A kit for detecting bacterial flora in vaginal secretions and its preparation method

A technology of vaginal secretions and kits, which is applied in the field of kits for detecting microorganisms and its preparation, and can solve problems such as uncomfortable clinical routines and complex AV scores

Active Publication Date: 2011-12-21
北京中生金域诊断技术股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the AV score is a necessary index, but the AV score is very complicated. It needs to comprehensively integrate the indicators such as the grade of lactobacilli in vaginal secretions, the number of white blood cells, the proportion of toxic white blood cells, and the number of background colonies. It is not suitable for clinical routine, especially in outpatient clinics. etc. use

Method used

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  • A kit for detecting bacterial flora in vaginal secretions and its preparation method
  • A kit for detecting bacterial flora in vaginal secretions and its preparation method
  • A kit for detecting bacterial flora in vaginal secretions and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0098] Example 1 Preparation of AV / BV combined assay kit

[0099] 1. Preparation of hydrogen peroxide reaction pad:

[0100] Solution A: Diethylamino-p-phenylenediamine 10mg

[0101] α-Naphthol 20mg

[0102] Absolute ethanol 10ml

[0103] Solution B: horseradish peroxidase 10000 units

[0104] Inactivated purified bovine serum albumin 1g

[0105] Tris-HCl 200mmol / L pH7.0 10ml

[0106] Add 1 μl of solution A dropwise on a Φ4mm chromatography paper pad, and dry it with flowing air at 25°C for 1 hour in the dark, then add 1 μl of solution B on the paper pad dropwise, dry it with flowing air at 25°C for 4 hours in the dark, and seal it. Store at 4°C;

[0107] 2. Preparation of sialidase reaction pad:

[0108] Substrate solution: 0.05g of 5-bromo-4-chloro-3-indole-N-acetylneuraminic acid sodium salt

[0109] Acetate solution 200mmol / L pH5.5 10ml, containing NaCl0.2mol / L

[0110] Add 1 μl of the substrate solution dropwise on a Φ4mm chromatography paper pad, dry it with flo...

Embodiment 2

[0143] Example 2 Using the AV / BV combined assay kit to detect the accuracy of aerobic bacteria / anaerobic bacteria and facultative anaerobic bacteria in vaginal secretions

[0144] 1, respectively adopt clinical and microbiological examination method and AV / BV joint assay kit of the present invention to detect the result of bacterial flora in vaginal secretions as shown in table 2, and accuracy is calculated as follows:

[0145] Sensitivity=D÷(C+D)×100%; Specificity=A÷(A+B)×100%

[0146] Table 2

[0147]

[0148] Five groups of people were tested, including 67 cases of normal reproductive age women (group 1), 51 cases of BV patients (group 2), 44 cases of AV patients (group 3), and 52 cases of VVC patients with Candida albicans vaginitis. Example (the 4th group) and 31 cases (the 5th group) of trichomonal vaginitis TV patient, get vaginal secretions, detect with test kit of the present invention, the result is as shown in table 3:

[0149] table 3

[0150]

[0151] 2. Th...

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Abstract

The invention provides an AV / BV joint detection kit, which is composed of a reaction device, a sample diluent, a chromogenic agent A and a chromogenic agent B, wherein the reaction device is provided with a hydrogen peroxide reaction hole, a sialidase reaction Well, white blood cell esterase reaction well, β-glucuronidase reaction well and coagulation enzyme reaction well, there are corresponding reaction pads in the reaction well. The invention also provides a preparation method of the detection kit. The detection kit of the present invention can directly identify the aerobic bacteria / Anaerobic bacteria and facultative anaerobic bacteria and other flora, the method is simple, fast, and highly accurate, and is suitable for routine clinical use, especially in outpatient clinics.

Description

technical field [0001] The invention relates to a kit for detecting microorganisms and a preparation method thereof, in particular to a kit for detecting aerobic bacteria / anaerobic bacteria and facultative anaerobic bacteria in vaginal secretions and a preparation method thereof. Background technique [0002] Recent studies have found that female bacterial vaginal infections can be divided into two types, one is caused by anaerobic bacteria and facultative anaerobic bacteria, clinically called bacterial vaginosis BV, and the other is caused by aerobic bacteria , Clinically called aerobic vaginitis AV. The pathogens of the two types of vaginitis are different, and the clinical symptoms and treatment options are also different. It is of great significance to distinguish these two types of vaginal infections in the detection of bacterial vaginal infections. [0003] Clinically, vaginal infection is mainly identified based on microbiological tests and clinical manifestations, a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/56C12Q1/44C12Q1/34C12Q1/28C12R1/445C12R1/19C12R1/46C12R1/01
Inventor 王加义郭智芳宋金才张丽梅
Owner 北京中生金域诊断技术股份有限公司
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