4 results about "Leukocyte esterase" patented technology

The invention relates to the technical field of biology, in particular to leukocyte esterase detection test paper and a preparation method thereof. Based on the principle of a substrate and a color developing agent, the color developing depth is in direct proportion to the concentration of the esterase, so that the concentration of the leukocyte esterase in a detection sample can be judged according to the color developing depth; meanwhile, under the auxiliary action of calcium chloride and other materials, the detection distinction degree, the detection accuracy and the detection result stability are further improved. The leukocyte esterase test paper provided by the invention is simple in sample detection method, more stable in product performance, longer in validity period and wide in application range, can be used as an auxiliary evaluation index for vagina inflammation, and is combined with a cleanliness index and the like to jointly evaluate the vagina environment.

The system comprises a laminated 5 diagnostic strip including a backing layer (105), an absorbent pad layer (106) configured for controlled capillary transport of milk, and a reagent deposit layer (107) defining multiple spatially separated dry reagent zones. The reagent zones include a pH indicator zone (101), a leukocyte esterase detection zone (102), a hemoglobin detection zone (103), and a nitrite detection zone (104), each adapted 10 for operation in the milk matrix. A calibrated sample application point (108) receives a predefined volume of untreated milk and fluidically communicates the sample to the reagent zones via the absorbent pad layer. Colorimetric reactions develop in a result window area (109), enabling simultaneous visual interpretation without analytical instruments, typically within three minutes. The invention further 15 includes a moisture-controlled storage container comprising desiccant elements (204, 205), a reference color chart (208), and a calibrated pipette (209), ensuring reagent stability and standardized use.

The invention provides a quality control product for detecting vaginal secretion and a preparation method of the quality control product. The quality control product comprises a negative quality control product and a positive quality control product; the positive quality control product comprises 20-200 mM of a second basic buffer solution, 1-5% of a second enzyme protective agent, 0-0.04% of lactic acid, 0-0.005% of hydrogen peroxide, 0.01-0.1 U / mL of a second leukocyte esterase item, 0.02-0.1 U / mL of sialidase, 0.05-0.5 U / mL of a second N-acetylglucosaminidase item, 50-200 U / mL of a second oxidase item, 2-40 U / mL of a second coagulase item, 50-200 U / mL of beta-glucuronidase, 5-20 U / mL of proline aminopeptidase, 1-5 U / mL of a second alkaline phosphatase item and 0.2-0.2. And 1% of bovine serum albumin.

The application discloses a preparation method of a nystatin fatty acid ester vaginal suppository responding to inflammation, a product and application thereof, and belongs to the field of medicine. The preparation method comprises the following steps: dissolving fatty acid in a solvent, adding a catalyst, uniformly mixing, and activating a carboxyl group of the fatty acid, adding nystatin, and stirring to obtain nystatin fatty acid ester. The fatty acid is saturated or unsaturated fatty acid with a carbon chain length of 4-17 carbons. The application effectively solves the problems that nystatin is unevenly distributed in the suppository matrix and is prone to suppository sedimentation and stratification. Meanwhile, the nystatin fatty acid ester disclosed by the application can be hydrolyzed under the action of leukocyte esterase at an inflammation site of a vagina, release nystatin raw medicine, and play a bactericidal role responding to inflammation. In the non-inflammation area, the nystatin fatty acid ester is hardly hydrolyzed into nystatin, and thus the local adverse reaction of the vagina caused by nystatin can be effectively reduced.