The invention discloses a method for detecting a fingerprint spectrum of cyclocarya paliurus. The method comprises preparation of a test sample solution, preparation of a control product, establishment of a control fingerprint spectrum and calibration of fingerprint spectrum control peaks. According to structural property characteristics of active components, such as terpenoids, flavonoids, steroids, tannins, organic acids, alkaloids and the like, contained in the cyclocarya paliurus, the best mobile phase composition, elution way, detection wavelength, chromatographic column, column temperature and other analysis conditions are screened through a lot of experiments; many experiments verify and show that the method for detecting the fingerprint spectrum of the cyclocarya paliurus can comprehensively, objectively and accurately detect and evaluate the quality of the cyclocarya paliurus, and has important significance on ensuring clinical efficacy.