Leukocyte esterase detection test paper, preparation method and detection method

Abstract

The invention discloses leukocyte esterase detection test paper, a preparation method and a detection method. The leukocyte esterase detection test paper comprises a substrate, a buffer solution, a stabilizer, a reaction accelerator, a color developing agent, organic acid, an organic solvent, a surfactant and filter paper. The preparation method comprises: respectively preparing two mixed solutions from the obtained substrate and the color developing agent based on preparation requirements, and mixing the two mixed solutions according to a set mixing ratio to obtain a leukocyte esterase solution; and draining filter paper immersed in the leukocyte esterase solution, rotationally and uniformly flowing, drying the filter paper flatly laid on a drying support plate by using an electrothermal blowing drying box, cutting, and hermetically storing to obtain the leukocyte esterase test paper. The obtained leukocyte esterase detection test paper is respectively used for urine leukocyte esterase detection and vaginal secretion leukocyte esterase detection, so that the operation is simple, and the application is wide.

Description

technical field [0001] The invention relates to the technical field of in vitro diagnostic reagents, in particular to a leukocyte esterase detection test paper, a preparation method and a detection method. Background technique [0002] Leukocyte esterase is a specific esterase contained in neutrophil cytoplasm. Leukocyte esterase in urine and vaginal secretions is mostly esterase released by neutrophils, and the content is low in a healthy state. Therefore, most of the causes of elevated human leukocyte esterase indicators are inflammation of the genitourinary system. Therefore, the leukocyte esterase item is an important indicator for the detection of urine and vaginal secretions. In urine, urine leukocyte esterase testing is primarily used to diagnose urinary tract infections. Leukocyte esterase detection in the vagina can reflect the degree of vaginal inflammation, and can also be used to evaluate the cleanliness and vaginal mucosal damage in vaginal secretions. Howeve...

AI Technical Summary

Problems solved by technology

However, in the current detection of leukocyte esterase, different detection items must use different detection methods, the operation is complicated, the speed is slow, and the application range is small.

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