is located at 77978399bp of chromosome 11 of a reference genome of a Kangbig meat rabbit, the polymorphism of the molecular marker is C / T, and the tenderness of the rabbit meat of an individual with the genotype of TT or CT at the site is good. According to the SNP site and the detection method provided by the invention, a molecular marker-assisted breeding technology for tenderness of rabbit meat can be established, and the molecular marker-assisted breeding technology is applied to genetic improvement of tenderness of rabbit meat and improvement of rabbit meat quality and economic benefits.

The utility model relates to a domestic rabbit feed box which comprises a feed box body, a cleaning assembly is installed at the bottom end of the feed box body, the cleaning assembly comprises a barrier strip, guide rails are fixedly connected to the two ends of the barrier strip respectively, a scraping assembly is fixedly connected to the rear portion between the two guide rails, and reset assemblies are fixedly connected to the bottom ends of the guide rails. A flat plate is mounted between the mounting grooves in the two guide rails, first moving blocks are fixedly connected to the front portions of the two sides of the flat plate respectively, limiting blocks are fixedly connected to the rear portions of the inner sides of the mounting grooves in the guide rails, and a handle is fixedly connected to the rear portion of the middle of the bottom end of the flat plate. The domestic rabbit feed box has the effect that the feed layer deposited on the flat plate at the bottom end of the feed box body can be conveniently cleaned, the labor amount of workers is reduced, the deposited feed layer can be cleaned in time, and the deposited feed layer is prevented from mildewing to generate harmful substances which affect the health of domestic rabbits.

The utility model discloses a domestic rabbit experiment device with a cleaning function, and relates to the technical field of experiment tables. Comprising an experiment table, the bottom of which is provided with an insertion strip; the supporting mechanism is arranged below the experiment table and is used for supporting the experiment table; the receiving groove is of a square structure, and the size of the receiving groove is matched with that of the experiment table; the inserting groove is fixed to the inner side of the bearing groove, the inserting groove is of a [-shaped structure, the bearing groove is detachably installed on the inserting strip through the inserting groove, and the height of the top face of the bearing groove is smaller than that of the bottom face of the inserting strip; a discharge port is formed in the receiving groove, and the top end of the waste discharge pipe is communicated with the discharge port of the receiving groove. By arranging the bearing groove and other structures, during cleaning, the bearing groove can be used for collecting flowing liquid, the liquid is output through the waste discharge pipe, during the period, the annular filter plate can play a role in filtering, and practicability is improved; as the receiving groove is detachably connected with the insertion strip through the insertion groove, the use is flexible and convenient.

The invention relates to the technical field of vaccine preparation, in particular to a bivalent subunit vaccine based on rabbit hemorrhagic disease virus type 2 VLPs and pasteurella multocida OMV as well as a preparation method and application of the bivalent subunit vaccine based on the rabbit hemorrhagic disease virus type 2 VLPs and the pasteurella multocida OMV. The vaccine is obtained by combining type 2 rabbit hemorrhagic disease virus VLPs and pasteurella multocida OMV; the mass ratio of the type 2 rabbit hemorrhagic disease virus VLPs to the pasteurella multocida OMV is 1: 1 or 1: 2. The invention aims to construct a novel bivalent vaccine capable of preventing RHDV2 and Pm infection at the same time. Establishing a preparation process of VLPs of RHDV2 and OMV of Pm, and efficiently combining the VLPs and the OMV to form a bivalent subunit vaccine; the optimal immune dose and the scientific immune procedure are defined by performing immune test and challenge protection effect evaluation on the domestic rabbits. The research aims at providing a scientific basis for developing the RHDV2 and rabbit Pm bigeminy subunit vaccine, has industrial development potential and has important significance on high-quality development of the special rabbit industry.

The present disclosure relates to a method of obtaining a decellularized dermal matrix from a skin graft of a rabbit, hare or beaver, the method comprising the steps of: degreasing the skin graft using a hydrocarbon solvent, perchloroethylene, a modified alcohol or supercritical carbon dioxide, or a combination thereof; liming the degreased graft by immersing the graft in a solution comprising calcium hydroxide, sodium hydroxide, hydrogen peroxide, or a mixture thereof; fleshing the skin graft; optionally, de-ashing the skin graft; incubating the skin graft in an ethylenediamine tetraacetic acid solution; the skin graft is treated with the detergent solution for 30 minutes to 3 hours to obtain a decellularized dermal matrix. The present disclosure also relates to a decellularized dermal matrix obtained by said method; a composition comprising the decellularized dermal matrix for use in a medicament or veterinary medicament; and a dermal-epidermal skin substitute scaffold comprising the disclosed decellularized dermal matrix.

The invention discloses a rabbit semen preservation diluent containing serine and a preparation method, the diluent comprises the following components in percentage by weight: 2-4% of serine, 3-8% of glucose, 3-5% of yolk, 0.1-0.5% of citric acid, 0.2-0.5% of monopotassium phosphate, 0.2-0.5% of sodium bicarbonate, 0.01-0.1% of ethylene diamine tetraacetic acid (EDTA) and the balance of distilled water, so that the total volume reaches 100%. The method for prolonging the sperm preservation time comprises the steps that the concentration of serine is preferably 3%, the pH value is adjusted to 6.8-7.2, collected rabbit semen and a diluent are mixed according to the proportion of 1: 2-1: 5, and then the mixture is placed in a preservation environment at the temperature of 4 DEG C to be preserved. The method is characterized in that sperms can be preserved for 7-10 days, and the sperm motility is kept above 75% during the period. The invention can prolong the preservation time of the rabbit semen and keep the sperm motility. The diluent disclosed by the invention is beneficial to improving the fertilization rate and the breeding efficiency of rabbits.

This invention discloses a method for preparing a whole-body rabbit nerve specimen, comprising the following steps: S1, skinning and preservation: Skinning a rabbit carcass, separating the visceral nerves, removing the viscera, and then performing preservation treatment to obtain sample 1; S2, softening: Immersing sample 1 sequentially in a low-temperature softening enzyme EB solution, a mixed solution of hydrochloric acid and glycerol, and an alkaline solution to obtain sample 2; S3, nerve separation: Taking sample 2, removing muscle, fat, and bone, and separating the whole-body rabbit nerves; S4, defatting: Immersing the whole-body rabbit nerves sequentially in a sodium hydroxide solution and petroleum ether for defatting treatment to obtain sample 3; S5, bleaching and fixation: Bleaching sample 3 in hydrogen peroxide, then washing and drying to obtain sample 4; Fixing sample 4 onto a substrate in the upright posture of the rabbit to obtain the whole-body rabbit nerve specimen. This invention is simple to operate, and the resulting whole-body rabbit nerve specimen is easy to preserve and has a complete and aesthetically pleasing structure.

The invention relates to a molecular marker related to rabbit fur weight, a detection method and application thereof, and belongs to the technical field of rabbit molecular marker breeding. The molecular marker is SNP (Single Nucleotide Polymorphism) and is located at 1222295bp of a chromosome 1 of a reference genome of a Kangbig meat rabbit, the polymorphism of the molecular marker is A / T, and the weight of fur of an individual rabbit with the genotype of AA is maximum. According to the SNP site and the detection method provided by the invention, a molecular marker-assisted breeding technology for rabbit fur characters can be established, and the molecular marker-assisted breeding technology is applied to genetic improvement of the fur weight and quality of rabbits and improvement of rabbit fur value and breeding benefits.

The utility model relates to rabbit hair sampling device technical field, specifically disclose a rabbit hair sampling device for domestic rabbit sampling, including cage, be equipped with the component that drives in the cage, one end of cage is fixedly connected with the limiting frame, the side of limiting frame near the cage is equipped with first through -hole, the top of limiting frame is equipped with second through -hole, the both sides of top of limiting frame are equipped with first slide hole and second slide hole respectively, first slide hole is connected with the first sliding plate of sliding in, second slide hole is connected with the second sliding plate of sliding in, in the utility model, let limiting frame receive domestic rabbit through first through -hole, cooperate slidable first sliding plate, second sliding plate, can close the front and back area of limiting frame quickly, the domestic rabbit is stably positioned, ensure its back and second through -hole accurate correspondence, avoid sampling area deviation. Sampling component completes rabbit hair collection through second through -hole, need not the direct contact of person and domestic rabbit, reduce the cross infection risk, promote sampling efficiency.

The invention is suitable for the technical field of rabbit breeding, and provides a method for breeding a rabbit variety with an improved growth speed. According to the invention, a rabbit variety with an improved growth rate is cultivated by using an SpRY-CBE gene editing technology, a specific sgRNA sequence is designed aiming at a CEP104 gene sequence of a New Zealand rabbit, base mutation is carried out on a C site at the sixth site of the sequence, embryo transplantation is carried out after embryo injection, and a new rabbit variety with an improved growth rate is successfully obtained. According to the method, a new thought of animal genetic breeding is developed, a new rabbit model or strain is created, the growth speed of rabbits is effectively increased, and significant improvement of genetic characters is achieved. The achievement is of great significance for promoting the development of the livestock breeding industry in China and improving the economic benefits of the animal industry, and meanwhile, a new way is provided for improving the economic value of animals.

The application discloses a feed containing astragalus polysaccharide and a preparation method thereof, and belongs to the feed field. The application can effectively alleviate the adverse effects of heat stress on female rabbits by adding astragalus polysaccharide in the basic feed, and can improve the conception rate, the number of live kits per litter and the average weight of live kits of the female rabbits, so that the breeding performance is optimized, the growth of kits is promoted, and the economic benefits of breeding are improved. The feed prepared by the application can effectively improve the serum hormone level of animals and reduce the inflammatory factor level, and effectively improve the diversity of intestinal microorganisms of animals under heat stress, so as to provide a scientific basis for precise feeding management of rabbits in summer, and also provide an important reference for the application of astragalus polysaccharide in the field of stress resistance of livestock and poultry.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to rabbit feed conversion rate and application of the SNP molecular marker, and belongs to the technical field of molecular marker-assisted breeding. The SNP site is located at the position of 10140721bp of a chromosome 5 of a reference genome of the Kangbig meat rabbit, and the polymorphism of the SNP site is G / T. According to the SNP locus and the detection method provided by the invention, an efficient and accurate molecular marker-assisted breeding technology can be established, and when the molecular marker-assisted breeding technology is applied to breeding rabbit feed conversion rate character breeding, the feed conversion efficiency of rabbits can be genetically improved, and the breeding benefit can be improved.

The utility model relates to the technical field of medical teaching instruments, in particular to a rabbit ear edge intravenous injection simulation training device. The domestic rabbit ear edge intravenous injection simulation training device comprises a rabbit ear model, an injector and an indicating circuit, at least one ear edge vein model is arranged in the rabbit ear model, each ear edge vein model comprises an insulating sleeve used for simulating blood vessels and a conductive core used for simulating blood, and the conductive cores are located on the inner rings of the insulating sleeves. An anti-puncture layer is arranged on one side of the rabbit ear model, and a conductive layer is arranged between the anti-puncture layer and the ear edge vein model; the indicating circuit comprises a power source, a first indicator and a second indicator, the first indicator and the second indicator are electrically connected with one electrode of the power source, the first indicator and the second indicator are connected in parallel, the first indicator is electrically connected with the conductive core, and the second indicator is electrically connected with the injector and the other electrode of the power source. By adopting the mode, the problem of liquid leakage in the training process can be avoided, and the safety during intravenous injection simulation training is improved.

The invention belongs to the technical field of biological medicines, and particularly relates to an H4K31 crotonylation modified antigen epitope peptide, a polyclonal antibody and a preparation method and application of the H4K31 crotonylation modified antigen epitope peptide. The invention provides an H4K31 crotonylation modified antigen epitope peptide, the amino acid sequence of the H4K31 crotonylation modified antigen epitope peptide is shown as SEQ ID NO.1 in a sequence table, and the ninth amino acid is subjected to crotonylation modification. According to verification of the invention, the antigen epitope peptide can be used for preparing an effective polyclonal antibody at domestic rabbits, and the obtained antibody can specifically recognize H4K31 crotonylation modification in animal cells and tissues, and can be used for detecting the H4K31 crotonylation level of animal and human samples.