Eight-joint detection kit for vaginitis

A kit and vaginitis technology, applied in the field of in vitro diagnostic reagents, can solve the problem of inability to simultaneously detect aerobic bacterial vaginitis, bacterial vaginosis, trichomonal vaginitis and fungal vaginitis, and the lack of detection accuracy of the kit, etc. problems, to achieve the effect of solving solubility problems, small proportion, and increasing firmness

Pending Publication Date: 2020-09-22
青岛华晶生物技术有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a vaginitis eight joint detection kit, aiming to solve the problem that aerobic vaginitis, bacterial vaginitis, trichomonal vaginitis and fungal vaginitis cannot be detected at the same time. The problem of lack of box detection accuracy

Method used

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  • Eight-joint detection kit for vaginitis
  • Eight-joint detection kit for vaginitis
  • Eight-joint detection kit for vaginitis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Such as Figure 1-Figure 2 A vaginitis eight joint detection kit is shown, including a reaction plate 1, the reaction plate has a reaction well 2 for detecting the following indicators: β-glucuronidase, coagulase, hydrogen peroxide, sialidase, leukocyte ester Enzyme, acetylamino-β-glucosidase, proline aminopeptidase, pH; (2) diluent;

[0044] The substrate reaction reagent of the β-glucuronidase reaction well 2 is an absolute ethanol solution of 0.1-5g / L 5-bromo-4-chloro-3-indole glucuronide, and the β-glucuronidase The stabilizer protection solution of the reaction well is an aqueous solution of 20-50 g / L trehalose.

[0045] The substrate reaction reagent of the coagulase reaction well is glycyl-arginyl-4-methoxy-β-naphthylamide hydrochloride 0.2-4g / L, and the solvent is PBS buffer; the stability of the coagulase reaction well is The agent protection solution is an aqueous solution of 20-50g / L trehalose.

[0046] The substrate reaction reagents of the hydrogen perox...

Embodiment 2

[0058] The solvent of the β-glucuronidase reaction well is ethylene glycol, and the other is the same as in Example 1. The test activity is 1U / mL of β-glucuronidase quality control substance. The comparison results with Example 1 are shown in Table 1.

Embodiment 3

[0060] No HRP enzyme stabilizer is added to the reaction reagent of the hydrogen peroxide reaction well, and the others are the same as in Example 1. The comparison results are shown in Table 2. Table 2 shows the activity of the hydrogen peroxide reaction well under different storage times.

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Abstract

The invention discloses an eight-joint detection kit for vaginitis. The eight-joint detection kit is characterized by comprising a reaction plate and (2) a diluent, wherein the reaction plate is provided with reaction holes for detecting the following indexes: beta-glucuronidase, coagulase, hydrogen peroxide, neuraminidase, leukocyte esterase, acetamido-beta-glucosidase, proline aminopeptidase andpH. The kit aims to solve the problem that aerobic bacterial vaginitis, bacterial vaginitis, trichomonas vaginitis and mycotic vaginitis cannot be detected at the same time, and meanwhile, the problem that the kit is poor in detection precision is solved.

Description

technical field [0001] The invention belongs to the technical field of in vitro diagnostic reagents, and in particular relates to a vaginitis eight joint detection kit. Background technique [0002] The concept of vaginal microecology has been promoted and applied in China for more than 10 years, and the evaluation system has been perfected day by day. The detection content includes two aspects of morphology and function. Morphological detection indicators include vaginal flora density, diversity, dominant bacteria, pathogenic microorganisms (fungus and trichomonas), Nugent score, white blood cell count, etc. Functional detection indicators include pH value, body inflammatory response markers (leukocyte esterase) and biochemical indicators, including detection of metabolites and enzyme activity. [0003] Vaginitis is a common disease in gynecological clinics. Common vaginitis has four types: bacterial vaginitis (BV), fungal vaginitis (VVC), trichomonal vaginitis (TV) and a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/34C12Q1/37C12Q1/28G01N33/50
CPCC12Q1/34C12Q1/37C12Q1/28G01N33/50
Inventor 王训琨路雅丽娄善梅
Owner 青岛华晶生物技术有限公司
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