137results about How to "Suitable for automation" patented technology

Methods to detect, characterize, and quantitate biological samples after administration of antibody conjugates, antibody-drug conjugates of Formula I, antibodies, and fragments and metabolites thereof, by immunoaffinity bead separation, chromatography, and mass spectrometry are disclosed.Ab-(L-D)p  IwhereinAb is an antibody;D is a drug moiety;L is a linker covalently attached to Ab, and covalently attached to D; andp is 1, 2, 3, 4, 5, 6, 7, or 8;

An improved method of amplifying nucleic acids comprising the use of four discrete temperature steps in a thermocyclic amplification reaction, as well as, a method of detecting large nucleic acid insertions or deletions such as those that occur from gene duplication or deletion.

The present invention provides novel methods, compositions, and kits for the production of amplification-ready, sequence-specific, target region-specific, and strand-specific regions of interest directly from samples containing complex DNA. The methods, composition, and kits provided herein are useful for selective target generation, genome partitioning, or user-selected enrichment of desired regions of interest. The invention described herein will enable multiplexing for genome-wide analysis with increased efficiency and is amenable to automation.

The present invention relates to multi-phase protein separation methods capable of resolving and characterizing large numbers of cellular proteins, including methods for efficiently facilitating the transfer of protein samples between separation phases. In particular, the present invention provides an automated system for the separation, identification, and characterization of protein samples. The present invention thus provides improved methods for the analysis of samples containing large numbers of proteins.

The invention provides an apparatus and process for detecting the degree of confluence of animal cells being cultured in a well plate. A well plate is arranged in an imaging station and illuminated with a ring of LEDs, or other optical source, from below at an oblique angle. An image of the well is captured with a CCD camera or other detector from above or below, such that the well image is taken in a dark field configuration where light from the optical source, if not scattered, does not contribute to the well image. By the simple solution of illuminating wells of a well plate from below at an oblique angle, it has been found that many animal cell types can be imaged with sufficient contrast to allow cell identification and consequent cell area computation using image processing techniques, thereby allowing confluence to be determined of animal cells being cultured in well plates. This avoids the need for more complex optical imaging techniques, such as phase contrast microscopy.

The invention provides an improved method for detecting cancer or a precancerous condition in a sample using an oxidation agent, such as galactose oxidase, and an aldehyde detection agent, such as Schiff's reagent that does not require the sample to be immobilized onto a solid support. The invention also provides kits comprising the components necessary for carrying out the methods of the invention.

An electrical accumulator having a case in the form of a button cell which is closed such that it is gastight, including a case cover and a case cup with an interposed electrically insulating seal, which contain an electrode set including positive and negative electrodes as well as interposed separators, with at least two electrodes of one polarity being mechanically. and electrically connected at at least two points on their circumference in such a manner that a mechanically robust electrode packet results. The invention furthermore relates to a method for producing such a rechargeable battery.

A method for comparing a data set to a baseline value comprising: providing the data set to be analyzed; locating potentially bad data points in at least a portion of the data set using an odd-man out recursive technique; preparing a baseline set by discarding the potentially bad data points from the at least a portion of the data set; and calculating a baseline value from the baseline set.

The present invention describes a method for identifying one or more of a plurality of sequences differing by one or more single base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences. The method includes a ligation phase, a capture phase, and a detection phase. The ligation phase utilizes a ligation detection reaction between one oligonucleotide probe, which has a target sequence-specific portion and an addressable array-specific portion, and a second oligonucleotide probe, having a target sequence-specific portion and a detectable label. After the ligation phase, the capture phase is carried out by hybridizing the ligated oligonucleotide probes to a solid support with an array of immobilized capture oligonucleotides at least some of which are complementary to the addressable array-specific portion. Following completion of the capture phase, a detection phase is carried out to detect the labels of ligated oligonucleotide probes hybridized to the solid support. The ligation phase can be preceded by an amplification process. The present invention also relates to a kit for practicing this method, a method of forming arrays on solid supports, and the supports themselves.

Devices and methods are described for homogenization, processing, detection, and analysis of biological samples such as insects, fungi, bacteria, and plant and animal tissues. Multiple chambers in these devices permit different processing functions to be carried out at each stage, such that the resulting homogenized product can be further processed, purified, analyzed, and / or biomolecules such as metabolites, proteins and nucleic acids, or pharmaceutical products can be detected. The device can be used in a hydrostatic pressure apparatus, in which different activities, i.e. incubations, addition or renewal of reagent, and generation and detection of signal can be carried out in the appropriate chamber. The method improves the preservation of biomolecules from chemical and enzymatic degradation relative to conventional means. Additionally, this method enables automated sample preparation and analytical processes.

The present invention relates to nucleotide sequences, including expressed sequence tags (ESTs), oligonucleotide probes, polypeptides, vectors and host cells expressing, and immunoadhesions and antibodies to mammalian GFRα3, a novel α-subunit receptor of the GDNF (i.e. GFR) receptor family. It further relates to an assay for measuring activation of an α-subunit receptor by detecting tyrosine kinase receptor activation (i.e., autophosphorylation) or other activities related to ligand-induced α-subunit receptor homo-dimerization or homo-oligomerization.

A method of isolating target nucleic acid molecules from a solution comprising a mixture of different size nucleic acid molecules, in the presence or absence of other biomolecules, by selectively facilitating the adsorption of a particular species of nucleic acid molecule to the functional group-coated surface of magnetically responsive paramagnetic microparticles is disclosed. Separation is accomplished by manipulating the ionic strength and polyalkylene glycol concentration of the solution to selectively precipitate, and reversibly adsorb, the target species of nucleic acid molecule, characterized by a particular molecular size, to paramagnetic microparticles, the surfaces of which act as a bioaffinity adsorbent for the nucleic acids. The target nucleic acid is isolated from the starting mixture based on molecular size and through the removal of magnetic beads to which the target nucleic acid molecules have been adsorbed. The disclosed method provides a simple, robust and readily automatable means of nucleic acid isolation and purification which produces high quality nucleic acid molecules suitable for: capillary electrophoresis, nucleotide sequencing, reverse transcription cloning the transfection, transduction or microinjection of mammalian cells, gene therapy protocols, the in vitro synthesis of RNA probes, cDNA library construction and PCR amplification.

The object of the invention is to provide a method of calibrating an optical FMCW backscattering measurement system that improves the precision of the measurement. The problem is solved by a method comprising the steps of A. Converting said received sensor signal to a complex received electrical signal as a function of said modulation frequency fm, said complex received electrical signal being represented by a magnitude part and a phase angle part as a function of said modulation frequency fm; B. Performing a transformation of said received electrical signal to provide a backscattering signal as a function of location between said first and second ends of said sensor and beyond said second end; C. From said backscattering signal as a function of location determining characteristics of a curve representative of said backscattering signal beyond said second end; D. Correcting said magnitude part of said received electrical signal and said phase angle part of said received electrical signal in a predetermined dependence of said curve; and E. Repeating step B) on the basis of the corrected received electrical signal.

A method and device to assist in the determination of protein domain boundaries is described. In particular the device is designed to provide a high throughput of proteolytic digestion of proteins to identify domains and their boundaries, for use in protein structure determination, in a manner that is amenable to automation. Proteases are immobilized in a convenient format such as a microtitre plate and preferably arranged in a matrix thereby allowing for simultaneous degradation of a protein by a number of proteases at a number of concentrations.

The present invention relates to novel complex peptidomimetic products comprising multiple homogeneous or heterogeneous pendant groups that are site-specifically positioned along a linear oligomer or polymer scaffold and methods of making thereof. More specifically, the invention relates to N-substituted glycine peptoid oligomers or peptoids and their use as substrates for azide-alkyne [3+2]-cycloaddition conjugation reactions and subsequent additional rounds of oligomerization and cycloaddition. The methods of the invention may also be used to generate peptoid-peptide hybrid or peptide products comprising multiple homogeneous or heterogeneous pendant groups, which are positioned precisely along the linear oligomer or polymer scaffold.

The present invention relates to nucleotide sequences, including expressed sequence tags (ESTs), oligonucleotide probes, polypeptides, vectors and host cells expressing, and immunoadhesions and antibodies to mammalian GFRα3, a novel α-subunit receptor of the GDNF (i.e. GFR) receptor family. It further relates to an assay for measuring activation of an α-subunit receptor by detecting tyrosine kinase receptor activation (i.e., autophosphorylation) or other activities related to ligand-induced α-subunit receptor homo-dimerization or homo-oligomerization.

The embodiments of the invention described herein relate to systems and methods for the vitrification of biological samples. Vitrification is achieved by generating nanodroplets of a solution comprising the biological sample with a means that can be automated and adapted to high-throughput applications.

A quantity (or dispersion value) of a distribution of edge position due to random noise is expected to be reduced statistically to 1 / N when N edge position data items are averaged. Using this property, the single page image is averaged in a vertical direction with various values of parameter S, and then the edge roughness index is calculated. The S-dependence of the edge roughness index is analyzed and a term of a dispersion value directly proportional to 1 / S is determined as being due to noise.

The present invention relates to multi-phase protein separation methods capable of resolving and characterizing large numbers of cellular proteins, including methods for efficiently facilitating the transfer of protein samples between separation phases. In particular, the present invention provides systems and methods for the generation of multi-dimensional protein maps. The present invention thus provides improved methods for the analysis of samples containing large numbers of proteins.

The current invention relates to the field of detection and identification of clinically important fungi. More particularely, the present invention relates to species specific probes originating from the Internal Transcribed Spacer (ITS) region of rDNA for the detection of fungal species such as Candida albicans, Candida parapsilosis, Candida tropicalis, Candida kefyr, Candida krusei, Candida glabrata, Candida dubliniensis, Aspergillus flavus, Aspergillus versicolor, Aspergillus nidulans, Aspergillus fumigatus, Cryptococcus neoformans and Pneumocystis carinii in clinical samples, and methods using said probes.

A method for estimation of a probe shape, in a scanning electron microscope provided with an aberration corrector, and the method is designed so as to obtain a probe image, by inputting to a computer an image taken in a just-focused state and an image taken in a de-focused state, as an image data; preparing a correlation window by automatically determining a size of a correlation window image, based on an input data size and an output data size; executing cross-correlation calculation between the correlation window and a reference area; and repeating this calculation while shifting the reference area, so as to obtain a cross-correlation matrix, in order to stably obtain the probe image, without receiving effects of use conditions or noises.

The invention relates to a novel packing case and the manufacturing process thereof, which is characterized in that the novel packing case comprises an upper casing body and a lower casing body which are oppositely covered, and an elastic tensioning diaphragm which can elastically clip objects when the casing bodies are oppositely covered is respectively adhered to the upper casing body and the lower casing body by adopting UV adhesive. The novel packing case has the advantages that the structure is simple, the design is reasonable, is in favor of simplifying the production processes, reducing the production cost and re-utilization. Due to the advantages of the UV adhesive, the elastic tensioning diaphragm which is adhered by adopting the adhesive has the significant advantages of high bonding strength, high speed and high degree of automation, etc.

The invention provides a preparation method of benzaldehyde and its derivatives. The preparation method comprises adding a catalyst and toluene or toluene homolog into a reaction system in the presence of an oxidizing agent, and carrying out a one-step reaction process to produce benzaldehyde or its derivatives, wherein the catalyst comprises a metal catalyst, an auxiliary catalyst I and an auxiliary catalyst II, the metal catalyst is a transition metal catalyst, the auxiliary catalyst I is a basic nitrogen-containing ligand, and the auxiliary catalyst II is a basic compound. The preparation method can prepare benzaldehyde and its derivatives through one-step direct oxidation of toluene, utilizes cheap raw materials having wide sources, has a fast reaction speed and high production intensity, is suitable for automatic and continuous production, does not use chlorine, is suitable for industrial production and has a good application prospect.

The invention provides a permanent magnet with a corrosion resistant coating. The permanent magnet comprises a permanent magnetic material base body 1, an organic matter bottom layer 2, an organic matter transition layer 3 and a nanometer silver layer 4, and also comprises a powder nanometer spraying method for the permanent magnet with the corrosion resistant coating. As the metal base body is isolated from the contacted outside corrosion media through the inner and outer alloy layers, the metal base body is difficult to corrode and has good humidity and heat resistance property, and then the service life of the permanent magnet is prolonged. As the coating is thin, the influence on the magnet is small, and the magnet shielding does not exist. The product investment cost is reduced, and the safety production is ensured.

The present invention relates to novel complex peptidomimetic products comprising multiple homogeneous or heterogeneous pendant groups that are site-specifically positioned along a linear oligomer or polymer scaffold and methods of making thereof. More specifically, the invention relates to N-substituted glycine peptoid oligomers or peptoids and their use as substrates for azide-alkyne [3+2]-cycloaddition conjugation reactions and subsequent additional rounds of oligomerization and cycloaddition. The methods of the invention may also be used to generate peptoid-peptide hybrid or peptide products comprising multiple homogeneous or heterogeneous pendant groups, which are positioned precisely along the linear oligomer or polymer scaffold.

Provided are methods for selectively identifying and isolating nucleic acids in a population of nucleic acid molecules.

A method for compensating output angular speed information of optical fiber gyroscope in temperature-control free and closed loop light source type includes applying regression analysis means to obtain adapting relation of central wavelength of outgoing light from temperature-control free light source to temperature then compensating optical gyroscope output angular speed information drift caused by temperature variation in utilizing optical gyroscope to output relevant relation value of central wavelength at outgoing light on temperature-control free light source.

A scanning charged particle microscope which facilitates adjustment, has a deep focal depth, and is provided with an aberration correction means. The state of aberration correction is judged from a SEM image by using a stop having plural openings and the judgment result is fed back to the adjustment of the aberration correction means. A stop of a nearly orbicular zone shape is used in combination with the aberration correction means.

Methods to detect, characterize, and quantitate biological samples after administration of antibody conjugates, antibody-drug conjugates of Formula I, antibodies, and fragments and metabolites thereof, by immunoaffinity bead separation, chromatography, and mass spectrometry are disclosed;Ab-(L-D)p  IwhereinAb is an antibody;D is a drug moiety;L is a linker covalently attached to Ab, and covalently attached to D; andp is 1, 2, 3, 4, 5, 6, 7, or 8.