Aerobic bacterial vaginitis test paper and preparation method thereof

A technology for detecting test strips and vaginitis, which is applied in the direction of biochemical equipment and methods, measuring devices, and microbial determination/inspection, and can solve the problems of incomplete detection and inconvenient operation

Pending Publication Date: 2021-05-18
URIT MEDICAL ELECTRONICS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a test paper for the detection of aerobic vaginitis and its preparation method, aiming to solve the incomplete detection of existing detection me

Method used

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  • Aerobic bacterial vaginitis test paper and preparation method thereof
  • Aerobic bacterial vaginitis test paper and preparation method thereof
  • Aerobic bacterial vaginitis test paper and preparation method thereof

Examples

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preparation example Construction

[0031] In the second aspect, please refer to 4. The present invention also provides a method for preparing an aerobic vaginitis detection test paper, including:

[0032] S101 preparing β-glucuronidase carrier 2;

[0033] S102 preparing coagulase carrier 2;

[0034] S103 preparing leukocyte esterase carrier 2;

[0035] S104 prepares hydrogen peroxide carrier 2;

[0036] S105 prepares pH carrier 2;

[0037] S106 attaching all the carriers 2 to the reaction substrate 1 to complete the preparation.

Embodiment 1

[0040] Dissolve the substrate in a buffer solution with a pH value of 4-9 and a concentration of 0.5mM-10mM, and add 1g / L-10g / L stabilizer and 0.5g / L-5g / L reaction accelerator to form a liquid A, wherein The substrate consists of 0.1g / L~4.0g / L 5-bromo-4-chloro-3-indole-β-D-glucuronide sodium salt, 4-nitrophenyl-β-D-glucopyranoside (PNPG), 5-bromo-4-chloro-3-indolyl-β-D-glucopyranoside, phenolphthalein glucuronide, or more than one. 0.1g / L~4.0g / L color developer, the color developer is a diazonium salt reagent, and the diazonium salt includes 2-methoxy-4-morpholinylbenzenediazonium salt, 2-ethoxy- One of 4-morpholinobenzenediazonium salt and 4-methoxybenzenediazonium salt. 0.2g / L~6.0g / L reaction accelerator. The solvent is one of PBS buffer, citric acid-trisodium citrate buffer, citric acid-TRIS buffer, boric acid-borax buffer and sodium acetate buffer. The reaction accelerator is one or more of magnesium chloride, zinc chloride, calcium chloride, ethylene glycol, sodium alg...

Embodiment 2

[0050] Dissolve the substrate in a buffer solution with a pH value of 4-9 and a concentration of 0.5mM-10mM, and add 1g / L-10g / L stabilizer and 0.5g / L-5g / L reaction accelerator to form A solution. Dissolve the diazonium salt developer in a solution containing 0.5g / L-10g / L organic acid to form liquid B. Mix liquid A and liquid B at a ratio of 1:1 to form a β-glucuronidase solution. Add 5-30ul of β-glucuronidase solution dropwise into reaction well 4 of the punched filter paper, and dry to form β-glucuronidase reaction well 4 .

[0051] Dissolve the substrate in a buffer buffer with a pH value of 3-7 and a concentration of 0.1mM-5mM, add a stabilizer and dissolve to form A solution, and dissolve the diazonium salt developer in a solution containing 0.5g / L-10g / B liquid is formed in L organic acid solution. Add 5-30ul of the coagulase A solution dropwise into the reaction well 4 of the punched filter paper, after drying, add 5-30ul of the coagulase A solution dropwise and dry to...

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Abstract

The invention discloses aerobic bacterial vaginitis test paper and a preparation method thereof. The preparation method comprises the steps of arranging a plurality of carriers on a reaction substrate, after a blank block is reserved on the carriers, respectively infiltrating the rest carriers in a beta-glucuronidase solution, a coagulase solution, a hydrogen peroxide solution, a leukocyte esterase solution and a pH value solution and then performing drying to prepare the aerobic bacterial vaginitis test paper. The aerobic bacterial vaginitis can be comprehensively detected and evaluated according to five items of pH value, leukocyte esterase, hydrogen peroxide, beta-glucuronidase and coagulase; and one-step operation is adopted, a developing solution or a stop solution does not need to be added during detection, operation is simple, misoperation is avoided, and automatic detection is facilitated. The reaction can be detected only by incubating for 5 minutes, the detection time is short, the TAT time is shortened, and the test paper is suitable for clinical test detection, especially hospital outpatient detection and screening.

Description

technical field [0001] The invention relates to the field of in vitro diagnostic reagents, in particular to a test paper for detecting aerobic bacterial vaginitis and a preparation method thereof. Background technique [0002] Donders, a famous Belgian obstetrician and gynecologist, etc. reviewed in 2011 that aerobic vaginitis (Aerobic Vaginitis, AV) is characterized by flora imbalance and vaginal inflammation. Clinically, vaginal Lactobacillus decreases or disappears and is replaced by aerobic bacteria, causing varying degrees of vaginal inflammation. Studies have found that AV is similar to BV, which can easily cause misdiagnosis. Both can cause gynecological and obstetrical diseases, such as pelvic inflammatory disease, premature birth, low birth weight, stillbirth, fetal intrauterine infection, and neonatal mental retardation. [0003] Aerobic vaginitis is a vaginal inflammation in which aerobic bacteria multiply with the absence or reduction of hydrogen peroxide-produc...

Claims

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Application Information

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IPC IPC(8): C12Q1/56C12Q1/44C12Q1/34C12Q1/28
CPCC12Q1/56C12Q1/44C12Q1/34C12Q1/28C12Q2334/52C12Q2326/96G01N2333/96416G01N2333/924G01N2800/36G01N2800/7095
Inventor 蒋庆姣刘云鹏潘军全粟传军
Owner URIT MEDICAL ELECTRONICS CO LTD
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