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Method for forecasting field kendir rust disease and special device therefor

A technology of apocynum and rust, which is applied in the field of prediction of apocynum rust, and can solve problems such as leaf rot, false alarm, affecting infection and spore production effect, etc.

Active Publication Date: 2014-07-23
XINJIANG ALTAY PREFECTURE GAUBAO KENDER CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the detached leaves inoculated with P. palustris need a certain humidity environment to infect and show symptoms, and the leaves of Apocynum apocynum sampled in the field are easily polluted by other miscellaneous bacteria, causing the leaves to rot rapidly, thus affecting the infection and sporulation effect, and ultimately causing The entire detection is far from reality, and it has also become an important reason for false positives

Method used

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  • Method for forecasting field kendir rust disease and special device therefor
  • Method for forecasting field kendir rust disease and special device therefor
  • Method for forecasting field kendir rust disease and special device therefor

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Embodiment Construction

[0048] The present invention will be further described below in combination with specific embodiments.

[0049] Carrier production: Cultivate apocynum plants in a sterile environment and take their leaves as capture carriers.

[0050]a. Production of culture medium: Choose commercially available vermiculite with coarser particles and good air permeability, generally choose a particle diameter of 1-3 mm, sterilize the vermiculite in an oven at 120-171 °C for 48-72 h, take out Put into seedling trays.

[0051] b. Germination: Select 80-100 g of apocynum seeds collected in the year, disinfect with 0.1-0.5% sodium hypochlorite solution for 3-5 min, filter with gauze and then rinse with sterile water for 3-5 times. Then put the seeds into a triangular flask, tie the mouth of the flask with parafilm, and put it into a water bath at 40°C for germination for 12-15h. When the seed buds appear white, pour out the water in the triangular flask.

[0052] c. Colonization: the seeds that...

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Abstract

The invention discloses a method for forecasting a kendir rust disease caused by kendir Melampsora and a special device therefor. The method mainly comprises the following steps: manufacturing of a carrier, preparation of a sampling device, sampling and trapping of spores, isolated culture and pathogenicity calculation. The occurrence of the Melampsora of the field-planted kendir can be accurately forecast and predicted, so that a control plan can be effectively made. Compared with the prior art, the invention can effectively determine the quantity of pathogenic bacteria having infectivity while the quantity of pathogenic bacteria can be determined, and especially can solve the problem that isolated kendir leaves which have been sampled in an aeroscope can be easily polluted and rotten in the culture process. Thus, the invention is suitable for forecasting and predicting the Melampsora of the field commercially-planted kendir.

Description

technical field [0001] The invention belongs to the category of plant disease prevention, and mainly relates to the technology of detecting and forecasting the important disease rust of apocynum apocynum, in particular a method for predicting the rust of apocynum apocynum caused by Pseudomonas spp. and a special device for the method . Background technique [0002] With the growing area and output of Apocynum apocynum, grid rust has become the main disease in Apocynum apocynum plantation, which has an important impact on the growth and quality of apocynum apocynum, especially brings great economic losses to production, and seriously hinders the development of apocynum apocynum. The development of the hemp industry. [0003] The ureterospores of Apocynum palustridium are the source of infection for the occurrence and development of Prussia apocynum. Therefore, accurate determination of the number of uredia spores in the air in the field, especially the pathogenicity, can pro...

Claims

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Application Information

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IPC IPC(8): C12Q1/06C12M1/26C12M1/22C12R1/645
Inventor 高鹏刘起棠南志标段廷玉黄景凤孟繁杰张峰
Owner XINJIANG ALTAY PREFECTURE GAUBAO KENDER CO LTD
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