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Combined detection kit for vaginitis

A technology of combined detection and detection of reagents, which is applied in the direction of measuring devices, material analysis by observing the influence of chemical indicators, instruments, etc., can solve the problems of cumbersome procedures, long detection time, and large influence of human subjective factors, and achieve improvement Reliability and accuracy, high sensitivity and specificity, and the effect of avoiding BV missed detection

Inactive Publication Date: 2011-11-23
泰普生物科学(中国)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among these methods, the hanging drop method is the easiest way to check Trichomonas vaginalis, but it is still a relatively cumbersome method to make a smear
[0012] At present, there is no product that can detect the above three types of vaginitis at the same time. Each type of bacteria needs to be tested separately, and microscopic examination is often used. Long, cumbersome procedures, inconvenient
The bacterial vaginosis joint detection kit currently on the market is only used for the detection of bacterial vaginosis and microecological evaluation, and cannot jointly detect candida vaginitis and trichomonas vaginitis, and is not a real joint detection product for vaginitis

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0027] The preparation of example 1 combined detection kit of the present invention:

[0028] The six holes of the six-way detection reagent card are respectively equipped with whatman glass microfiber filter paper CF2 with a pore size of 4.5mm as the pad carrier for hydrogen peroxide concentration detection reagent pads, leukocyte esterase detection reagent pads, and sialidase detection reagent pads , Proline aminopeptidase detection reagent pad, acetylglucosaminidase detection reagent pad, pH test paper.

[0029] Preparation of the hydrogen peroxide reagent pad: soak the above-mentioned pad carrier in the following soaking solution (hydrogen peroxide concentration detection reagent), which contains 2,4,6-tribromo-3-hydroxybenzoic acid (TBHBA) 0.6 g / L, peroxidase 150KU / L, tetraaminoantipyroline (4-AAP) 0.8g / L, dry after soaking.

[0030] Preparation of leukocyte esterase detection reagent pad: soak the pad carrier in the following soaking solution (leukocyte esterase detecti...

Embodiment 3

[0048] The steps of carrying out the combined determination of bacterial vaginosis with the test kit of the present invention are as follows:

[0049] (1) Take the secretion from the posterior fornix of the vagina with a cotton swab and add 500 μl of diluent to dilute the secretion;

[0050] (2) Respectively equipped with hydrogen peroxide concentration reagent pad, leukocyte esterase assay reagent pad, sialidase assay reagent pad, proline aminopeptidase assay reagent pad, acetylglucosaminidase assay reagent pad, pH test paper Add a drop of diluted vaginal fluid to each well of the blind hole, about 35 μl per drop;

[0051] (3) Add 1 drop of chromogenic solution to the well equipped with the sialidase activity reagent pad;

[0052] (4) Leave the dry chemical reaction device at room temperature for 30 minutes or in a 37°C water bath for 15 minutes;

[0053] (5) Interpretation results:

[0054] Interpret the results according to the color card provided in the kit:

[0055] H...

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PUM

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Abstract

The invention aims to provide a combined detection kit for vaginitis, capable of evaluating vaginal micro-ecological environment, vaginal cleanliness, and pathogens of bacterial vaginosis, Candida vaginitis, trichomonas vaginitis of the tested person simultaneously. Ihe following technical scheme adopted by the invention, a combined detection kit for vaginitis is provided and comprises a reaction device, diluent and chromogenic liquid, the reaction device is a hole type box-shaped reaction device with six reacting blind holes, in which a hydrogen peroxide concentration detection reagent pad, a leukocyte esterase detection reagent pad, a neuraminidase detection reagent pad, a proline aminopeptidase detection reagent pad, an acetyl glucosaminidase detection reagent pad and pH detection test paper are arranged respectively. Through the combined detection, the vaginitis caused by most of the BV (Bacterial Vaginosis) pathogens, the Candida albicans and the trichomonas can be detected, BV missing detection caused by the simple detection for one kind of enzyme activity is avoided largely, and the detection reliability and accuracy are increased.

Description

technical field [0001] The invention relates to a combined detection kit for vaginitis, which is especially used for auxiliary diagnosis of three female vaginal diseases: bacterial vaginosis, candida vaginitis and trichomonas vaginitis. Background technique [0002] Bacterial vaginosis (Bacterial Vaginosis, BV) used to be called non-specific bacterial vaginosis. In 1955, Gardner and Dukes first isolated Haemophilus vaginalis from patients with non-specific bacterial vaginosis, and thus called the disease Haemophilus vaginalis bacterial vaginosis. In the 1980s, it was found that this bacterium was different from other Haemophilus bacilli, and it was named Gardnerella vaginalis, and the disease was renamed Gardnerella bacterial vaginosis. In 1983, the most concise name-bacterial vaginosis was selected at the Stockholm International Conference, on the grounds that its pathogen is not only Gardnerella vaginalis, but also other anaerobic bacteria; Rare white blood cells in the ...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N21/78G01N21/80
Inventor 练惠辉胡守旺姚铭锋谢佐福周晓强阳卫超陈宁
Owner 泰普生物科学(中国)有限公司
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