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Method for determining trace terramycin by utilizing molecularly imprinted immunosensor

An immunosensor and molecular imprinting technology, which is applied in biological testing, material electrochemical variables, material inspection products, etc., can solve problems that have not been reported and achieve high sensitivity

Active Publication Date: 2010-05-19
南京浦口科创投资集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The reported imprinted sensors are used for the detection of more than a dozen pesticides such as atrazine, diquat, glyphosate, parathion, chloramphenicol, etc. There is no report on the detection of trace oxytetracycline

Method used

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  • Method for determining trace terramycin by utilizing molecularly imprinted immunosensor
  • Method for determining trace terramycin by utilizing molecularly imprinted immunosensor
  • Method for determining trace terramycin by utilizing molecularly imprinted immunosensor

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Experimental program
Comparison scheme
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Embodiment

[0022] 1. Treatment of gold electrodes:

[0023] The gold electrode was polished with 1.0 μm, 0.3 μm and 0.05 μm alumina powder in turn, washed in nitric acid, absolute ethanol, and pure water with a volume ratio of 1:1, and ultrasonically washed for 5 minutes after taking it out.

[0024] 2. Preparation of oxytetracycline molecularly imprinted immunosensor

[0025] Measure 0.5mL 7×10 -4 mol / L catechol, 2.5mL 3×10 -4 mol / L oxytetracycline solution and 7mL acetic acid-sodium acetate buffer solution with a pH=5.2 concentration of 0.1mol / L, stir well and then use cyclic voltammetry at a speed of 50mV / s between 0 and 0.8V By scanning 30 circles continuously, the oxytetracycline molecularly imprinted immunosensor can be prepared.

[0026] 3. Detection method

[0027] Take a 15mL small beaker, add 10mL 0.1mol / L containing 5×10 -4 mol / L hydroquinone in phosphate buffered saline solution. Immerse the oxytetracycline molecularly imprinted immunosensor fully absorbed in the horser...

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Abstract

The invention discloses a method for determining trace terramycin by utilizing a molecularly imprinted immunosensor. When an terramycin molecularly imprinted membrane is formed on the surface of a gold electrode and acts with terramycin molecules, an electrochemical signal of hydroquinone on the gold electrode changes; and when hydrogen peroxide and horseradish peroxidase exist in a detection system, the electrochemical signal has more remarkable change. When terramycin in solution to be measured is utilized to competitively replace original horseradish peroxidase labeled terramycin on the molecularly imprinted membrane, the electrochemical signal can be linearly reduced along with the increase of the concentration of unlabeled terramycin so as to establish an electrochemical analysis method for determining the trace terramycin. A differential pulse voltammetry is used to scan the solution to be measured, the scanning voltage is between -0.6V and 0.8V, and the terramycin is in good linear relation with a peak current value i within the concentration range of between 0 and 8*10-8mol / L. The method overcomes the disadvantages of high complexity and the like existing in the prior art; and the detection limit of the terramycin reaches 10-9mol / L.

Description

technical field [0001] The invention relates to a method for rapidly measuring trace oxytetracycline by using an electrochemical immunosensor combined with molecular imprinting technology and immunological technology. Background technique [0002] Oxytetracycline is a tetracycline antibiotic drug. It is one of the antibiotics with the longest production and clinical use. Its chemical structure is a hydrogenated tetracene ring derivative. It is well absorbed orally, widely distributed in the body, and can be enriched in liver tissue. , causing liver damage. Oxytetracycline is often added to animal feed to promote animal growth and prevent various diseases. If the drug withdrawal period is not strictly controlled, it is easy to cause residues in animal meat and tissues, which will cause harm to human health. Therefore, the world All countries have strict limit requirements for tetracycline residues in livestock and poultry meat, so it is very meaningful to study a detection m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53G01N27/27G01N27/48
Inventor 李建平蒋复阳魏小平
Owner 南京浦口科创投资集团有限公司
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