The invention discloses a kit for detecting mycoplasma genitalium. The invention provides two schemes; in a scheme I, the kit comprises two reagents, namely methionine aminopeptidase and leucocyte esterase, wherein an enzyme substrate for detecting the activity of the methionine aminopeptidase is a methionine derivative containing a chromogen, and an enzyme substrate for detecting the activity ofthe leucocyte esterase is an ester derivative containing the chromogen; in a scheme II, the kit comprises three reagents, i.e., the kit is also comprises a proline aminopeptidase reagent on the basisof the scheme I, and an enzyme substrate for detecting the activity of proline aminopeptidase is a proline derivative containing the chromogen. According to the kit disclosed by the invention, autosynthetic enzyme or enzyme generated by stimulating parasitic cells during a growth process of the Mg can be detected through a dry chemical enzyme method, the purpose of detecting the mycoplasma genitalium can be achieved, and the kit has the advantages of quickness, simpleness, accuracy and the like. By adopting the kit for detecting the mycoplasma genitalium, the sensitivity and the specificity are higher, properties of the kit are similar to properties of existing like detection reagents, and clinical test needs can be completely met.