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Co-detection method for salbutamol, estradiol, bisphenol A and diethyl stilbestrol in human urine

A technology of albuterol and diethylstilbestrol, which is applied in the field of bioengineering to achieve the effects of convenient method, low detection cost and high detection sensitivity

Active Publication Date: 2009-08-05
HANGZHOU ENSHI GENE TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There are many studies on the detection of pollutants in academia, but most of them focus on the measurement of exposure outside the environment

Method used

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  • Co-detection method for salbutamol, estradiol, bisphenol A and diethyl stilbestrol in human urine
  • Co-detection method for salbutamol, estradiol, bisphenol A and diethyl stilbestrol in human urine

Examples

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Embodiment 1

[0042] Take 2 urine samples stored at -20°C, mark them as No. 1 and No. 2, centrifuge after natural thawing, and carry out the following experimental procedures at the same time: take 5ml of the supernatant urine sample, add 200ul 10μg / ml internal standard naphthalene hydroxyl Amine, 1ml methanol, 0.3ml 6M concentrated hydrochloric acid, put at 80°C for 10min, then add 180ul 5M sodium hydroxide, 600ul sodium acetate buffer solution (PH5.0), and pass through the column.

[0043]First pass through column 1 cleanert PCX: use 2ml of methanol and 2ml of 0.1M HCl to activate the pretreatment; pass the sample at a flow rate of 8ml / min, and at the same time connect the waste liquid to pass through column 2; wash the column with 2ml of 0.1M HCl and 2ml of methanol; Elute with 2ml of chloroform / isopropanol / 32% ammonia water [80:2:2 v:v:v] at a flow rate of 6ml / min to obtain eluent A; after drying, add 50ul of acetonitrile to redissolve, then add 50ul of MSTFA , 60° C., derivatized for 3...

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Abstract

The invention relates to a co-detection method for salbutamol, estradiol, bisphenol A and stilboestrol in the human urine, which belongs to the technical field of biological engineering. The co-detection method is characterized by including the following steps of collecting the human urine and storing the urine after the urine is centrifugated; adding internal-marked naphthyl hydroxyl heart amine in the urine and then adding methyl alcohol and chlorhydric acid for acidolysis; adding sodium hydroxide solution and sodium acetate buffer solution in the rest urine, then collecting eluent as A liquid and the rest liquid being stand-by through a cleanert PCX solidoid extraction column; taking the rest urine to pass through a cleanert PEP solidoid extraction column and collecting eluent as B liquid; blowing the A liquid to be dry, redissolving the A liquid by acetonitrile and then derivatizating the A liquid; blowing the B liquid to be dry, and extracting twice by sherwood oil after adding potassium carbonate solution; merging the organic phase which is dehydrated and dried through anhydrous sodium sulfate and than repeatedly dissolved by normal hexane; and taking the dry A liquid and the dry B liquid for combined gas chromatography mass spectrometry measurement. The invention has the advantages of simpleness, rapidness, reliable result, high degree of automation and convenient indoctrinated application.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a co-detection method for salbutamol, estradiol, bisphenol A and diethylstilbestrol in human urine. Background technique [0002] Estradiol is a steroidal anabolic hormone, which has different effects on the metabolism of sugar, fat, protein, salt and other substances in the body. Estradiol is often used in animal husbandry to improve animal weight and feeding efficiency. Estradiol is an endogenous (natural) hormone, while BPA and diethylstilbestrol are estrogen analogs. Environmental estrogen belongs to environmental endocrine disruptors. Its molecular structure is similar to estrogen, and it cannot be distinguished after entering the body, so it has estrogenic effects in the body. After exogenous estrogen and its analogs enter animals or humans, they can interfere with the synthesis, release, operation, metabolism, and combination of normal endocrine substances in the b...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N1/28
Inventor 吴鸳鸯陈枢青
Owner HANGZHOU ENSHI GENE TECH DEV
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