The application discloses a primer probe combination, a kit and application for detecting pathogenic microorganisms in a
genital tract, wherein the primer probe combination comprises primer probe sequences shown in SEQ ID NO. 1-SEQ ID NO. 96, the pathogenic microorganisms in the
genital tract include 27 pathogenic microorganisms which can cause diseases in the
genital tract, such as Ureaplasma parvum,
Ureaplasma urealyticum,
Mycoplasma hominis,
Mycoplasma genitalium,
Candida albicans,
Candida glabrata,
Candida tropicalis, Candida auris,
Candida krusei,
Candida dubliniensis and the like, and four common vaginal lactobacilli, such as
Lactobacillus crispatus,
Lactobacillus jensenii,
Lactobacillus gasseri and Lactobacillus iners, and further include a human
internal standard. Based on the
fluorescence quantitative PCR technology, the 27 pathogenic microorganisms which can cause diseases in the genital tract and the four common vaginal lactobacilli can be simultaneously detected in an in-vitro sample, the main pathogenic microorganisms of the genital tract infection can be covered, the evaluation of the vaginal
ecological environment can be satisfied, the detection time is shortened to about 40 minutes, and the specificity and the sensitivity are good.