Method for identifying and analyzing ancient DNA sample

A sample, specific area technology, applied in the field of identification and analysis of ancient DNA samples, can solve problems to be improved, and achieve the effect of accurate and reliable comparison results, accurate information, and high reliability

Inactive Publication Date: 2019-03-01
SHENZHEN HUADA GENE INST
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Therefore, current methods for identifying and analyzing ancient DNA samples still need to be improved

Method used

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  • Method for identifying and analyzing ancient DNA sample
  • Method for identifying and analyzing ancient DNA sample
  • Method for identifying and analyzing ancient DNA sample

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Embodiment 1

[0095] According to the method of the present invention shown in the above-mentioned "general method", the ancient DNA sample to be tested is subjected to standardized information analysis, specifically as follows:

[0096] Among them, there are 2 ancient DNA samples to be tested: 1 ancient human bone sample and 1 ancient human hair sample. The two paleontological samples were both provided by the Institute of Vertebrate Paleontology and Paleoanthropology, Chinese Academy of Sciences, and were unearthed about 3,000-8,000 years ago. One of them was a human bone sample (Human_Bone), and the other was a human hair sample (Human_Hair ) (see Table 1).

[0097] The specific process is as follows:

[0098] 1. Acquisition of Illumina next-generation sequencing data

[0099] The present invention is based on Illumina next-generation sequencing data, and the DNA extraction and database construction methods of 2 paleontological samples are detailed in:

[0100] [1]N.Rohland, M.Hofreit...

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Abstract

The invention discloses a method for identifying and analyzing an ancient DNA sample, which comprises a method for acquiring DNA information of a to-be-tested DNA sample, wherein the method for acquiring the DNA information comprises the following steps: building a database for the to-be-tested DNA sample and performing sequencing on the to-be-tested DNA sample, so as to acquire sequencing data; filtering the sequencing data; making comparison on the filtered sequencing data, so as to obtain a comparison result, wherein the comparison result contains the DNA information of the to-be-tested DNAsample, and the comparison allows mispairing of at most four basic groups. With adoption of the method for identifying and analyzing the ancient DNA sample, the DNA information of the ancient DNA sample can be effectively acquired based on the building of the database and the sequencing of the to-be-tested ancient DNA sample; in addition, the information is accurate, is high in reliability and can be used for effectively analyzing genomes of the to-be-tested ancient DNA, such as detection of variation, identification of the ancient DNA, sex determination and assessment on modern DNA contamination rate.

Description

technical field [0001] The invention relates to the technical field of biological sequencing, in particular to a method for identifying and analyzing ancient DNA samples. Background technique [0002] Paleontological samples are crucial to the study of the evolutionary history of modern biological populations. The research results of ancient human genomes have made people realize that the genetic composition of modern humans is not only composed of African ancestors, but also combined with ancient Neanderthals after they left Africa. The genetic communication with the ancient Denisorians has overturned people's understanding of the evolutionary history of modern humans. At the same time, the study of paleontological genomes also plays an irreplaceable role in the study of modern biological populations, especially human natural selection and diseases. The plateau adaptation genes of Tibetans have been proved to be from the genomes of ancient Denisors. penetration. As a gene...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6869
CPCC12Q1/6869
Inventor 郭小森兰天明蒋慧
Owner SHENZHEN HUADA GENE INST
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