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Method for detecting trimethyl tin chloride and thorazine in human urine

A technology of trimethyl tin chloride and detection of human body, applied in the field of bioengineering, can solve the problems of neglecting the comprehensiveness and comprehensiveness of detection types, unfavorable industrialization to promote the general population, and the degree of contact with difficult pollutants, and achieves convenience and ease. The effect of obtaining, the method is convenient, and the detection cost is low

Inactive Publication Date: 2009-07-22
陈枢青 +2
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Problems solved by technology

By consulting the literature, we found that there are many methods used by domestic scholars to study the internal exposure metabolites of environmental pollutants trimethyltin chloride and chlorpromazine, including GC, HPLC, GC-MS, enzyme-linked immunosorbent assay etc.; the types of instruments used in the method are also different. Taking GC-MS as an example, a combination of Shimadzu GC-14A and American Agilent 6890N-5973MSD are used; there is no special organization for environmental pollutants and its metabolites for detection of human exposure
Most of the research is dedicated to the detection of single pollutants, and the methods used for the same substance are different, and the focus is on improving the recovery rate of pollutants, ignoring the comprehensiveness and comprehensiveness of the detection types, and the detection cost is high. It is difficult to systematically and comprehensively evaluate the degree of pollutant exposure of the human body, which is not conducive to the promotion of industrialization to the general population

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  • Method for detecting trimethyl tin chloride and thorazine in human urine
  • Method for detecting trimethyl tin chloride and thorazine in human urine
  • Method for detecting trimethyl tin chloride and thorazine in human urine

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Embodiment 1

[0032] Take 3 urine samples stored at -20°C, marked as No. 1, 2, and 3 respectively, centrifuge after natural thawing, and perform the following experimental procedures at the same time: draw 10ml of urine samples into the extraction bottle, add 5% NaOH200ul, 3g chlorine Add sodium chloride, then add 10ml ethyl acetate for extraction, vortex at 1500rpm for 5min, centrifuge at 3000rpm for 4min, suck out the organic phase, add 5g of anhydrous sodium sulfate to remove water, concentrate with nitrogen under normal temperature and pressure, and finally settle to 500ul after concentration Injection analysis. Take 1ul of the reaction solution for gas chromatography-mass spectrometry determination. The GC / MS conditions are: DB-5ms column, EI ion source, voltage of 70eV, ion source temperature of 230°C, and injection port temperature of 280°C. The experimental results were collected by SIM. The results are attached figure 1 , 2 , 3 shown. figure 1 It is the SIM chromatogram of uri...

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Abstract

The invention relates to a method for detecting urine metabolites in the technical field of bioengineering, in particular to a method for detecting trimethyl tin chloride and chlorpromazine in the human urine, characterized in that the method comprises the following steps: firstly, centrifuging and storing the collected urine; secondly, taking the urine from the step 1, adding sodium hydroxide, sodium chloride, and ethyl acetate for spiral, centrifugation and extraction; thirdly, aspirating the organic phase, adding anhydrous sodium sulphate for water removal, and concentrating the organic phase by nitrogen at the normal temperature and pressure; and step 4, taking the concentrated liquid from the step 3 for the combined detection of gas chromatography and mass spectrum. The invention is a simple, fast, result-reliable, high-automation, easy-for-industrial application method for detecting trimethyl tin chloride and chlorpromazine in the human urine.

Description

technical field [0001] The invention relates to a method for detecting urine metabolites in the technical field of bioengineering, in particular to a method for detecting trimethyltin chloride and chlorpromazine in human urine. Background technique [0002] Trimethyltin chloride is a crystalline solid with a rotten grass smell and is volatile at room temperature. The trimethyltin chloride encountered in industry is mainly derived from organotin plastic stabilizers. Trimethyltin chloride is a by-product produced in the production of organotin stabilizers. It is highly toxic and can pass through the respiratory tract, digestive tract and The skin and mucous membranes enter the body. Organotin plastics used in daily life will contain trimethyltin chloride, the probability of human exposure is very high, and there is considerable harm. [0003] Chlorpromazine is a milky white powder with slight odor and bitter taste. It is hygroscopic and changes color when exposed to light. T...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/78G01N1/28
Inventor 吴鸳鸯陈枢青
Owner 陈枢青
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