Method and system for detecting microorganisms and drug-resistant genes in sample

Abstract

The invention discloses a method for detecting microorganisms in a sample, which belongs to the technical field of metagenome analysis and comprises the following steps: S1, acquiring metagenome sequencing data of the sample; and S2, performing species analysis on the metagenome sequencing data. The method further comprises a step of carrying out drug resistance gene analysis on the metagene sequencing data of the sample. The invention also discloses a system for detecting microorganisms and drug-resistant genes in a sample. On the basis of a comprehensive and accurate database and an intelligent analysis and screening algorithm, pathogenic microorganisms and drug-resistant genes are effectively identified by utilizing a metagenome sequencing method, false positive is effectively reduced,bacteria suspected to correspond to the detected drug-resistant genes can be prompted, and better technical support is provided for accurate diagnosis and treatment of infection; in addition, according to the method and the system, potential new pathogenic microorganisms can be accurately analyzed, and technical support is provided for early warning of new infectious diseases.

Description

technical field [0001] The invention belongs to the technical field of metagenomic analysis, and in particular relates to a method and system for detecting microorganisms and drug resistance genes in a sample. Background technique [0002] Infectious diseases caused by pathogenic microorganisms, especially severe pneumonia, sepsis and encephalitis and meningitis, are difficult to identify the pathogens of difficult and critical infectious diseases, which has always been a key problem faced by infectious disease clinicians. Therefore, accurate The identification of pathogenic microorganisms is a crucial step in precision infection treatment. [0003] Traditional pathogenic microorganism detection techniques are mainly divided into two categories: gene cell culture methods and methods based on specific primers or antibodies (molecular detection) such as PCR detection, multiplex PCR detection, and antigen-antibody hybridization reactions. At present, the routine clinical patho...

AI Technical Summary

Problems solved by technology

[0005] However, the current methods of using metagenomic sequencing to detect microorganisms in samples have long data analysis time, low accuracy of microbial comparison, ineffective filtering of background and contaminating microorganisms, inability to effectively distinguish back-colonized or infected microorganisms, ineffective Insufficient identification of multi-drug resistant bacteria and mixed infections, failure to effectively establish associations between detected drug resistance genes and microorganisms, failure to effectively identify new pathogens, etc.

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