Method for forecasting field kendir rust disease and special device therefor
A technology of apocynum and rust, which is applied in the field of prediction of apocynum rust, and can solve problems such as leaf rot, false alarm, affecting infection and spore production effect, etc.
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[0048] The present invention will be further described below in combination with specific embodiments.
[0049] Carrier production: Cultivate apocynum plants in a sterile environment and take their leaves as capture carriers.
[0050]a. Production of culture medium: Choose commercially available vermiculite with coarser particles and good air permeability, generally choose a particle diameter of 1-3 mm, sterilize the vermiculite in an oven at 120-171 °C for 48-72 h, take out Put into seedling trays.
[0051] b. Germination: Select 80-100 g of apocynum seeds collected in the year, disinfect with 0.1-0.5% sodium hypochlorite solution for 3-5 min, filter with gauze and then rinse with sterile water for 3-5 times. Then put the seeds into a triangular flask, tie the mouth of the flask with parafilm, and put it into a water bath at 40°C for germination for 12-15h. When the seed buds appear white, pour out the water in the triangular flask.
[0052] c. Colonization: the seeds that...
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