Vaginitis test kit and preparation method thereof

A vaginitis and kit technology, applied in biochemical equipment and methods, microbial determination/inspection, material analysis by observing the effect on chemical indicators, etc., can solve the problem of stimulating prostaglandins and tumor cells easily losing contact inhibition. , problems such as reduced adhesion

Active Publication Date: 2012-01-18
JIANGSU BIOPERFECTUS TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, cyclooxygenase and phosphate esters induce uterine cells to produce arachidonic acid, which stimulates the production of prostaglandins involved in labor, leading to premature birth
Sialidase can also hinder the synthesis of bone collagen in fibroblasts, leading to the occurrence of low birth weight infants
[0011] However, it should also be noted that sialic acid is the main component of cell membrane glycoproteins and sugar esters. The free form in normal cells is relatively low, but the activity of sialidase in tumor cells is enhanced, which increases the negative charge on the cell surface, and tumor cells are prone to lose contact inhibition. Unlimited proliferation, but also reduce adhesion, easy to invade and transfer

Method used

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  • Vaginitis test kit and preparation method thereof
  • Vaginitis test kit and preparation method thereof
  • Vaginitis test kit and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0095] Example 1 Preparation of vaginitis detection kit

[0096] 1) Preparation of reaction cards

[0097] The reaction card is made of ABS engineering plastic in a single piece, and the reaction wells are arranged on the right side of the front: hydrogen peroxide reaction wells, leukocyte esterase reaction wells, sialidase reaction wells, β-glucuronidase reaction wells, acetyl Glucosaminidase reaction wells and pH value wells, the reaction wells have a diameter of 4 mm and are circular, and in each reaction well, the corresponding substrate solidification layer, color development promotion layer, and chemically inert carrier are coated according to the following method layers, etc.;

[0098] 2) Preparation of hydrogen peroxide reactive base

[0099] Chemically inert carrier: Mesoporous molecular sieve 10g, magnesium trisilicate 5g, 95% (w / w) ethanol 30ml fully stirred suspension, take 20μl evenly coated in the reaction well, 40 ℃ flowing air drying for 1 hour, medium Pore ...

Embodiment 2

[0138] Embodiment 2 Preparation of vaginitis detection kit of the present invention

[0139] 1) Preparation of reaction cards

[0140] The reaction card is made of ABS engineering plastic in a single piece, and the reaction wells are arranged on the right side of the front: hydrogen peroxide reaction wells, leukocyte esterase reaction wells, sialidase reaction wells, β-glucuronidase reaction wells, acetyl Glucosaminidase reaction wells and pH value wells, the reaction wells have a diameter of 4 mm and are circular, and in each reaction well, the corresponding substrate solidification layer, color development promotion layer, and chemically inert carrier are coated according to the following method layers, etc.;

[0141] 2) Preparation of hydrogen peroxide reactive base

[0142] Chemically inert carrier: Mesoporous molecular sieve 10g, anhydrous calcium sulfate 5g, 95% (w / w) ethanol 30ml fully stirred suspension, take 20μl evenly coated in the reaction well, 40 ℃ flowing air ...

Embodiment 3

[0184] Embodiment 3 The usage method of vaginitis detection kit of the present invention

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PUM

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Abstract

The invention discloses a vaginitis test kit which comprises a reaction device, a sampling test tube, a straw, joint test diluent, joint test chromogenic reagent, joint test stop solution, a user manual and a joint test colourimetric card, wherein a hydrogen peroxide reaction hole, a leukocyte esterase reaction hole, a sialic acid glucoside enzyme reaction hole, a beta- glucuronic acid enzyme reaction hole, a P glucosidase reation hole and a pH value hole; relevant reaction bases are arranged in the reaction holes; reach reaction base comprises a corresponding reaction substrate curing layer,a chromogenic promotional layer and a chemical inert carrier layer. The invention also provides a preparation method of the test kit and the preparation method of a novel chemical carrier. The test kit can be used for distinguishing bacterial vaginosis and vaginitis, and can further identify aerobic/anaerobic bacteria, facultative anaerobic bacteria and other flora in vaginal secretion. The method is simple and quick to operate, has high accuracy, and is applicable to clinical practice, particularly hospital outpatient practice.

Description

technical field [0001] The invention relates to a vaginitis detection kit and a preparation method thereof, in particular to bacterial vaginitis (anaerobic bacteria and facultative anaerobic bacteria vaginitis), aerobic bacteria vaginitis, fungal vaginitis, trichomonas vaginitis Inflammation detection kit and preparation method thereof. Background technique [0002] Microecology is a new subject. With the rapid development of science and technology in recent years, medical microecology has become an independent marginal subject, and has been paid more and more attention by people. From a physiological point of view, the female vagina is a closed body cavity, which contains various components and many bacteria. [0003] The vaginal microecology is mainly composed of three parts. The first is vaginal epithelial cells. The female vaginal wall is composed of stratified squamous non-keratinized epithelial cells, which can continuously proliferate and thicken with the increase of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/28C12Q1/44C12Q1/34C12Q1/04G01N21/80
Inventor 张旭王国强刘中华戚雨张辉
Owner JIANGSU BIOPERFECTUS TECH CO LTD
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