12 results about "European rabbit" patented technology

The utility model relates to the technical field of rabbit cages, and discloses a transfer box for rabbit breeding, which comprises a rabbit cage body, and the rabbit cage body comprises a base and a cage body hinged on the base; a detachable grid plate matched with the base is arranged at the bottom of the cage body, and a screen cover body is hinged to the top of the cage body; a movable water feeder is arranged on the side wall of the cage body; the water feeder comprises a stud rotationally arranged in a hole of the cage body, a nut is arranged at the end, located outside the cage body, of the stud, a water storage bottle is fixedly arranged at the end, located in the cage body, of the stud, the bottom of the water storage bottle is communicated with a pipeline, the end, away from the water storage bottle, of the pipeline is in an inverted cone shape, a rod body is arranged in the pipeline, and a spring is arranged on the rod body. The other end of the spring is provided with a ball which abuts against the water outlet of the pipeline for sealing. Compared with the prior art, the domestic rabbit transfer box has the advantages that excrement of domestic rabbits is convenient to clean, and the stability is good when the transfer box is stacked.

The invention discloses a domestic rabbit whole genome multifunctional chip and application, and belongs to the technical field of molecular biology. The invention relates to a rabbit whole genome 10K liquid phase breeding chip based on a target area genome sequence liquid phase capture accurate positioning sequencing typing technology. The chip comprises 10331 SNP (Single Nucleotide Polymorphism) loci. The chip can be applied to rabbit genetic diversity evaluation, germplasm resource and genetic relationship identification, important economic character whole genome association analysis and whole genome selective breeding, has the advantages of high throughput, high interval coverage rate, high locus detection rate, high flexibility and the like, and provides powerful tool support for rabbit molecular breeding and variety identification.

The utility model belongs to the technical field of instruments for animal experiments, discloses a rabbit ear edge intravenous injection fixing device, and aims to solve the problems that in the daily operation process, due to struggling of a domestic rabbit body and emergency response of rabbit ears, the rabbit body rolls and shakes, an injection needle easily penetrates through the rabbit ears, and fingers of an operator are injured. The technical scheme of the utility model is as follows: the experiment table comprises a working table, a lighting device is fixedly arranged on the top surface of the working table, a base is arranged on the top surface of the working table, vertical plates which are uniformly distributed are fixedly arranged on the top surface of the base, and the vertical plates are fixedly arranged on the top surface of the working table. According to the rabbit ear edge intravenous injection device, a rabbit can be fixed through the front plate, the inserting plate and the fixed plate, rabbit ears and the movable plate can be fixed through the binding device, the movable plate can move along the transverse shaft, the position angle of the rabbit ears placed on the movable plate can be adjusted conveniently, and an operator can conduct rabbit ear edge intravenous injection operation conveniently; the device can be operated by one person and is simple and convenient to use.

The invention is applicable to the technical field of animal model construction, and provides a method for constructing a rabbit growth trait model based on ZDHHC23 gene knockout, and the method comprises the following steps: transcription synthesis of SpG-Cas9 mRNA; the method comprises the following steps: designing and synthesizing a zinc finger structural domain sgRNA of a rabbit ZDHHC23, namely designing an action target sgRNA on a rabbit ZDHHC23 gene aiming at the zinc finger structural domain of the rabbit ZDHHC23; and preparing the ZDHHC23 gene edited rabbit by an embryo microinjection technology. According to the embodiment of the invention, a SpG-Cas9 gene editing technology is utilized, gene knockout is carried out on the zinc finger structural domain of the rabbit ZDHHC23, a new variety of the rabbit with increased weight is obtained, and the method has application value in promoting improvement of genetic characters of the rabbit and improving production performance and economic value of the rabbit.

The invention relates to a molecular marker related to tenderness of rabbit meat and application of the molecular marker, and belongs to the technical field of rabbit molecular marker breeding. The molecular marker is SNP (Single Nucleotide Polymorphism) and is located at 77978399bp of chromosome 11 of a reference genome of a Kangbig meat rabbit, the polymorphism of the molecular marker is C / T, and the tenderness of the rabbit meat of an individual with the genotype of TT or CT at the site is good. According to the SNP site and the detection method provided by the invention, a molecular marker-assisted breeding technology for tenderness of rabbit meat can be established, and the molecular marker-assisted breeding technology is applied to genetic improvement of tenderness of rabbit meat and improvement of rabbit meat quality and economic benefits.

The application discloses a feed containing astragalus polysaccharide and a preparation method thereof, and belongs to the feed field. The application can effectively alleviate the adverse effects of heat stress on female rabbits by adding astragalus polysaccharide in the basic feed, and can improve the conception rate, the number of live kits per litter and the average weight of live kits of the female rabbits, so that the breeding performance is optimized, the growth of kits is promoted, and the economic benefits of breeding are improved. The feed prepared by the application can effectively improve the serum hormone level of animals and reduce the inflammatory factor level, and effectively improve the diversity of intestinal microorganisms of animals under heat stress, so as to provide a scientific basis for precise feeding management of rabbits in summer, and also provide an important reference for the application of astragalus polysaccharide in the field of stress resistance of livestock and poultry.

The utility model relates to the technical field of medical teaching instruments, in particular to a rabbit ear edge intravenous injection simulation training device. The domestic rabbit ear edge intravenous injection simulation training device comprises a rabbit ear model, an injector and an indicating circuit, at least one ear edge vein model is arranged in the rabbit ear model, each ear edge vein model comprises an insulating sleeve used for simulating blood vessels and a conductive core used for simulating blood, and the conductive cores are located on the inner rings of the insulating sleeves. An anti-puncture layer is arranged on one side of the rabbit ear model, and a conductive layer is arranged between the anti-puncture layer and the ear edge vein model; the indicating circuit comprises a power source, a first indicator and a second indicator, the first indicator and the second indicator are electrically connected with one electrode of the power source, the first indicator and the second indicator are connected in parallel, the first indicator is electrically connected with the conductive core, and the second indicator is electrically connected with the injector and the other electrode of the power source. By adopting the mode, the problem of liquid leakage in the training process can be avoided, and the safety during intravenous injection simulation training is improved.

The invention belongs to the technical field of biological medicines, and particularly relates to an H4K31 crotonylation modified antigen epitope peptide, a polyclonal antibody and a preparation method and application of the H4K31 crotonylation modified antigen epitope peptide. The invention provides an H4K31 crotonylation modified antigen epitope peptide, the amino acid sequence of the H4K31 crotonylation modified antigen epitope peptide is shown as SEQ ID NO.1 in a sequence table, and the ninth amino acid is subjected to crotonylation modification. According to verification of the invention, the antigen epitope peptide can be used for preparing an effective polyclonal antibody at domestic rabbits, and the obtained antibody can specifically recognize H4K31 crotonylation modification in animal cells and tissues, and can be used for detecting the H4K31 crotonylation level of animal and human samples.

The invention provides a preparation method of a triple recombinant vaccine, which is mainly used for preparing rabbit hemorrhagic syndrome, rabbit hepatitis E and rabbit pox virus recombinant vaccines. The method comprises the following steps: extracting RHDV VP60 and HEV-ORF2 genes, constructing recombinant plasmids, transfecting the recombinant plasmids into competent cells, screening and identifying the recombinant plasmids, culturing the recombinant plasmids in rabbit kidney cells, amplifying after pressurizing and screening, harvesting recombinant viruses, repeatedly freezing and thawing, and freezing and storing. The purpose of preventing rabbit pox viruses, rabbit hepatitis E and rabbit viral syndromes is achieved by vaccination of rabbits, production is easy, the problem of cold storage does not exist, three viruses are prevented through one injection, meat body pain and local red and swollen caused by multiple injections to animals are avoided, and stress response is reduced. Meanwhile, the rabbit pox is a carrier of the species, so that the interference caused by a non-species carrier is reduced, the stimulation to inoculated animals is small, the immune effect is strong and the like, and the research of the rabbit pox carrier vaccine has wide theoretical significance and practical significance.

The application discloses a kind of Marninif basket fungus colp6-2 protein polyclonal antibody and preparation method, with the amino acid sequence shown in SEQ ID NO.1 as immunogen, first obtain the recombinant protein of the amino acid sequence;Meanwhile, inactivated Marninif basket fungus is used to immunize domestic rabbit, and antiserum is obtained by blood taking;Then the polyclonal antibody of Marninif basket fungus colp6-2 protein is obtained by using recombinant protein to purify antiserum, the polyclonal antibody can both antigen identify Marninif basket fungus;And can recognize recombinant protein antigen, specificity is strong, and detection sensitivity is high.The application also discloses a kit for detecting Marninif basket fungus, and the biotin-labeled Marninif basket fungus colp6-2 protein polyclonal antibody is used to detect Marninif antigen in sample, and the detection specificity is strong, and the sensitivity is high.

This invention discloses a strain of *Pediococcus lactis* GBW9011 and its preparation as a solid microbial preparation for preventing diarrhea in weaned rabbits, belonging to the field of microbial preparations. This invention provides a strain of *Pediococcus lactis* GBW9011, with the preservation number CGMCC No. 34454. This invention utilizes *Pediococcus lactis* GBW9011 to ferment and enrich inorganic zinc ions, preparing zinc-rich lactic acid bacteria powder. This powder is then mixed with a compound enzyme preparation, fructooligosaccharides, and compound vitamins to prepare a solid microbial preparation. This solid microbial preparation can prevent diarrhea in weaned rabbits, exerting a synergistic effect through "inhibiting pathogens, strengthening the barrier, aiding digestion, and resisting stress," significantly reducing the incidence of diarrhea in weaned rabbits, increasing weight gain and survival rates, improving digestive enzyme activity, and improving intestinal flora structure. This has significant implications for animal husbandry, including rabbits.

The utility model relates to the technical field of biomedical experiment instrument, especially relates to a fixing device for visual auricular margin intravenous injection of domestic rabbit. Including the adjusting plate, the support rod with the thread is set to the both sides of the front part of adjusting plate, two support rods sliding type pass through the front end of the placing frame, and still be screw set with the fixed peg of the front end of placing frame on the support rod, the both sides of adjusting plate are hingedly set with the rotating seat, the connecting seat is hingedly set on the rotating seat, the connecting seat is connected with the mounting plate, the mounting plate is provided with the guide rod, a group of interval layout's bandage is slidingly set on the guide rod. The utility model adopts the rotary cooperation design of connecting seat and rotating seat, and the mounting plate angle is flexibly adjustable, so as to adapt to the rabbit ear position of different individuals, and then cooperate with the mounting plate and bandage, can accurately fix rabbit ear, ensure that rabbit ear can be properly unfolded, convenient for accurately finding the venous position, provide great convenience for experiment personnel.