10 results about "Genome wide analysis" patented technology

The present invention relates to the field of spatial epigenomic profiling. It concerns DNA arrays loaded with barcoded polynucleotides harboring a mosaic (MOS) sequence, methods and uses thereof enabling spatial epigenomic profiling.

The application discloses a kind of based on machine learning's genome analysis parallel optimization method and system, the method of the present application includes the input BAM file is cut into multiple same size file blocks;For each file block, extract file block characteristics, and input the file block characteristics into the pre-trained machine learning model to obtain the predicted running time of the file block;The predicted running time is in the front part file block is divided into smaller file block;File block is input into HaplotypeCaller in parallel to carry out variation detection;The variation detection result generated by HaplotypeCaller for each file block is merged and output.The present application is aimed at solving the problem of serious calculation tilt and low resource utilization caused by unclear calculation complexity before HaplotypeCaller runs, improving the efficiency of genome analysis, shortening the variation detection duration.

The disclosure relates to comprehensive genomic profiling (CGP) and to consensus-based classification techniques for determining genetically inferred ancestry from CGP of tumor DNA. Aspects are directed towards accessing reference and subject sequencing files and identifying genomic variants using a hybrid variant tool. The reference variant file is consolidated into a datastore formatted file that is queried to perform joint variant calling to generate a final reference variant file. The final reference variant file and the subject variant file are merged. On the merged variant file, principal component (PC) analysis is performed, and the PCs are used by a first and second classification process to generate a first and second ancestry call. The merged variant file is input into a third classification process to generate a third ancestry call. A consensus genetically inferred ancestry (GIA) call is predicted based on the first, the second, and the third ancestry calls.

[Problem] To provide technology for assisting in making better use of personnel by utilizing the results of personality tests on other personnel. [Solution] An information processing device 1 is capable of performing personality estimation in various models using answer results from a questionnaire, image data obtained by imaging the face, image data obtained by imaging the upper body or the entire body, and genome analysis results as personal information for personality estimation. Regarding the personality of an arbitrary person among target people for whom the personality estimation was performed, a distribution identification unit 1C and a position calculation unit 1D postulate target people belonging to a group of target people which is postulated for said arbitrary person, for example an organization or a divided unit within an organization (e.g. a department), and calculate a relative position. Thereby, the personality of the arbitrary person can be understood as a relative position within the postulated group.

This invention provides a *Roseolariomoraxella salina* strain for saline-alkali soil improvement, with accession number CGMCC No. 36067. The *Roseolariomoraxella salina* strain provided by this invention exhibits significant comprehensive application advantages. This strain demonstrates excellent survival and growth capabilities under saline-alkali stress, effectively reducing salinity and alkalinity in saline-alkali soils and thus improving the saline-alkali environment. Whole-genome analysis shows that this strain carries multiple functional genes related to osmotic regulation and ion transport, providing a molecular basis for its salinity-reducing ability. Adding citric acid or betaine can further enhance its salinity-reducing performance, and its biomass is significantly increased after optimization of fermentation conditions. This strain shows promising application prospects in saline-alkali soil improvement and saline-alkali aquaculture environment management.

This invention belongs to the field of gene technology and discloses a bacterial strain B11 antagonistic to soft rot disease of Amorphophallus muelleri. Isolated from the rhizosphere soil of Amorphophallus muelleri, it was identified as the golden subspecies of *Pseudomonas aeruginosa*. This strain effectively inhibits the growth of the soft rot pathogen Pcc, reducing the incidence and disease index of soft rot in Amorphophallus muelleri. It also possesses the ability to dissolve organophosphates and produce siderophores, and exhibits broad-spectrum antagonistic activity against various fungal diseases of Amorphophallus muelleri, including white mold, leaf spot, and dry rot. Whole-genome analysis shows that the B11 genome is 6.77 Mb in size, with a GC content of 62.81%, encoding 5,964 genes, including gene clusters related to antibacterial activity such as phenazine, nitropyrrolizin, and siderophores. This strain has good development and application potential in the control of soft rot in Amorphophallus muelleri.

A genomic analysis system that performs analysis without external leakage of genomic data may include memory and a processor for storing instructions. When executed by the processor, the instructions can control the system to store raw genomic data in client-side storage, generate de-identified data from which personal information has been removed from the raw genomic data, transmit the de-identified data to an external server to request genetic analysis, receive analysis results from the external server, and generate a final analysis result in a client environment based on the received analysis results.

In an example method, a chemical cross-linker is introduced to cultured cells, so that at least DNA within the cultured cells is cross-linked and cells having fixed chromatin are formed. The cells having fixed chromatin are exposed to cell lysis to form a crude lysate. The crude lysate is introduced into a flow cell including: one of: i) first and second transposome complex dimers immobilized on a surface of the flow cell, the first transposome complex dimers including a first amplification domain and the second transposome complex dimers including a second amplification domain; or ii) a single transposome complex dimer immobilized on the surface, each of the single transposome complex dimers including two forked transposome complexes; and a primer set immobilized on the surface. Tagmentation of the cross-linked DNA in the crude lysate is initiated.

Provided is a nucleic acid molecule comprising an artificial nucleic acid sequence and / or a complementary sequence thereof, or a partial fragment sequence thereof, the artificial nucleic acid sequence consisting of: (1) one copy each of artificial genes encoding the following non-naturally occurring sequences (a) to (p); (2) artificial intergenic sequences for linking the artificial genes, each independently consisting of a non-naturally occurring random sequence of 10 to 60 nucleotides in length; and (3) a front spacer sequence and an end spacer sequence, each independently consisting of a non-naturally occurring random sequence of 200 to 400 nucleotides in length.

This invention belongs to the field of gene technology and discloses a bacterial strain antagonistic to konjac soft rot and its application. The *Pseudomonas pallens* strain B07 of this invention exhibits significant antagonistic activity against the pathogen causing konjac soft rot, with an inhibition zone diameter of 1.05±0.16 cm. In pot experiments, it reduced the incidence of konjac soft rot by 33.34% and the disease index by 22.22%. Simultaneously, this strain possesses plant growth-promoting characteristics such as phosphorus solubilization, siderophore production, and nitrogen fixation. It also shows broad-spectrum antagonistic activity against eight plant pathogenic fungi, including konjac white mold and *Amorphophallus bulbifer* leaf spot, with inhibition rates ranging from 44.87% to 60.03%. Whole-genome analysis shows that its genome contains 14 secondary metabolite biosynthesis gene clusters, providing the molecular basis for its antibacterial activity. This strain can be applied directly or prepared as a biocontrol agent for the prevention and control of konjac soft rot. It is environmentally friendly, residue-free, and possesses both disease prevention and growth-promoting effects, making it of significant industrial value in the green control of konjac diseases.