30 results about "Leukocyte esterase" patented technology

A diagnostic test kit for detecting the presence or absence of an enzyme (e.g., leukocyte esterase) within a test sample is provided. The test kit comprises a substrate that is capable of being cleaved in the presence of the enzyme to release a nucleophilic aromatic compound. The kit also comprises a lateral flow device that comprises a chromatographic medium. The chromatographic medium defines a detection zone within which is contained a first reagent (e.g., diazonium ion) that is capable of reacting with the nucleophilic aromatic compound to form a second reagent (e.g., aromatic azo compound). The second reagent exhibits a color that is different than the color of the first reagent. The lateral flow device also includes an absorbent material located adjacent to the chromatographic medium, the absorbent material receiving the test sample after flowing through the chromatographic medium.

The invention relates to a test paper tape which can be used in the gynecological multiprogramming dry-chemistry joint detection. The test paper tape provided by the utility model comprises a dry-chemistry multiprogramming detection test paper tape which consists of a plastic substrate tape and various solidified regent blocks, and sample diluent. The dry regent blocks include combinations formed by increasing or decreasing at least three or more regent blocks of a Ph test regent block, a lactic acid regent block, a hydrogen peroxide concentration regent block, a leukocyte esterase concentration regent block, a neuraminidase activity regent block, an amine test regent block, a proline aminopeptidase substrate reagent block, an oxidase substrate reagent block, a N-acetylamine hexosidase substrate reagent block, a trichomonas specific protease substrate hydrolysis reagent block. the gynecological multiprogramming dry-chemistry joint detection test paper test provided by the utility model can detect Ph, lactic acid, hydrogen peroxide, leukocyte esterase, neuraminidase, amine test, proline aminopeptidase, oxidase, N-acetylamine hexosidase and trichomonas specific protease which are contained in leucorrhea sample at the same time, and can accurately reflect the microorganism environment of a women reproductive tract, the cleanness of leucorrhea secretion and the conditions of Candida albicans, trichomonas, gonococcus and the pathogens of bacterial vaginosis, thereby making the gynecological trichomonas detection more comprehensive; besides, the test paper tape provided by the utility model can be used easily and conveniently, and results can be obtained quickly. If the test paper tape is used together with a gynecological dry-chemistry analyzer, the operation can become easier and the result can be obtained more quickly.

The invention discloses a bacterial vaginosis joint detecting reagent, which comprises a hydrogen peroxide reaction pad foam fluid, a neuraminidase reaction pad foam fluid, a leukocyte esterase reaction pad foam fluid and a pH reaction pad foam fluid. Compared with the prior art, the bacterial vaginosis joint detecting reagent has the advantages of simultaneous detection of multiple data sets, respective reflection of microecological environment, pathogenic bacteria and secretions situations, more comprehensive and reliable detection, simple and quick operation, no need of instrument and equipment, and lower cost.

The invention aims to provide a combined detection kit for vaginitis, capable of evaluating vaginal micro-ecological environment, vaginal cleanliness, and pathogens of bacterial vaginosis, Candida vaginitis, trichomonas vaginitis of the tested person simultaneously. Ihe following technical scheme adopted by the invention, a combined detection kit for vaginitis is provided and comprises a reaction device, diluent and chromogenic liquid, the reaction device is a hole type box-shaped reaction device with six reacting blind holes, in which a hydrogen peroxide concentration detection reagent pad, a leukocyte esterase detection reagent pad, a neuraminidase detection reagent pad, a proline aminopeptidase detection reagent pad, an acetyl glucosaminidase detection reagent pad and pH detection test paper are arranged respectively. Through the combined detection, the vaginitis caused by most of the BV (Bacterial Vaginosis) pathogens, the Candida albicans and the trichomonas can be detected, BV missing detection caused by the simple detection for one kind of enzyme activity is avoided largely, and the detection reliability and accuracy are increased.

The invention discloses a vaginitis test kit which comprises a reaction device, a sampling test tube, a straw, joint test diluent, joint test chromogenic reagent, joint test stop solution, a user manual and a joint test colourimetric card, wherein a hydrogen peroxide reaction hole, a leukocyte esterase reaction hole, a sialic acid glucoside enzyme reaction hole, a beta- glucuronic acid enzyme reaction hole, a P glucosidase reation hole and a pH value hole; relevant reaction bases are arranged in the reaction holes; reach reaction base comprises a corresponding reaction substrate curing layer,a chromogenic promotional layer and a chemical inert carrier layer. The invention also provides a preparation method of the test kit and the preparation method of a novel chemical carrier. The test kit can be used for distinguishing bacterial vaginosis and vaginitis, and can further identify aerobic / anaerobic bacteria, facultative anaerobic bacteria and other flora in vaginal secretion. The method is simple and quick to operate, has high accuracy, and is applicable to clinical practice, particularly hospital outpatient practice.

The invention discloses a combined detection kit for seminal plasma. The nine major indexes of pH value, leukocyte esterase, lecithin body, citric acid, zinc, acid phosphatase, elastase, fructose and neutral alpha-glucosidase in the seminal plasma can be rapidly, accurately, simply, conveniently and practically detected at one time in a combined way, functions of glands such as the prostate, the epididymis and the vesicula seminalis can be comprehensively evaluated, detailed related information is provided for the clinical diagnosis and treatment of caused related diseases such as prostatitis, reproductive system inflammation, sexual dysfunction and sterility infertility, a market gaps are filled, and the combined detection kit is easy to clinically popularize.

The invention aims to provide a joint vaginitis detection kit which is convenient, quick and sensitive to operate, does not need any high-end instrument and equipment, and has low price. The technical scheme of the invention is to provide the joint vaginitis detection kit comprising a clamp-shaped reaction device with four dry chemical reaction blind holes, diluent and chromogenic reagent; and the four blind holes are respectively provided with a hydrogen peroxide concentration determination test reagent pad, a sialic acid glucoside enzyme activity test reagent pad, a leukocyte esterase activity determination test reagent pad and a vaginal fluid pH test reagent pad. The joint vaginitis detection kit can simultaneously test four indexes, i.e. the pH value of vaginal secretion, hydrogen peroxide, sialic acid glucoside enzyme and leukocyte esterase. Through the four indexes, the micro-ecological environment of vagina is determined, and the joint vaginitis detection kit is for identifying bacterial vaginosis of normal vaginal flora and bacteria groups.

The invention discloses a prostatitis joint-detection kit which comprises a sample diluent, a chromogenic reagent and a detection card body provided with six reaction holes, wherein the six reaction holes of the detection card body are internally and respectively provided with solid reagents used for detecting lecithin bodies, leukocyte esterase, citric acid, zinc, acid phosphatase and pH value. The prostatitis joint-detection kit has the advantages of being capable of detecting six representative indexes of the prostatitis together with no need of the microscopic examination. The prostatitis joint-detection kit can judge whether the six indexes are normal or not according to the color changes of the samples in the six reaction holes, efficiently assist the auxiliary diagnosis and the preliminary parting of the prostatitis, and avoid the detection omission caused by the visual fatigue of detection staffs. The prostatitis joint-detection kit is easy to popularize in the clinical application.

The invention discloses a prostatitis joint inspection kit. The kit performs one-time detection to seven representative indexes including pH value, leukocyte esterase, lecithin body, citric acid, zinc, acid phosphatase and elastolytic enzyme used for diagnosing prostatitis, judges whether the indexes are normal according to the changing of colors of samples in seven holes, provides effective help for the correct diagnose of prostatitis, and avoids leak detection caused by visual fatigue of detection personnel. Meanwhile, the kit is simple to operate, and rapid and accurate, does not need special instruments, and is easier for clinical promotion.

The invention discloses a serial gynecological examination kit for bacterial vaginosis, which comprises a kit body; four reaction holes are disposed on the surface of the kit body; the four reaction holes are respectively: a hydrogen peroxide reaction hole, a leukocyte esterase reaction hole, a neuraminidase reaction hole, and a polyamine reaction hole; a hydrogen peroxide active reagent pad, a eukocyte esterase active reagent pad, a neuraminidase active reagent pad, and a polyamine active reagent pad are correspondingly mounted below the four reaction holes; a sealing strip is disposed above the four reaction holes; the kit body is a cuboid. The invention also discloses an application method of the serial gynecological examination kit for bacterial vaginosis, and the kit of the invention has the advantages of small size, high speed, simple operation, stable result, easy interpretation, objective index, and no special equipment requirement.

A diagnostic test kit for detecting the presence or absence of an enzyme (e.g., leukocyte esterase) within a test sample is provided. The test kit comprises a substrate that is capable of being cleaved in the presence of the enzyme to release a nucleophilic aromatic compound. The kit also comprises a lateral flow device that comprises a chromatographic medium. The chromatographic medium defines a detection zone within which is contained a first reagent (e.g., diazonium ion) that is capable of reacting with the nucleophilic aromatic compound to form a second reagent (e.g., aromatic azo compound). The second reagent exhibits a color that is different than the color of the first reagent. The lateral flow device also includes an absorbent material located adjacent to the chromatographic medium, the absorbent material receiving the test sample after flowing through the chromatographic medium.

The invention relates to a luteinizing hormone and bacterial vaginosis joint-test kit, in particular to the luteinizing hormone (LH) and bacterial vaginosis (BV) joint-test kit, and belongs to the technical field of test. The kit comprises an LH immunochromatography test paper tape and one or more of a pH test strip, a sialidase test strip, a leukocyte esterase test strip, a hydrogen peroxide test strip and an amine test strip. Preferably, a combination pad and a chromatography film of the immunochromatography test paper tape are respectively wrapped by a corresponding immune labeled antibody and a test antibody. The LH immunochromatography test paper tape and one or more of the pH test strip, the sialidase test strip, the leukocyte esterase test strip, the hydrogen peroxide test strip and the amine test strip are arranged in parallel or arranged on the upper surface and the lower surface of a bottom plate. According to the luteinizing hormone and bacterial vaginosis joint-test kit, by means of self sampling, LH and bacterial vaginosis can be tested simultaneously, the joint-test result can provide a strong evidence for sound child rearing and reasonable contraception, and the luteinizing hormone and bacterial vaginosis joint-test kit is simple and convenient to achieve and suitable for being popularized and used in a large scale.

The invention discloses an eight-joint detection kit for vaginitis. The eight-joint detection kit is characterized by comprising a reaction plate and (2) a diluent, wherein the reaction plate is provided with reaction holes for detecting the following indexes: beta-glucuronidase, coagulase, hydrogen peroxide, neuraminidase, leukocyte esterase, acetamido-beta-glucosidase, proline aminopeptidase andpH. The kit aims to solve the problem that aerobic bacterial vaginitis, bacterial vaginitis, trichomonas vaginitis and mycotic vaginitis cannot be detected at the same time, and meanwhile, the problem that the kit is poor in detection precision is solved.

The invention discloses a vaginitis test kit which comprises a reaction device, a sampling test tube, a straw, joint test diluent, joint test chromogenic reagent, joint test stop solution, a user manual and a joint test colourimetric card, wherein a hydrogen peroxide reaction hole, a leukocyte esterase reaction hole, a sialic acid glucoside enzyme reaction hole, a beta- glucuronic acid enzyme reaction hole, a P glucosidase reation hole and a pH value hole; relevant reaction bases are arranged in the reaction holes; reach reaction base comprises a corresponding reaction substrate curing layer, a chromogenic promotional layer and a chemical inert carrier layer. The invention also provides a preparation method of the test kit and the preparation method of a novel chemical carrier. The test kit can be used for distinguishing bacterial vaginosis and vaginitis, and can further identify aerobic / anaerobic bacteria, facultative anaerobic bacteria and other flora in vaginal secretion. The method is simple and quick to operate, has high accuracy, and is applicable to clinical practice, particularly hospital outpatient practice.

The invention discloses a quality control material for detecting gynecological secreta, and a preparation method of the quality control material. The quality control material comprises a negative quality control material and a positive quality control material. The quality control material can be used for carrying out quality control on nine items including hydrogen peroxide, lactic acid, oxidase, glucuronidase, N-acetyl hexosaminidase, neuraminidase, leukocyte esterase, proline aminopeptidase and potential of hydrogen, thus solving the problems of the existing quality control material that quality control items are fewer and stability is poor. The quality control material provided by the invention has the advantages of more quality control items, stableness and effectiveness, and can be applied to automatic detection equipment such as a gynecological secreta detector. After being subjected to freeze drying, the negative quality control material and the positive quality control material are dry powder-shaped; the quality control material can be made into a kit form. Furthermore, the preparation method provided by the invention is simple and easy to operate, and production cost is low.

The invention provides a vaginitis multi-detection card which comprises a base plate, at least six sample grooves, a drying agent accommodating groove and a sealing member. The sample grooves are formed in the base plate at intervals, and solidified substances of an optional type of neuraminidase substrate reagents, peroxidase reagents, proline aminopeptidase substrate reagents, N-acetylamino hexosidase substrate reagents, leukocyte esterase substrate reagents, oxidase substrate reagents and PH (potential of hydrogen) substrate reagents are arranged in at least four sample grooves; the drying agent accommodating groove is formed in the base plate, the drying agent accommodating groove and the sample grooves are formed at intervals, and drying agents are arranged the drying agent accommodating groove; the sealing member covers the base plate and is used for sealing the sample grooves and the drying agent accommodating groove. The vaginitis multi-detection card has the advantages that the vaginitis multi-detection card is provided with the clamp groove for accommodating the drying agents, accordingly, the vaginitis multi-detection card still can keep dry after being opened, the performance of the vaginitis multi-detection card can be conveniently guaranteed, and the vaginitis multi-detection card is suitable for large-scale popularization and application.

The present invention relates to the field of infectious diseases. The invention specifically relates to the diagnostic test for acute bacterial pharyngitis. The test is used in screening for Group A Beta Haemolytic Streptococcus by the identifying the presence of leukocyte esterase in the throat. The Leukocyte Esterase Throat Swab Test is compared to the Rapid Step Test for efficiency in terms of fast delivery of results.

The invention discloses a vaginal secretion dry chemical analysis liquid quality control material and a preparation method thereof. A corresponding buffer system is prepared first, and then, with the buffer system as a solvent, a plurality of raw materials of a negative quality control material and a positive quality control material are added and dissolved; when different components are added, the next component can be added after the previous component is completely dissolved; in order to ensure the enzyme activity, a liquid negative quality control material and a liquid positive quality control material are prepared in an environment of 4-15 DEG C, and the effect is better; after the preparation is completed, subpackaging and storage are carried out within 24 hours, and quality control can be carried out on 10 items such as coagulase, hydrogen peroxide, lactic acid, oxidase, glucuronidase, N-acetyl hexosaminidase, sialidase, leukocyte esterase, proline aminopeptidase and pH value at the same time, so that the stability is good, the period is long, the use is convenient and fast, complex step of redissolving before the freeze-dried product is used is omitted, and errors caused by redissolving are reduced; and the liquid quality control material has a good practical application value.

The invention discloses a quality control material for detecting gynecological secreta, and a preparation method of the quality control material. The quality control material comprises a negative quality control material and a positive quality control material. The quality control material can be used for carrying out quality control on nine items including hydrogen peroxide, lactic acid, oxidase, glucuronidase, N-acetyl hexosaminidase, neuraminidase, leukocyte esterase, proline aminopeptidase and potential of hydrogen, thus solving the problems of the existing quality control material that quality control items are fewer and stability is poor. The quality control material provided by the invention has the advantages of more quality control items, stableness and effectiveness, and can be applied to automatic detection equipment such as a gynecological secreta detector. After being subjected to freeze drying, the negative quality control material and the positive quality control material are dry powder-shaped; the quality control material can be made into a kit form. Furthermore, the preparation method provided by the invention is simple and easy to operate, and production cost is low.

The invention discloses a detection reagent card and a kit, which relate to the technical field of disease detection reagents. The detection reagent card disclosed by the invention is provided with a detection tank which can detect sialidase, catalase, leukocyte esterase or pH value index. The present invention also provides a multi-index self-detection kit for bacterial vaginosis comprising the above-mentioned reagent card and self-heating pad. The detection of each index of the reagent card of the present invention is independent of each other, all of which are one-step reaction, no need to add additional color developing solution or stop solution, and the subject only needs to add the collected vaginal secretions to the reagent card for detection; The self-heating pad is introduced into the kit, which does not require additional heating equipment, and the detection conditions are greatly relaxed, which is convenient for subjects to use at home, and is conducive to the promotion of family-based bacterial vaginosis detection.

The invention discloses a prostatitis joint-detection kit which comprises a sample diluent, a chromogenic reagent and a detection card body provided with six reaction holes, wherein the six reaction holes of the detection card body are internally and respectively provided with solid reagents used for detecting lecithin bodies, leukocyte esterase, citric acid, zinc, acid phosphatase and pH value. The prostatitis joint-detection kit has the advantages of being capable of detecting six representative indexes of the prostatitis together with no need of the microscopic examination. The prostatitis joint-detection kit can judge whether the six indexes are normal or not according to the color changes of the samples in the six reaction holes, efficiently assist the auxiliary diagnosis and the preliminary parting of the prostatitis, and avoid the detection omission caused by the visual fatigue of detection staffs. The prostatitis joint-detection kit is easy to popularize in the clinical application.

The present invention relates to the field of infectious diseases. The invention specifically relates to the diagnostic test for acute bacterial pharyngitis. The test is used in screening for Group A Beta Haemolytic Streptococcus by the identifying the presence of leukocyte esterase in the throat. The Leukocyte Esterase Throat Swab Test is compared to the Rapid Step Test for efficiency in terms of fast delivery of results.

Certain embodiments are directed hydrophilic redox substrates for leukocyte esterase (LE). In certain aspects a hydrophilic redox leukocyte esterase substrate or a small molecule enzyme substrate for use in methods and / or devices to electrochemically detect and / or measure LE activity in a sample.

The invention discloses a kit for detecting Mycoplasma genitalium, including two schemes: scheme one is two reagents, including methionine aminopeptidase and leukocyte esterase, wherein the activity of methionine aminopeptidase is detected The enzyme substrate used is a methionine derivative containing chromogen; the enzyme substrate used for detecting leukocyte esterase activity is an ester derivative containing chromogen. The second scheme is three kinds of reagents, that is, on the basis of the first scheme, it also includes a proline aminopeptidase detection reagent, and the enzyme substrate used for detecting the proline aminopeptidase activity is a proline derivative containing a chromogen. The invention detects the enzymes synthesized by the mycoplasma genitalium itself or the enzymes produced by stimulating parasitic cells through the dry chemical enzymatic method to achieve the purpose of detecting the mycoplasma genitalium, and has the advantages of quickness, simplicity, accuracy and the like. Using the kit of the invention to detect mycoplasma genitalium has high sensitivity and specificity, is similar in performance to existing similar detection reagents, and can fully meet the needs of clinical detection.

The invention provides an AV / BV joint detection kit, which is composed of a reaction device, a sample diluent, a chromogenic agent A and a chromogenic agent B, wherein the reaction device is provided with a hydrogen peroxide reaction hole, a sialidase reaction Well, white blood cell esterase reaction well, β-glucuronidase reaction well and coagulation enzyme reaction well, there are corresponding reaction pads in the reaction well. The invention also provides a preparation method of the detection kit. The detection kit of the present invention can directly identify the aerobic bacteria / Anaerobic bacteria and facultative anaerobic bacteria and other flora, the method is simple, fast, and highly accurate, and is suitable for routine clinical use, especially in outpatient clinics.

The invention discloses a prostatitis joint inspection kit. The kit performs one-time detection to seven representative indexes including pH value, leukocyte esterase, lecithin body, citric acid, zinc, acid phosphatase and elastolytic enzyme used for diagnosing prostatitis, judges whether the indexes are normal according to the changing of colors of samples in seven holes, provides effective help for the correct diagnose of prostatitis, and avoids leak detection caused by visual fatigue of detection personnel. Meanwhile, the kit is simple to operate, and rapid and accurate, does not need special instruments, and is easier for clinical promotion.

The invention discloses aerobic bacterial vaginitis test paper and a preparation method thereof. The preparation method comprises the steps of arranging a plurality of carriers on a reaction substrate, after a blank block is reserved on the carriers, respectively infiltrating the rest carriers in a beta-glucuronidase solution, a coagulase solution, a hydrogen peroxide solution, a leukocyte esterase solution and a pH value solution and then performing drying to prepare the aerobic bacterial vaginitis test paper. The aerobic bacterial vaginitis can be comprehensively detected and evaluated according to five items of pH value, leukocyte esterase, hydrogen peroxide, beta-glucuronidase and coagulase; and one-step operation is adopted, a developing solution or a stop solution does not need to be added during detection, operation is simple, misoperation is avoided, and automatic detection is facilitated. The reaction can be detected only by incubating for 5 minutes, the detection time is short, the TAT time is shortened, and the test paper is suitable for clinical test detection, especially hospital outpatient detection and screening.

The invention discloses leukocyte esterase detection test paper, a preparation method and a detection method. The leukocyte esterase detection test paper comprises a substrate, a buffer solution, a stabilizer, a reaction accelerator, a color developing agent, organic acid, an organic solvent, a surfactant and filter paper. The preparation method comprises: respectively preparing two mixed solutions from the obtained substrate and the color developing agent based on preparation requirements, and mixing the two mixed solutions according to a set mixing ratio to obtain a leukocyte esterase solution; and draining filter paper immersed in the leukocyte esterase solution, rotationally and uniformly flowing, drying the filter paper flatly laid on a drying support plate by using an electrothermal blowing drying box, cutting, and hermetically storing to obtain the leukocyte esterase test paper. The obtained leukocyte esterase detection test paper is respectively used for urine leukocyte esterase detection and vaginal secretion leukocyte esterase detection, so that the operation is simple, and the application is wide.

The invention relates to a luteinizing hormone and bacterial vaginosis joint-test kit, in particular to the luteinizing hormone (LH) and bacterial vaginosis (BV) joint-test kit, and belongs to the technical field of test. The kit comprises an LH immunochromatography test paper tape and one or more of a pH test strip, a sialidase test strip, a leukocyte esterase test strip, a hydrogen peroxide test strip and an amine test strip. Preferably, a combination pad and a chromatography film of the immunochromatography test paper tape are respectively wrapped by a corresponding immune labeled antibody and a test antibody. The LH immunochromatography test paper tape and one or more of the pH test strip, the sialidase test strip, the leukocyte esterase test strip, the hydrogen peroxide test strip and the amine test strip are arranged in parallel or arranged on the upper surface and the lower surface of a bottom plate. According to the luteinizing hormone and bacterial vaginosis joint-test kit, by means of self sampling, LH and bacterial vaginosis can be tested simultaneously, the joint-test result can provide a strong evidence for sound child rearing and reasonable contraception, and the luteinizing hormone and bacterial vaginosis joint-test kit is simple and convenient to achieve and suitable for being popularized and used in a large scale.