47results about How to "Detection is easy to automate" patented technology

The invention relates to a fabric flaw detection method. Firstly, a nonlinear gray scale co-occurrence matrix characteristic is constructed in order to utilize characteristic space fully and extract image characteristics effectively, then an optimal scale direction parameter and a self-adaptation flaw segmentation threshold of nonlinear gray scale co-occurrence matrix characteristic extraction are obtained through learning of a flawless fabric image, finally, the obtained parameter is employed to characteristics of an image to be detected, and a flaw area is positioned through characteristic similarity distance measurement. The method can position a fabric flaw area effectively, and noise interference is small.

The invention relates to a co-detection method for salbutamol, estradiol, bisphenol A and stilboestrol in the human urine, which belongs to the technical field of biological engineering. The co-detection method is characterized by including the following steps of collecting the human urine and storing the urine after the urine is centrifugated; adding internal-marked naphthyl hydroxyl heart amine in the urine and then adding methyl alcohol and chlorhydric acid for acidolysis; adding sodium hydroxide solution and sodium acetate buffer solution in the rest urine, then collecting eluent as A liquid and the rest liquid being stand-by through a cleanert PCX solidoid extraction column; taking the rest urine to pass through a cleanert PEP solidoid extraction column and collecting eluent as B liquid; blowing the A liquid to be dry, redissolving the A liquid by acetonitrile and then derivatizating the A liquid; blowing the B liquid to be dry, and extracting twice by sherwood oil after adding potassium carbonate solution; merging the organic phase which is dehydrated and dried through anhydrous sodium sulfate and than repeatedly dissolved by normal hexane; and taking the dry A liquid and the dry B liquid for combined gas chromatography mass spectrometry measurement. The invention has the advantages of simpleness, rapidness, reliable result, high degree of automation and convenient indoctrinated application.

The invention discloses a fluorescence probe and a synthetic method thereof and an application of the fluorescence probe in circulating tumor cell detection. The fluorescence probe comprises a fluorescence group, a cathepsin B substrate and folic acid molecules, the fluorescence group is connected with the cathepsin B substrate through a self-degradation bond, and then is connected with the folic acid molecules; the fluorescence group is excited by the excitation light under a dissociation state, and then is enable covalent connection with other molecules for quenching. The fluorescence probe is the switching-mode fluorescence probe having a spontaneous cascade amplification function, can be used for avoiding complex operation steps of antibody or nucleic acid detection during a circulating tumor cell detection process, is used for an automatic biochemical analyzer as a matching reagent, is in favor of promoting automation detection of tumor cells, and has the advantages of simple and convenient operation, short time consuming, and low apparatus requirement. The fluorescence probe can be used for positioning minimal tumor cells and tubercle in clinical operation, and has wide application prospect and large market value.

The invention discloses a method for detecting the activity of O6-methylguanine-DNA (Deoxyribose Necleic Acid) methyltransferase. The method is that the activity of O6-methylguanine-DNA (Deoxyribose Necleic Acid) methyltransferase is detected by adopting a bioluminescence resonance energy transfer technology and a chemiluminescence technology for the first time, and comprises the steps of incubating streptavidin-luciferase fusion protein and O6-methylguanine-DNA methyltransferase labeled by biotin for 30-60 minutes at the temperature of 0-40 DEG C, then adding O6-methylguanine-DNA methyltransferase antibody labeled by fluorochrome, incubating for 30-60 minutes at the temperature of 0-40 DEG C, then adding a fluorescein substrate, standing for 5-30 minutes at the temperature of 4-30 DEG C, detecting the lighting intensity at the position of 670nm, and further calculating to obtain the activity of O6-methylguanine-DNA methyltransferase. According to the method disclosed by the invention, the operation is simple and convenient, the applicable range is wide, the scattering caused by exogenous exciting light and the interference of backgrounds such as sample matrix fluorescence can be avoided, and the sensitivity and the accuracy are high.

The invention discloses a method for signal amplification and detection on a target deoxyribonucleic acid (DNA) sequence at normal temperature. A DNA signal amplification system is established by using Lambda excision enzyme and a double-tagging fluorescence nucleic acid probe, and the reaction velocity of the Lambda excision enzyme is improved by virtue of the synergistic effect of a fluorescence tagging radial and a mispairing basic group coexisting in a 5' ortho-position. By adopting the method, the target DNA sequence is rapidly, sensitively and specially detected at normal temperature, and convenience is brought for further large-scale and automatic detection. Besides, the method can be independently applied to signal amplification detection, and can be also used with the combination of other template amplification based DNA amplification techniques, such as rolling circle amplification (RCA) and selective polymerase chain reaction (PCR), and various requirements of DNA detection are met.

The invention discloses a method for determining trace terramycin by utilizing a molecularly imprinted immunosensor. When an terramycin molecularly imprinted membrane is formed on the surface of a gold electrode and acts with terramycin molecules, an electrochemical signal of hydroquinone on the gold electrode changes; and when hydrogen peroxide and horseradish peroxidase exist in a detection system, the electrochemical signal has more remarkable change. When terramycin in solution to be measured is utilized to competitively replace original horseradish peroxidase labeled terramycin on the molecularly imprinted membrane, the electrochemical signal can be linearly reduced along with the increase of the concentration of unlabeled terramycin so as to establish an electrochemical analysis method for determining the trace terramycin. A differential pulse voltammetry is used to scan the solution to be measured, the scanning voltage is between -0.6V and 0.8V, and the terramycin is in good linear relation with a peak current value i within the concentration range of between 0 and 8*10-8mol / L. The method overcomes the disadvantages of high complexity and the like existing in the prior art; and the detection limit of the terramycin reaches 10-9mol / L.

The invention discloses an eluting and drying system which comprises a feeding device, an eluting and drying device and a control device. The feeding device is provided with a feeding bucket, a first stirring device, a first conveying pipeline and a first liquid level detecting element. The feeding bucket is provided with a containing cavity for containing chemical materials, a feeding opening, a liquid inlet and a liquid outlet, and the feeding opening, the liquid inlet and the liquid outlet are communicated with the containing cavity. The first conveying pipeline is communicated with the liquid outlet, the first stirring device is connected to the feeding bucket and used for stirring the chemical materials in the containing cavity, and the first liquid level detecting element is connected to the feeding bucket and used for detecting the liquid level of the containing cavity. The eluting and drying device is provided with an eluting and drying machine and a temperature adjusting device. The eluting and drying machine is communicated with the first conveying pipeline, and the temperature adjusting device is connected to the eluting and drying machine and used for adjusting temperature in the eluting and drying machine. The control device is electrically connected to the first stirring device, the temperature adjusting device and the first liquid level detecting element. According to the eluting and drying system, production efficiency can be improved, and standardization and normalization of operation are facilitated.

The invention particularly discloses an indirect Dot-ELISA (enzyme-linked immunosorbent assay) method and application of a blood group antibody. The method comprises the following steps of fixing a nitrocellulose membrane on a 96-hole plate, then directly coating the nitrocellulose membrane with a red cell membrane, and reacting the red cell membrane with a to-be-detected antibody, so as to form an antigen-antibody compound through a second enzyme-labeled antibody. A Dot-ELISA plate can be prepared by utilizing a commercialized ELISA analysis instrument, so that the automatic operation can be achieved, and purposes of saving manpower, reducing personal errors and realizing high-throughput test are achieved. The Dot-enzyme-linked immunosorbent assay method for researching has the advantages that the efficiency is higher than 8 times of that of an indirect Coombs experiment; as the sensitivity is high, the detection accuracies of low-concentration and low-affinity antibodies can be increased, and a blood group detection method and the blood transfusion safety are further improved.

The invention relates to a method for detecting urine metabolites in the technical field of bioengineering, in particular to a method for detecting trimethyl tin chloride and chlorpromazine in the human urine, characterized in that the method comprises the following steps: firstly, centrifuging and storing the collected urine; secondly, taking the urine from the step 1, adding sodium hydroxide, sodium chloride, and ethyl acetate for spiral, centrifugation and extraction; thirdly, aspirating the organic phase, adding anhydrous sodium sulphate for water removal, and concentrating the organic phase by nitrogen at the normal temperature and pressure; and step 4, taking the concentrated liquid from the step 3 for the combined detection of gas chromatography and mass spectrum. The invention is a simple, fast, result-reliable, high-automation, easy-for-industrial application method for detecting trimethyl tin chloride and chlorpromazine in the human urine.

The invention discloses a high-frequency large-wafer bicrystal longitudinal wave probe, which comprises a case, two cable lead wires, two acoustic transmission wedges, two damping blocks and two piezoelectric wafer, wherein the case is divided into two symmetrical cavities by a sound insulation layer at the middle part; the two cable lead wires, the two acoustic transmission wedges, the two damping blocks and the two piezoelectric wafers are symmetrically distributed in the two cavities; the cross section of the acoustic transmission wedge is a right triangle; each acoustic transmission wedgeis fixed on a wear-resistant layer of the bottom surface of the case; each piezoelectric wafer is arranged on the corresponding acoustic transmission wedge in an attached way; each damping block is arranged on the upper surface of the corresponding acoustic transmission wedge; each piezoelectric wafer is connected with cable connectors arranged at the outer part of the case through the cable leadwires; sound absorption materials are fully distributed in the cavities of the case. The probe has the advantages that the frequency is high; the resolution ratio is good; during thin-wall steel platedetection, high detection sensitivity is realized on the lamination defect of a parent metal of a steel plate.

The invention specifically disclosed the indirect DOT-Elisa detection method and application of a blood-type antibody.The method is to fix the nitrate cellulose membrane on the 96-hole plate, and then directly react with the antibody to the antibody to be tested, and the antigen-antibody complex is formed through the enzyme standard two antibodies.The Dot-Elisa board can use commercial ELISA to detect and analyze instruments, which can achieve the purpose of automated operations, to save manpower, reduce human errors, and high-throughput detection.The spots for the immune adsorption measurement method used in this study are more than 8 times the indirect Coombs experiments. High sensitivity can increase the accuracy of the detection of low concentration and the detection of weak relatives and antibodies, and further improve the blood type detection method and improve blood transfusion safety.

The invention relates to the field of valve body machining, in particular to a detection system for flow valve machining and a detection method thereof. The system comprises a conveying system, a feeding system, an appearance detection system, an air tightness detection system, a flow curve detection system, a sorting system, a display system and a control system. The flow valve detection device has the beneficial effects that the flow valve can be conveniently and automatically detected, unqualified products are removed, manpower input can be reduced, and the detection efficiency is improved;Data generated in the detection process is stored through the storage unit and the control system, checking and later tracing are facilitated through the display system, the possibility of false detection and missing detection is further reduced, and defective products are further prevented from flowing into the market.

The invention discloses a real-time fluorescent nucleic acid isothermal amplification detection kit for mycobacterium tuberculosis as well as a special primer and a probe thereof, and belongs to the technical field of biomedical detection. The kit provided by the invention comprises a nucleic acid extracting solution, a detection solution a, a detection solution b, an SAT enzyme solution and the like, the nucleic acid extracting solution comprises an optimally designed specific capture probe extraction probe, target nucleic acid in a pretreated sample can be effectively enriched, extracted and purified, and the detection solution a, the detection solution b and the SAT enzyme solution which are added step by step are combined, and the high-sensitivity detection on the mycobacterium tuberculosis can be realized.

The invention discloses an operating bed inspection device and an inspection method, and relates to the technical field of operating bed inspection. The device comprises a frame, a water tank and a control unit, and a lifting mechanism, an X-direction displacement sensor, a Y-direction displacement sensor and a tension sensor which are connected with the control unit, the water tank is located onthe inner side of the frame and corresponds to the upper portion of the operating bed, and the lifting mechanism is used for driving the water tank to ascend and descend in the vertical direction. Thetension sensor is used for checking the weight of the water tank, the X-direction displacement sensor is used for checking the roll angle of the operating bed, and the Y-direction displacement sensoris used for checking the turnover angle of the operating bed; by implementing the technical scheme, the bearing and each displacement of the operating bed can be automatically inspected, the bearingtest, the displacement test and the fatigue test of the operating bed are carried out, the reliability of operating bed performance inspection is improved, manual recording errors in the detection process can be effectively avoided, accurate online recording is realized, and the operating bed inspection device is simple in structure, convenient to operate, safe and reliable.

The invention belongs to the technical field of automatic electronic detection black box testing, and relates to an automatic black box detection device for an air door opening and closing position state. The main structure comprises a first side supporting plate, a second side supporting plate, a bottom plate, circuit boards, an arc surface, a test space, a photoelectric sensor circuit, an MCU controller circuit, a data bus communication circuit, a main control board, a power line, a connecting line, an outer cover, an outer cover door, a main controller and power supply processing circuits,wherein the width of the circuit board is the same as the length of an arc line corresponding to a 10-degree central angle of the first side supporting plate; the power supply processing circuit, theMCU controller circuit, the photoelectric sensor circuit and the data bus communication circuit on each circuit board form a detection module; and nine detection circuit modules are positioned on thearc surface parallel to the rotating direction of the side wall of the air door, and one detection circuit module is positioned at the bottom of the air door. The measurement range is large, the precision is high, the design improvement efficiency is improved, the principle is scientific, the operation is convenient, safety and reliability are achieved, and the application environment is friendly.

The invention belongs to the field of three-dimensional microwave imaging and provides a microwave imaging-based sandstone aggregate nondestructive testing method. Firstly, sandstone aggregate is placed on a workbench type assembly line; detection equipment with an ''n''-shaped side section and a limited caliber is adopted to detect sandstone aggregate passing through the detection equipment so asto acquire scattering field data; and then the obtained scattering field data is used to realize three-dimensional electromagnetic inverse scattering imaging based on an SOM method to reconstruct a shape and a dielectric constant value of the sandstone aggregate so as to detect whether the sandstone aggregate reaches a standard required by a building or not. In the invention, a unknown scattereris detected by adopting the limited caliber with the ''n''-shaped side section so that actual requirements are met better; the detection process is more convenient and automatic due to a design of anassembly line workbench; and a three-dimensional electromagnetic imaging technology is realized based on an SOM method with two significant advantages of rapid convergence and noise resistance so thatthe method can rapidly and accurately obtain a data result.

The invention discloses a rice management device, and relates to the technical field of rice warehouse management. The problem that rice is affected with damp and cracked is solved. The device specifically comprises a base, first supporting plates, second supporting plates and third supporting plates are arranged on the two sides of the top of the base correspondingly, intermittent rotating mechanisms are arranged on one sides of the first supporting plate, the second supporting plate and the third supporting plate in one set, and the outer walls of the opposite sides of the two third supporting plates are connected through a rotating shaft; the two ends of the rotating shaft are connected with octagonal fixing frames through fixing blocks respectively, clamping grooves are formed in the octagonal outer walls of the octagonal fixing frames, bearing pieces are arranged at the two inner ends of each clamping groove, and two clamping holes are formed in the outer walls of the two inner sides of each clamping groove. According to the device, the temperature and humidity conditions of rice in each storage box are regularly detected through the detection plates, so that corresponding measures can be effectively made aiming at the crack problem of the rice, and the reduction of the rice breakage rate is facilitated.

The invention discloses a safe and efficient rice innovation management platform and a management method thereof, and relates to the technical field of rice warehouse management. The problem that rice is affected with damp and cracked is solved. The device specifically comprises a base, first supporting plates, second supporting plates and third supporting plates are arranged on the two sides of the top of the base correspondingly, intermittent rotating mechanisms are arranged on one sides of the first supporting plate, the second supporting plate and the third supporting plate in one set, and the outer walls of the opposite sides of the two third supporting plates are connected through a rotating shaft; the two ends of the rotating shaft are connected with octagonal fixing frames through fixing blocks respectively, clamping grooves are formed in the octagonal outer walls of the octagonal fixing frames, bearing pieces are arranged at the two inner ends of each clamping groove, and two clamping holes are formed in the outer walls of the two inner sides of each clamping groove. According to the device, the temperature and humidity conditions of rice in each storage box are regularly detected through the detection plates, so that corresponding measures can be effectively made aiming at the crack problem of the rice, and the reduction of the rice breakage rate is facilitated.

The invention discloses rice equipment and a storage method thereof, and relates to the technical field of rice storage management. The problem that rice is affected with damp and cracked is solved. The device specifically comprises a base, first supporting plates, second supporting plates and third supporting plates are arranged on the two sides of the top of the base correspondingly, intermittent rotating mechanisms are arranged on one sides of the first supporting plate, the second supporting plate and the third supporting plate in one set, and the outer walls of the opposite sides of the two third supporting plates are connected through a rotating shaft; the two ends of the rotating shaft are connected with octagonal fixing frames through fixing blocks respectively, clamping grooves are formed in the octagonal outer walls of the octagonal fixing frames, bearing pieces are arranged at the two inner ends of each clamping groove, and two clamping holes are formed in the outer walls of the two inner sides of each clamping groove. According to the device, the temperature and humidity conditions of rice in each storage box are regularly detected through the detection plates, so that corresponding measures can be effectively made aiming at the crack problem of the rice, and the reduction of the rice breakage rate is facilitated.

The invention discloses a real-time fluorescent nucleic acid constant temperature amplification detection kit for Mycobacterium tuberculosis and special primers and probes thereof, belonging to the technical field of biomedical detection. The kit provided by the present invention includes nucleic acid extraction solution, detection solution a, detection solution b, SAT enzyme solution, etc., wherein the nucleic acid extraction solution contains an optimally designed specific capture probe extraction probe, which can be used for pretreated samples. Effective enrichment, extraction and purification of the target nucleic acid in the test solution, combined with the step-by-step addition of detection solution a, detection solution b and SAT enzyme solution, can achieve high-sensitivity detection of Mycobacterium tuberculosis.

The present invention provides a combination of SNP molecular markers for rice genotyping, which consists of 56606 SNP markers. The numbers of the SNP markers are respectively SNP1-SNP56606, and their nucleotide sequences are respectively shown in SEQ ID NO: 1-56606. Show. The combination of SNP molecular markers of the present invention can be used for molecular marker fingerprint analysis of rice variety resources, genotype identification for offspring of hybrid populations, identification of variety authenticity, analysis and screening of genetic background of breeding materials, and correlation of agronomic traits Analysis has broad application prospects.

The invention discloses a rice management device and a management method thereof, and relates to the technical field of rice warehouse management. The problem that rice is affected with damp and cracked is solved. The device specifically comprises a base, first supporting plates, second supporting plates and third supporting plates are arranged on the two sides of the top of the base correspondingly, intermittent rotating mechanisms are arranged on one sides of the first supporting plate, the second supporting plate and the third supporting plate in one set, and the outer walls of the opposite sides of the two third supporting plates are connected through a rotating shaft; the two ends of the rotating shaft are connected with octagonal fixing frames through fixing blocks respectively, clamping grooves are formed in the octagonal outer walls of the octagonal fixing frames, bearing pieces are arranged at the two inner ends of each clamping groove, and two clamping holes are formed in the outer walls of the two inner sides of each clamping groove. According to the device, the temperature and humidity conditions of rice in each storage box are regularly detected through the detection plates, so that corresponding measures can be effectively made aiming at the crack problem of the rice, and the reduction of the rice breakage rate is facilitated.

The invention discloses a method for detecting the activity of O6-methylguanine-DNA (Deoxyribose Necleic Acid) methyltransferase. The method is that the activity of O6-methylguanine-DNA (Deoxyribose Necleic Acid) methyltransferase is detected by adopting a bioluminescence resonance energy transfer technology and a chemiluminescence technology for the first time, and comprises the steps of incubating streptavidin-luciferase fusion protein and O6-methylguanine-DNA methyltransferase labeled by biotin for 30-60 minutes at the temperature of 0-40 DEG C, then adding O6-methylguanine-DNA methyltransferase antibody labeled by fluorochrome, incubating for 30-60 minutes at the temperature of 0-40 DEG C, then adding a fluorescein substrate, standing for 5-30 minutes at the temperature of 4-30 DEG C, detecting the lighting intensity at the position of 670nm, and further calculating to obtain the activity of O6-methylguanine-DNA methyltransferase. According to the method disclosed by the invention, the operation is simple and convenient, the applicable range is wide, the scattering caused by exogenous exciting light and the interference of backgrounds such as sample matrix fluorescence can be avoided, and the sensitivity and the accuracy are high.

The invention belongs to the technical field of electronic detection black box automatic testing, and relates to a black box automatic detection device for the opening and closing position of a damper. The main structure includes a first side support plate, a second side support plate, a bottom plate, a circuit board, an arc surface, and a test space , photoelectric sensor circuit, MCU controller circuit, data bus communication circuit, main control board, power line, connection line, outer cover, outer cover door, main controller and power processing circuit, the width of the circuit board is 10 times that of the first side support plate °The central angle corresponds to the same arc length, the power processing circuit, MCU controller circuit, photoelectric sensor circuit, and data bus communication circuit on each circuit board form a detection module, and the nine detection circuit modules are located parallel to the rotation direction of the damper side wall On the curved surface, one road is located at the bottom of the damper; the measurement range is large, the precision is high, the efficiency of design improvement is improved, the principle is scientific, the operation is convenient, safe and reliable, and the application environment is friendly.

The invention provides an automatic detection method for a GNSS receiver, and the method comprises the following steps: building PC server software for automatic detection of the GNSS receiver; enabling the GNSS receiver to be connected with a PC server; building a TCP communication socket, enabling the TCP communication socket to be in data communication with the software of a PC server side, and carrying out data processing; receiving the detection information of the PC server for the GNSS receiver, and enabling a detection result to be transmitted to the PC server. The method achieves the start of the receiver and the communication with the server software under the cooperation of the PC software, achieves the automatic detection of all modules of the receiver through the coordinative cooperation of the PC software with the receiver firmware, can improve the work efficiency through automatic detection, completes the detection of a plurality of machines quickly and conveniently, and reduces the manpower cost.

The invention relates to a system and method for detecting the butting state of an unmanned surface vehicle water collecting device based on vision, and discloses a system and method applied to an unmanned water quality detection vehicle to perform real-time detection on the butting state of a water collecting device. The detection system comprises a video collection module, a wireless image transmission module and a terminal processing and display module. The detection method comprises the steps that the video collection module collects an image, the wireless image transmission module transmits the image to the terminal processing and display module, and the terminal processing and display module receives the image and then operates a region-of-interest threshold detection algorithm to process and display the image and finally acquires a result on whether the butt-joint is successful or not. The system disclosed by the invention is easy to set up and operate and low in cost, realizesautomatic detection for the butting state of a water collecting device butting mechanism on the unmanned water quality detection vehicle, improves the automation degree of the unmanned water quality detection vehicle, and is more intelligent and efficient.

The invention provides a micro leakage flow automatic detection device which is used for detecting the leakage rate between a first cavity and a second cavity and comprises a liquid source, a fluid pipeline control device and a metering device. The output end of the liquid source is communicated with any one of the first cavity and the second cavity through a fluid pipeline, the other cavity is a testing cavity, the liquid source injects fluid into the cavity connected with the liquid source, the fluid leaks into the testing cavity from the inside, and during testing, the electromagnetic valve is closed so that the internal leakage fluid can only flow into the metering device. After the test is finished, the electromagnetic valve is opened to discharge the leakage fluid, and the metering device is emptied to facilitate the next detection; according to the invention, the automatic detection of the micro leakage flow can be realized without manual intervention, the detection efficiency and precision are greatly improved, and the testable object and working condition range is wider.

The invention discloses a traction device convenient to lock for a motor test, and relates to the technical field of motor test traction. The problem that the motor test operation process is tedious is solved. The device specifically comprises a bottom plate; the outer wall of the top end of the bottom plate is fixedly connected with a detection table through screws; the outer wall of one side ofthe detection table is fixedly connected with a conveying belt through screws; uniformly distributed identification areas are arranged on the outer wall of one side of the conveying belt; locking plates are placed on the outer walls of the tops of the identification areas; the top outer walls of the locking plates are fixedly connected with test motors; L-shaped connecting rods are fixedly connected to the outer walls of sides of output shafts of the test motors; the top outer wall of the bottom plate is fixedly connected with supporting rods through screws. By arranging the detection table, the conveying belt, the locking plates, the test motors, the L-shaped connecting rods, the rotating motors, rotating discs and traction rods, the device automatically detects the motor, is convenient and fast, and can perform test detection on a large number of motors, and the practicability of the device is improved.

The invention discloses a method for detecting long non-coding RNA in urine of prostate cancer patients by using digital PCR. The method comprises the steps that the urine of an object to be detectedis used as a detection sample, and a digital PCR method is adopted to detect specific lncRNA of prostate cancer in urine. The method uses digital PCR to detect lncRNA in urine samples. The content oflncRNA in the urine samples is relatively low, and therefore compared with traditional fluorescence quantitative PCR, the method has the advantages of being more sensitive in detection, lower in detection limit and capable of performing absolute quantification. The method uses a urine sample of a patient for detection. Compared with a traditional blood sample or tumor tissue sample, the detectionsample is easier to obtain and less harmful to the patient. The method is simple in operation, procedures are simplified, repetition is easy, automatic detection is easy, the detection is sensitive, and the detection limit is low.