Combined detection kit for vaginitis
A technology of combined detection and detection of reagents, which is applied in the direction of measuring devices, material analysis by observing the influence of chemical indicators, instruments, etc., can solve the problems of cumbersome procedures, long detection time, and large influence of human subjective factors, and achieve improvement Reliability and accuracy, high sensitivity and specificity, and the effect of avoiding BV missed detection
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example 1
[0027] The preparation of example 1 combined detection kit of the present invention:
[0028] The six holes of the six-way detection reagent card are respectively equipped with whatman glass microfiber filter paper CF2 with a pore size of 4.5mm as the pad carrier for hydrogen peroxide concentration detection reagent pads, leukocyte esterase detection reagent pads, and sialidase detection reagent pads , Proline aminopeptidase detection reagent pad, acetylglucosaminidase detection reagent pad, pH test paper.
[0029] Preparation of the hydrogen peroxide reagent pad: soak the above-mentioned pad carrier in the following soaking solution (hydrogen peroxide concentration detection reagent), which contains 2,4,6-tribromo-3-hydroxybenzoic acid (TBHBA) 0.6 g / L, peroxidase 150KU / L, tetraaminoantipyroline (4-AAP) 0.8g / L, dry after soaking.
[0030] Preparation of leukocyte esterase detection reagent pad: soak the pad carrier in the following soaking solution (leukocyte esterase detecti...
Embodiment 3
[0048] The steps of carrying out the combined determination of bacterial vaginosis with the test kit of the present invention are as follows:
[0049] (1) Take the secretion from the posterior fornix of the vagina with a cotton swab and add 500 μl of diluent to dilute the secretion;
[0050] (2) Respectively equipped with hydrogen peroxide concentration reagent pad, leukocyte esterase assay reagent pad, sialidase assay reagent pad, proline aminopeptidase assay reagent pad, acetylglucosaminidase assay reagent pad, pH test paper Add a drop of diluted vaginal fluid to each well of the blind hole, about 35 μl per drop;
[0051] (3) Add 1 drop of chromogenic solution to the well equipped with the sialidase activity reagent pad;
[0052] (4) Leave the dry chemical reaction device at room temperature for 30 minutes or in a 37°C water bath for 15 minutes;
[0053] (5) Interpretation results:
[0054] Interpret the results according to the color card provided in the kit:
[0055] H...
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